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Q8TL01 (AMPPA_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
AMP phosphorylase

Short name=AMPpase
EC=2.4.2.-
Alternative name(s):
Nucleoside monophosphate phosphorylase
Short name=NMP phosphorylase
Gene names
Ordered Locus Names:MA_3242
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO By similarity. HAMAP-Rule MF_02132

Catalytic activity

AMP + phosphate = D-ribose 1,5-bisphosphate + adenine. HAMAP-Rule MF_02132

CMP + phosphate = D-ribose 1,5-bisphosphate + cytosine. HAMAP-Rule MF_02132

UMP + phosphate = D-ribose 1,5-bisphosphate + uracile. HAMAP-Rule MF_02132

Sequence similarities

Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506AMP phosphorylase HAMAP-Rule MF_02132
PRO_0000059087

Regions

Nucleotide binding193 – 1986AMP By similarity

Sites

Active site2551Proton donor By similarity
Binding site1671AMP; via amide nitrogen By similarity
Binding site2021AMP; via amide nitrogen By similarity
Binding site2631AMP By similarity
Binding site2871AMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TL01 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 17B692D40B346CC5

FASTA50654,862
        10         20         30         40         50         60 
MRLNLEHFDI KIGQHKVMFN VADAKELGVN PGDRVRIRGH QSISAIVDTT EDMVPPGTLG 

        70         80         90        100        110        120 
VFSEVYEHFE DWDKPVEVVP ALRSKSSGVI KKMLDKKSVL QDEIKLLVND IVEENLSDVE 

       130        140        150        160        170        180 
LSAFITASYI HGMTDDEVEW LTRAMIDTGD TIEFDTHPVM DKHSIGGVPG NKISLLVVPI 

       190        200        210        220        230        240 
VAANGLLIPK TSSRAITGAG GTADLMEVLS PVEFSSEEVK EIAEKVGGAL VWGGATNIAP 

       250        260        270        280        290        300 
ADDKLIRVEY PLSIDPYYQM LASIMAKKGA IGAENVVMDI PIGPSTKVPT VQEGQKLARD 

       310        320        330        340        350        360 
LINLGHRLGM NVECAITYGS SPIGRRVGPC LEVREAMKVL ESMEGPNSLI EKSAALAGIL 

       370        380        390        400        410        420 
LEMGGAAPRD RGKELALETL RNGKALEKMK QIIEAQGGDP NIKSDDIQVG QYTADIYAST 

       430        440        450        460        470        480 
DGYVIEFDNK WIIEIARLAG APNDKGAGVA IHKKMGEQVK KGDAILTIYA EKEFKLDLAL 

       490        500 
TTAQRTNPII VEGMLLKRIP GIYGFQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM06613.1.
RefSeqNP_618133.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TL01.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA3242.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM06613; AAM06613; MA_3242.
GeneID1475135.
KEGGmac:MA3242.

Phylogenomic databases

eggNOGCOG0213.
KOK00758.
OMADVWRRMI.
PhylomeDBQ8TL01.
ProtClustDBPRK04350.

Enzyme and pathway databases

BioCycMACE188937:GI2O-3277-MONOMER.

Family and domain databases

Gene3D3.40.1030.10. 1 hit.
3.90.1170.30. 1 hit.
HAMAPMF_02132. AMP_phosphorylase.
InterProIPR017713. AMP_phosphorylase.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERPTHR10515. PTHR10515. 1 hit.
PfamPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
PIRSFPIRSF000478. TP_PyNP. 1 hit.
SMARTSM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsTIGR03327. AMP_phos. 1 hit.
TIGR02645. ARCH_P_rylase. 1 hit.
PROSITEPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPPA_METAC
AccessionPrimary (citable) accession number: Q8TL01
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families