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Q8TKQ5 (NADK_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:MA_3343
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275NAD kinase HAMAP-Rule MF_00361
PRO_0000120699

Regions

Nucleotide binding68 – 692NAD By similarity
Nucleotide binding136 – 1372NAD By similarity
Nucleotide binding177 – 1826NAD By similarity

Sites

Active site681Proton acceptor By similarity
Binding site731NAD By similarity
Binding site1471NAD By similarity
Binding site1641NAD By similarity
Binding site1661NAD By similarity
Binding site2011NAD; via carbonyl oxygen By similarity
Binding site2361NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TKQ5 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 1B45C07917BB5CED

FASTA27530,499
        10         20         30         40         50         60 
MAIRKIGIAS RCDRPEVLQM VRDIIAHFYS KVQIYVSTAT ADVLDIEGTP VERMRDKGVE 

        70         80         90        100        110        120 
LIISVGGDGT VLRNIAKMKD PLPVLGINMG TLGFLVDVEP EDAIETIEEV LYGFSYLERM 

       130        140        150        160        170        180 
RVDVFLNGEM LETATNEVAV MSAKPAKIIQ FEVYVNDCLL DEMRADGVVF ATPTGSTAYA 

       190        200        210        220        230        240 
MSAGGPIINP RVNAIVVVPV APFKLSARPW VIPSDSEITV KLSDHKKEAV IAIDGQKSYR 

       250        260        270 
IRPDDVVKLK KSKYPARFVR ISDTCFYERV QRKLS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM06712.1.
RefSeqNP_618232.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TKQ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA3343.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM06712; AAM06712; MA_3343.
GeneID1475236.
KEGGmac:MA3343.

Phylogenomic databases

eggNOGCOG0061.
KOK00858.
OMATHEMLYH.
PhylomeDBQ8TKQ5.

Enzyme and pathway databases

BioCycMACE188937:GI2O-3385-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_METAC
AccessionPrimary (citable) accession number: Q8TKQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: June 1, 2002
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families