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Protein

UvrABC system protein B

Gene

uvrB

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 718ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. excinuclease ABC activity Source: UniProtKB-HAMAP
  4. helicase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nucleotide-excision repair Source: UniProtKB-HAMAP
  2. SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3365-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:MA_3323
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 670670UvrABC system protein BPRO_0000138454Add
BLAST

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA3323.

Structurei

3D structure databases

ProteinModelPortaliQ8TKS3.
SMRiQ8TKS3. Positions 29-614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 433383Helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini453 – 612160Helicase C-terminalUniRule annotationAdd
BLAST
Domaini631 – 66636UVRUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi117 – 14024Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation
Contains 1 helicase ATP-binding domain.UniRule annotation
Contains 1 helicase C-terminal domain.UniRule annotation
Contains 1 UVR domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0556.
InParanoidiQ8TKS3.
KOiK03702.
OMAiNKTLCAQ.
PhylomeDBiQ8TKS3.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TKS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSSQTPGE LSKKKFELLH DARYWDSPQF KLISGFEPKG SQPQAIEKLV
60 70 80 90 100
EGLKKREQFQ TLLGVTGSGK TYTVANVINQ IRKPTLVIAH NKTLAAQLYN
110 120 130 140 150
EFREFFPENR VEYFVSYYDY YQPESYLPAK DQYIEKDAQI NPKIEQMRLA
160 170 180 190 200
ATASLMSRQD VIVVASVSCI YGLGNPENFQ KMGFELKVGD KVQRKEILEK
210 220 230 240 250
LIDIQFERND MELMPGRFRV KGDTIDIIPG YFDDIIRVEL FGDEVDRISE
260 270 280 290 300
VDKQTGQRKE DMDYFFVYPA RHYVIPEEEQ KSAIRSILEE LEEHLPTLGL
310 320 330 340 350
LESHRLKQRT LYDMEMIEET GSCKGIENYS RHFDHRQPGE QPFCLLDYFP
360 370 380 390 400
EDFLLIIDES HQTIPQLHGM YNGDRSRKKS LVDYGFRLPS AYDNRPLKFE
410 420 430 440 450
EFEKYMENVI FVSATPSDYE REHSARIVEQ IIRPTGLVDP EVEVRPLEGQ
460 470 480 490 500
VRDVMQEIRK IVDRGDRALV TTLTKKLAEE LTEFLARNEI KARYLHSDIK
510 520 530 540 550
TIERTEIIRE LRLGKFDVLV GINLLREGLD IPEVGFIGIL DADKEGFLRD
560 570 580 590 600
SKSLIQIIGR AARNSSSKVV LYADNMTESI KKAVDETERR RSMQIAYNEE
610 620 630 640 650
HGIVPKTIRK PIREKVVDIT DTKHIPKTDI PNVIIELDAE MREAADRLDF
660 670
ERAIQLRELI KKLEKEVKAV
Length:670
Mass (Da):77,789
Last modified:May 31, 2002 - v1
Checksum:i4BB8ED22CE9718BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06692.1.
RefSeqiNP_618212.1. NC_003552.1.
WP_011023255.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06692; AAM06692; MA_3323.
GeneIDi1475216.
KEGGimac:MA3323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM06692.1.
RefSeqiNP_618212.1. NC_003552.1.
WP_011023255.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TKS3.
SMRiQ8TKS3. Positions 29-614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA3323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM06692; AAM06692; MA_3323.
GeneIDi1475216.
KEGGimac:MA3323.

Phylogenomic databases

eggNOGiCOG0556.
InParanoidiQ8TKS3.
KOiK03702.
OMAiNKTLCAQ.
PhylomeDBiQ8TKS3.

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3365-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiUVRB_METAC
AccessioniPrimary (citable) accession number: Q8TKS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 12, 2002
Last sequence update: May 31, 2002
Last modified: March 31, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.