Reviewed, UniProtKB/Swiss-Prot Q8TLG9 (CHEB1_METAC)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	MA_3067

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158051
Regions
	Domain	1 – 119	119	Response regulatory
	Domain	151 – 345	195	CheB-type methylesterase
Sites
	Active site	163	1	By similarity
	Active site	190	1	By similarity
	Active site	287	1	By similarity
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TLG9-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 12A03A054F609857	FASTA	349	37,644
	10 20 30 40 50 60 MRTLIVDDSA FMRMAIRSML ASSPDIKIVG DACNGKEAVE KAKSLHPDVV IMDVNMPVMD 70 80 90 100 110 120 GLTAVKTIMN TSPVPIIMFS TLTTEGSKEA LEALHLGAID FTAKSESHHD VNKAEKELVD 130 140 150 160 170 180 KIRNIHSSNP NLLRLINMRK FKGEVVRGKW RCAGDFGILI GSSTGGPSSL EQVIPRLPGD 190 200 210 220 230 240 LPAPVFVVQH MPEGGFCRQM AERLNFLSEL EVKEARNNEK VTAGIVYVAP GGYHMTVRKA 250 260 270 280 290 300 LDVTRIKLIK SQPVHAVMPA VDVTAESMLA VYGKNIVASI LTGMGFDGAS GFKTIRDAGG 310 320 330 340 STIACSEDTC VIFGMPKAAI EAGAIDVVKP IFEIPEEIVK RLEAKCNGK

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM06440.1.
RefSeq	NP_617960.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHD based on UniProtKB P04042.
ModBase	Search...
Genome annotation databases
GeneID	1474961.
GenomeReviews	Gene locus MA_3067 in contig AE010299_GR.
KEGG	mac:MA3067.
NMPDR	fig|188937.1.peg.2986.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TLG9.
OMA	ACSEDTC.
Enzyme and pathway databases
BioCyc	MACE188937:MA3067-MON.
BRENDA	3.1.1.61. 275708.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_METAC

Accession

Primary (citable) accession number: Q8TLG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)