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Q8TL01

- AMPPA_METAC

UniProt

Q8TL01 - AMPPA_METAC

Protein

AMP phosphorylase

Gene

MA_3242

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.UniRule annotation

    Catalytic activityi

    AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
    CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate.UniRule annotation
    UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei167 – 1671AMP; via amide nitrogenUniRule annotation
    Binding sitei202 – 2021AMP; via amide nitrogenUniRule annotation
    Active sitei255 – 2551Proton donorUniRule annotation
    Binding sitei263 – 2631AMPUniRule annotation
    Binding sitei287 – 2871AMPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi193 – 1986AMPUniRule annotation

    GO - Molecular functioni

    1. AMP binding Source: UniProtKB-HAMAP
    2. phosphorylase activity Source: InterPro
    3. thymidine phosphorylase activity Source: InterPro

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. pyrimidine deoxyribonucleoside metabolic process Source: InterPro
    3. pyrimidine nucleobase metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMACE188937:GI2O-3277-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AMP phosphorylaseUniRule annotation (EC:2.4.2.57UniRule annotation)
    Short name:
    AMPpaseUniRule annotation
    Alternative name(s):
    Nucleoside monophosphate phosphorylaseUniRule annotation
    Short name:
    NMP phosphorylaseUniRule annotation
    Gene namesi
    Ordered Locus Names:MA_3242
    OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
    Taxonomic identifieri188937 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000002487: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506AMP phosphorylasePRO_0000059087Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi188937.MA3242.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TL01.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0213.
    KOiK00758.
    OMAiFINGVRD.
    PhylomeDBiQ8TL01.

    Family and domain databases

    Gene3Di3.40.1030.10. 1 hit.
    3.90.1170.30. 1 hit.
    HAMAPiMF_02132. AMP_phosphorylase.
    InterProiIPR017713. AMP_phosphorylase.
    IPR000312. Glycosyl_Trfase_fam3.
    IPR017459. Glycosyl_Trfase_fam3_N_dom.
    IPR013102. PYNP_C.
    IPR000053. Pyrmidine_PPase.
    IPR017872. Pyrmidine_PPase_CS.
    IPR013466. Thymidine/AMP_Pase.
    [Graphical view]
    PANTHERiPTHR10515. PTHR10515. 1 hit.
    PfamiPF02885. Glycos_trans_3N. 1 hit.
    PF00591. Glycos_transf_3. 1 hit.
    PF07831. PYNP_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000478. TP_PyNP. 1 hit.
    SMARTiSM00941. PYNP_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF47648. SSF47648. 1 hit.
    SSF52418. SSF52418. 1 hit.
    SSF54680. SSF54680. 1 hit.
    TIGRFAMsiTIGR03327. AMP_phos. 1 hit.
    TIGR02645. ARCH_P_rylase. 1 hit.
    PROSITEiPS00647. THYMID_PHOSPHORYLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TL01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLNLEHFDI KIGQHKVMFN VADAKELGVN PGDRVRIRGH QSISAIVDTT    50
    EDMVPPGTLG VFSEVYEHFE DWDKPVEVVP ALRSKSSGVI KKMLDKKSVL 100
    QDEIKLLVND IVEENLSDVE LSAFITASYI HGMTDDEVEW LTRAMIDTGD 150
    TIEFDTHPVM DKHSIGGVPG NKISLLVVPI VAANGLLIPK TSSRAITGAG 200
    GTADLMEVLS PVEFSSEEVK EIAEKVGGAL VWGGATNIAP ADDKLIRVEY 250
    PLSIDPYYQM LASIMAKKGA IGAENVVMDI PIGPSTKVPT VQEGQKLARD 300
    LINLGHRLGM NVECAITYGS SPIGRRVGPC LEVREAMKVL ESMEGPNSLI 350
    EKSAALAGIL LEMGGAAPRD RGKELALETL RNGKALEKMK QIIEAQGGDP 400
    NIKSDDIQVG QYTADIYAST DGYVIEFDNK WIIEIARLAG APNDKGAGVA 450
    IHKKMGEQVK KGDAILTIYA EKEFKLDLAL TTAQRTNPII VEGMLLKRIP 500
    GIYGFQ 506
    Length:506
    Mass (Da):54,862
    Last modified:June 1, 2002 - v1
    Checksum:i17B692D40B346CC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM06613.1.
    RefSeqiNP_618133.1. NC_003552.1.
    WP_011023176.1. NC_003552.1.

    Genome annotation databases

    EnsemblBacteriaiAAM06613; AAM06613; MA_3242.
    GeneIDi1475135.
    KEGGimac:MA3242.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE010299 Genomic DNA. Translation: AAM06613.1 .
    RefSeqi NP_618133.1. NC_003552.1.
    WP_011023176.1. NC_003552.1.

    3D structure databases

    ProteinModelPortali Q8TL01.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 188937.MA3242.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM06613 ; AAM06613 ; MA_3242 .
    GeneIDi 1475135.
    KEGGi mac:MA3242.

    Phylogenomic databases

    eggNOGi COG0213.
    KOi K00758.
    OMAi FINGVRD.
    PhylomeDBi Q8TL01.

    Enzyme and pathway databases

    BioCyci MACE188937:GI2O-3277-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1030.10. 1 hit.
    3.90.1170.30. 1 hit.
    HAMAPi MF_02132. AMP_phosphorylase.
    InterProi IPR017713. AMP_phosphorylase.
    IPR000312. Glycosyl_Trfase_fam3.
    IPR017459. Glycosyl_Trfase_fam3_N_dom.
    IPR013102. PYNP_C.
    IPR000053. Pyrmidine_PPase.
    IPR017872. Pyrmidine_PPase_CS.
    IPR013466. Thymidine/AMP_Pase.
    [Graphical view ]
    PANTHERi PTHR10515. PTHR10515. 1 hit.
    Pfami PF02885. Glycos_trans_3N. 1 hit.
    PF00591. Glycos_transf_3. 1 hit.
    PF07831. PYNP_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000478. TP_PyNP. 1 hit.
    SMARTi SM00941. PYNP_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47648. SSF47648. 1 hit.
    SSF52418. SSF52418. 1 hit.
    SSF54680. SSF54680. 1 hit.
    TIGRFAMsi TIGR03327. AMP_phos. 1 hit.
    TIGR02645. ARCH_P_rylase. 1 hit.
    PROSITEi PS00647. THYMID_PHOSPHORYLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
      Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
      , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
      Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

    Entry informationi

    Entry nameiAMPPA_METAC
    AccessioniPrimary (citable) accession number: Q8TL01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2003
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3