Q8TL49 (Q8TL49_METAC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL AAM06564.1
Ordered Locus Names:	MA_3193

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL AAM06564.1 Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	11	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	252	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	398	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	449	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	452	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	Q8TL49 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E20E9C4374862F88	FASTA	484	53,329
	10 20 30 40 50 60 MHRRSRLKEE CGVAGIILPD DRSQSNTVAF KLYYALYALQ HRGQESTGIM VHDGTSPLSI 70 80 90 100 110 120 KGMGLVPDVY NKDSLGRLIG NAGVGHVRYS TTGGSRIENC QPFILNFKGG TVAIAHNGNL 130 140 150 160 170 180 VNARALKDEL ENEGRIFISN SDTEVIGHLL VKELIRHDPI ESIRNVMRKL VGSYSLVIFI 190 200 210 220 230 240 NGVVYAVRDP FGLKPLCFGE VDGGYGIFSE SVAIDTLNGT LIRDVRPGEV MVFTSSGFES 250 260 270 280 290 300 HQLTNEPHPA HCVFEFIYFA RPDSVIDGKL VYKIRERIGR ELAREHDVEA DIVSPVPDSG 310 320 330 340 350 360 ITSAIGYARE SGIMYLEGLM KNRYIGRTFI LPGQDLRETA VRLKMNAIQE NVKGKRVVLV 370 380 390 400 410 420 DDSIVRGTTS RRIIDMVRKA GASEVHARVG SPAIIAPCYL GIDMATRQEL IASYKTIKEV 430 440 450 460 470 480 EGLINADSLG YLSIEGLMRA LECDKNDMCI GCLTGEYPVD IPGEKCRKKQ SRLDDFSKDA SSSK

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06564.1.
RefSeq	NP_618084.1. NC_003552.1.
3D structure databases
HSSP	HSSP built from PDB template 1AO0 based on UniProtKB P00497.
ProteinModelPortal	Q8TL49.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475086.
GenomeReviews	Gene locus MA_3193 in contig AE010299_GR.
KEGG	mac:MA3193.
NMPDR	fig|188937.1.peg.3110.
Phylogenomic databases
HOGENOM	HBG392416.
OMA	VWAPGEE.
ProtClustDB	CLSK811961.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 2 hits. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8TL49_METAC

Accession

Primary (citable) accession number: Q8TL49

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)