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Protein

Ulilysin

Gene

MA_3214

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloprotease which in vitro specifically cleaves IGFBP-2 to -6, insulin, and extracellular matrix proteins but not IGFBP-1 or IGF-II. Shows a preference for substrates with an arginine in the P1' position, the first position downstream of the scissile bond.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Ca2+1 PublicationNote: Binds 2 calcium ions per subunit.1 Publication
  • Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

Inhibited by EDTA, excess zinc, and also significantly by batimastat in vitro. Is not inhibited by other metalloprotease inhibitors like phosphoramidon, captopril and galardine or those targeting other classes of proteases.2 Publications

pH dependencei

Optimum pH is 7.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225Substrate1 Publication1
Metal bindingi228Zinc; catalyticPROSITE-ProRule annotation2 Publications1
Active sitei229Proton acceptor2 Publications1
Metal bindingi232Zinc; catalyticPROSITE-ProRule annotation2 Publications1
Metal bindingi238Zinc; catalyticPROSITE-ProRule annotation2 Publications1
Metal bindingi240Calcium 1; via carbonyl oxygen2 Publications1
Metal bindingi243Calcium 12 Publications1
Metal bindingi249Calcium 1; via carbonyl oxygen2 Publications1
Metal bindingi254Calcium 22 Publications1
Metal bindingi256Calcium 2; via carbonyl oxygen2 Publications1
Metal bindingi259Calcium 22 Publications1
Metal bindingi262Calcium 1; via carbonyl oxygen2 Publications1
Metal bindingi263Calcium 1; via carbonyl oxygen2 Publications1
Binding sitei295Substrate1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM43.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Ulilysin (EC:3.4.24.-)
Gene namesi
Ordered Locus Names:MA_3214
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000280823? – 60Removed in mature form
Signal peptidei1 – ?Sequence analysis
ChainiPRO_000028082461 – 322UlilysinAdd BLAST262
PropeptideiPRO_0000280825323 – 342Removed in mature formAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi250 ↔ 2772 Publications
Disulfide bondi269 ↔ 297Curated

Post-translational modificationi

The inactive zymogen pro-ulilysin undergoes calcium-mediated autolytic activation to the mature ulilysin. Autoproteolytic activation entails removal of the first 60 residues and of a highly charged 20-residue C-terminal tail.

Keywords - PTMi

Disulfide bond, Zymogen

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

STRINGi188937.MA3214.

Structurei

Secondary structure

1342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi65 – 77Combined sources13
Helixi78 – 80Combined sources3
Helixi84 – 97Combined sources14
Turni98 – 100Combined sources3
Helixi103 – 107Combined sources5
Helixi110 – 115Combined sources6
Beta strandi121 – 125Combined sources5
Beta strandi137 – 142Combined sources6
Helixi153 – 155Combined sources3
Helixi157 – 159Combined sources3
Turni167 – 169Combined sources3
Beta strandi171 – 177Combined sources7
Beta strandi188 – 191Combined sources4
Helixi198 – 200Combined sources3
Beta strandi202 – 206Combined sources5
Helixi207 – 209Combined sources3
Beta strandi210 – 213Combined sources4
Helixi224 – 233Combined sources10
Turni280 – 282Combined sources3
Turni295 – 297Combined sources3
Helixi303 – 314Combined sources12
Turni315 – 317Combined sources3
Helixi318 – 320Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKIX-ray1.70A/B61-322[»]
2J83X-ray2.00A/B61-322[»]
3LUMX-ray1.70A/B/C/D61-322[»]
3LUNX-ray1.80A/B61-322[»]
ProteinModelPortaliQ8TL28.
SMRiQ8TL28.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TL28.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni188 – 189Substrate binding2

Domaini

Consists of two subdomains separated by an active site cleft containing the catalytic zinc ion.

Sequence similaritiesi

Belongs to the peptidase M43B family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410XPH7. LUCA.
InParanoidiQ8TL28.
OMAiRNSFAQP.
PhylomeDBiQ8TL28.

Family and domain databases

CDDicd04275. ZnMc_pappalysin_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR008754. Peptidase_M43.
[Graphical view]
PfamiPF05572. Peptidase_M43. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TL28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKFESRGI EEASSEVPTQ RRCGAMEVHH RLLRSASYVR ERDQIENLAL
60 70 80 90 100
KYKQGFRAIS RMEIVKIPVV VHVVWNEEEE NISDAQIQSQ IDILNKDFRK
110 120 130 140 150
LNSDVSQVPS VWSNLIADLG IEFFLATKDP NGNQTTGITR TQTSVTFFTT
160 170 180 190 200
SDEVKFASSG GEDAWPADRY LNIWVCHVLK SEIGQDILGY AQFPGGPAET
210 220 230 240 250
DGVVIVDAAF GTTGTALPPF DKGRTATHEI GHWLNLYHIW GDELRFEDPC
260 270 280 290 300
SRSDEVDDTP NQADPNFGCP SYPHVSCSNG PNGDMFMNYM DYVDDKCMVM
310 320 330 340
FTQGQATRVN ACLDGPRSSF LARVEETEKK EAPSKREMPM PR
Length:342
Mass (Da):38,371
Last modified:June 1, 2002 - v1
Checksum:iB2480CD7BB2B9574
GO

Mass spectrometryi

Molecular mass is 28885±50 Da from positions 61 - 322. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06585.1.
RefSeqiWP_011023149.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06585; AAM06585; MA_3214.
GeneIDi1475107.
KEGGimac:MA_3214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06585.1.
RefSeqiWP_011023149.1. NC_003552.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKIX-ray1.70A/B61-322[»]
2J83X-ray2.00A/B61-322[»]
3LUMX-ray1.70A/B/C/D61-322[»]
3LUNX-ray1.80A/B61-322[»]
ProteinModelPortaliQ8TL28.
SMRiQ8TL28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA3214.

Protein family/group databases

MEROPSiM43.007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM06585; AAM06585; MA_3214.
GeneIDi1475107.
KEGGimac:MA_3214.

Phylogenomic databases

eggNOGiENOG410XPH7. LUCA.
InParanoidiQ8TL28.
OMAiRNSFAQP.
PhylomeDBiQ8TL28.

Miscellaneous databases

EvolutionaryTraceiQ8TL28.

Family and domain databases

CDDicd04275. ZnMc_pappalysin_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR008754. Peptidase_M43.
[Graphical view]
PfamiPF05572. Peptidase_M43. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiULIL_METAC
AccessioniPrimary (citable) accession number: Q8TL28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.