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Protein

Probable tRNA pseudouridine synthase D

Gene

truD

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(13) = tRNA pseudouridine(13).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891NucleophileUniRule annotation

GO - Molecular functioni

  1. pseudouridine synthase activity Source: UniProtKB-HAMAP
  2. RNA binding Source: InterPro

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3306-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase DUniRule annotation (EC:5.4.99.27UniRule annotation)
Alternative name(s):
tRNA pseudouridine(13) synthaseUniRule annotation
tRNA pseudouridylate synthase DUniRule annotation
tRNA-uridine isomerase DUniRule annotation
Gene namesi
Name:truDUniRule annotation
Ordered Locus Names:MA_3271
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Probable tRNA pseudouridine synthase DPRO_0000152539Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi188937.MA3271.

Structurei

3D structure databases

ProteinModelPortaliQ8TKX2.
SMRiQ8TKX2. Positions 4-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 393226TRUDUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pseudouridine synthase TruD family.UniRule annotation
Contains 1 TRUD domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0585.
InParanoidiQ8TKX2.
KOiK06176.
OMAiLRYERAM.
PhylomeDBiQ8TKX2.

Family and domain databases

HAMAPiMF_01082. TruD.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001656. PsdUridine_synth_TruD.
IPR020119. PsdUridine_synth_TruD_CS.
IPR011760. PsdUridine_synth_TruD_insert.
[Graphical view]
PANTHERiPTHR13326. PTHR13326. 1 hit.
PfamiPF01142. TruD. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00094. tRNA_TruD_broad. 1 hit.
PROSITEiPS50984. TRUD. 1 hit.
PS01268. UPF0024. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TKX2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPMEVPEIE KQIGINLYST NTEGLGGRLR QEVEDFIVKE ITNREEGKDG
60 70 80 90 100
RYLVLELTKR DWDTHHFTRT LAKILQISQK RISVAGTKDK RALTTQKISI
110 120 130 140 150
FDIDALEIEK IHLKDVELKV LGRSRKSVEL GDLWGNEFII TIRDISSSPE
160 170 180 190 200
ETRTILEKTN SKVLTQGGVP NFFGVQRFGS VRSVTHLVGK AIVEGNFEKA
210 220 230 240 250
AMLYIAEPFP EEPEETKAAR QFVKETRDFK EGLKTYPLRL GHERAMMNHL
260 270 280 290 300
ISNPEDYSGA FSVLPKNLYR MFVHAYQSYI YNMILCRRIE SGISLNRAVE
310 320 330 340 350
GDIVCFRNEA GLPDSSKTEK VTSETVNAMN RLIKHGRAFI TAPLPGFNTE
360 370 380 390 400
FASGVPGEIE SKILKELRVS LEGFNVEEFP EMSSKGTRRE VLLQVEPKFE
410 420 430 440
VGEDELNPGK SKTVLEFMLP KGSYATTVLR EYMKVDPLQM S
Length:441
Mass (Da):50,058
Last modified:June 1, 2002 - v1
Checksum:iA3A401EA4D900365
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06642.1.
RefSeqiNP_618162.1. NC_003552.1.
WP_011023205.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06642; AAM06642; MA_3271.
GeneIDi1475164.
KEGGimac:MA3271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06642.1.
RefSeqiNP_618162.1. NC_003552.1.
WP_011023205.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TKX2.
SMRiQ8TKX2. Positions 4-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA3271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM06642; AAM06642; MA_3271.
GeneIDi1475164.
KEGGimac:MA3271.

Phylogenomic databases

eggNOGiCOG0585.
InParanoidiQ8TKX2.
KOiK06176.
OMAiLRYERAM.
PhylomeDBiQ8TKX2.

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3306-MONOMER.

Family and domain databases

HAMAPiMF_01082. TruD.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001656. PsdUridine_synth_TruD.
IPR020119. PsdUridine_synth_TruD_CS.
IPR011760. PsdUridine_synth_TruD_insert.
[Graphical view]
PANTHERiPTHR13326. PTHR13326. 1 hit.
PfamiPF01142. TruD. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 2 hits.
TIGRFAMsiTIGR00094. tRNA_TruD_broad. 1 hit.
PROSITEiPS50984. TRUD. 1 hit.
PS01268. UPF0024. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiTRUD_METAC
AccessioniPrimary (citable) accession number: Q8TKX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2002
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.