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Q8TKT6 (PUR2_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:MA_3309
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151509

Regions

Domain112 – 319208ATP-grasp
Nucleotide binding139 – 19658ATP By similarity

Sites

Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 1 By similarity
Metal binding2891Magnesium or manganese 2 By similarity
Metal binding2911Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TKT6 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: CDC8AF5A3B9C4C9C

FASTA44147,750
        10         20         30         40         50         60 
MKMKILLIGG GGREHAIAEG IKESKHNPIL YALMAKKNPG IAALCEDFLL EKETEVEKIV 

        70         80         90        100        110        120 
EYAKARNIEM AFVGPEAPLA AGVADALWEA GIPVVGPKKA CAVIEFDKAW ARNFMKKYGI 

       130        140        150        160        170        180 
EGCPAYEVFT EEAPAHAFIE KLGDVAVKPS GLTGGKGVKV MGDQLPDLKA AKDYTSELLE 

       190        200        210        220        230        240 
KGPVVIEERF IGEEFTLQAF VDGKNLAFFP AVQDHKRAYE GDLGPNTGGM GSYTDAGEIL 

       250        260        270        280        290        300 
PFMLAGDIEQ AKKIMQHTVT ALHEETGTGY QGVLYGQFIL TASGPKVVEF NARFGDPEAM 

       310        320        330        340        350        360 
NVIPLIETDF TEIMSAVVKS TLDSLPVKFS RKATVCKYAV PAGYPDNPEK DSEVLVGDVG 

       370        380        390        400        410        420 
EASVYYASVY EKEGKIYTTG SRAVAVVGRA ETIDAAEKIA QNALENIQGK LFFRKDIGTA 

       430        440 
SLIQKRIDHM KELRGGLSQK Q 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM06679.1.
RefSeqNP_618199.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TKT6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA3309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM06679; AAM06679; MA_3309.
GeneID1475202.
KEGGmac:MA3309.

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMADPEAMNV.
PhylomeDBQ8TKT6.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycMACE188937:GI2O-3350-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METAC
AccessionPrimary (citable) accession number: Q8TKT6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways