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Q8TKS3 (UVRB_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UvrABC system protein B

Short name=Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene names
Name:uvrB
Ordered Locus Names:MA_3323
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length670 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 UVR domain.

Ontologies

Keywords
   Biological processDNA damage
DNA excision
DNA repair
SOS response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionExcision nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSOS response

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleotide-excision repair

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

excinuclease ABC activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 670670UvrABC system protein B HAMAP-Rule MF_00204
PRO_0000138454

Regions

Domain51 – 433383Helicase ATP-binding
Domain453 – 612160Helicase C-terminal
Domain631 – 66636UVR
Nucleotide binding64 – 718ATP Potential
Motif117 – 14024Beta-hairpin HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
Q8TKS3 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 4BB8ED22CE9718BB

FASTA67077,789
        10         20         30         40         50         60 
MKVSSQTPGE LSKKKFELLH DARYWDSPQF KLISGFEPKG SQPQAIEKLV EGLKKREQFQ 

        70         80         90        100        110        120 
TLLGVTGSGK TYTVANVINQ IRKPTLVIAH NKTLAAQLYN EFREFFPENR VEYFVSYYDY 

       130        140        150        160        170        180 
YQPESYLPAK DQYIEKDAQI NPKIEQMRLA ATASLMSRQD VIVVASVSCI YGLGNPENFQ 

       190        200        210        220        230        240 
KMGFELKVGD KVQRKEILEK LIDIQFERND MELMPGRFRV KGDTIDIIPG YFDDIIRVEL 

       250        260        270        280        290        300 
FGDEVDRISE VDKQTGQRKE DMDYFFVYPA RHYVIPEEEQ KSAIRSILEE LEEHLPTLGL 

       310        320        330        340        350        360 
LESHRLKQRT LYDMEMIEET GSCKGIENYS RHFDHRQPGE QPFCLLDYFP EDFLLIIDES 

       370        380        390        400        410        420 
HQTIPQLHGM YNGDRSRKKS LVDYGFRLPS AYDNRPLKFE EFEKYMENVI FVSATPSDYE 

       430        440        450        460        470        480 
REHSARIVEQ IIRPTGLVDP EVEVRPLEGQ VRDVMQEIRK IVDRGDRALV TTLTKKLAEE 

       490        500        510        520        530        540 
LTEFLARNEI KARYLHSDIK TIERTEIIRE LRLGKFDVLV GINLLREGLD IPEVGFIGIL 

       550        560        570        580        590        600 
DADKEGFLRD SKSLIQIIGR AARNSSSKVV LYADNMTESI KKAVDETERR RSMQIAYNEE 

       610        620        630        640        650        660 
HGIVPKTIRK PIREKVVDIT DTKHIPKTDI PNVIIELDAE MREAADRLDF ERAIQLRELI 

       670 
KKLEKEVKAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM06692.1.
RefSeqNP_618212.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TKS3.
SMRQ8TKS3. Positions 29-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA3323.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM06692; AAM06692; MA_3323.
GeneID1475216.
KEGGmac:MA3323.

Phylogenomic databases

eggNOGCOG0556.
KOK03702.
OMALEKQMHE.
PhylomeDBQ8TKS3.

Enzyme and pathway databases

BioCycMACE188937:GI2O-3365-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUVRB_METAC
AccessionPrimary (citable) accession number: Q8TKS3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families