Q8TKQ5 (PPNK_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	MA_3343

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity. HAMAP MF_00361
Subcellular location	Cytoplasm By similarity HAMAP MF_00361.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_0000120699

Sequences

	Sequence		Length	Mass (Da)
	Q8TKQ5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1B45C07917BB5CED	FASTA	275	30,499
	10 20 30 40 50 60 MAIRKIGIAS RCDRPEVLQM VRDIIAHFYS KVQIYVSTAT ADVLDIEGTP VERMRDKGVE 70 80 90 100 110 120 LIISVGGDGT VLRNIAKMKD PLPVLGINMG TLGFLVDVEP EDAIETIEEV LYGFSYLERM 130 140 150 160 170 180 RVDVFLNGEM LETATNEVAV MSAKPAKIIQ FEVYVNDCLL DEMRADGVVF ATPTGSTAYA 190 200 210 220 230 240 MSAGGPIINP RVNAIVVVPV APFKLSARPW VIPSDSEITV KLSDHKKEAV IAIDGQKSYR 250 260 270 IRPDDVVKLK KSKYPARFVR ISDTCFYERV QRKLS

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06712.1.
RefSeq	NP_618232.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TKQ5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475236.
GenomeReviews	Gene locus MA_3343 in contig AE010299_GR.
KEGG	mac:MA3343.
NMPDR	fig|188937.1.peg.3258.
Phylogenomic databases
HOGENOM	HBG713904.
OMA	GPVVWPE.
ProtClustDB	CLSK568985.
Enzyme and pathway databases
BioCyc	MACE188937:MA3343-MONOMER.
Family and domain databases
HAMAP	MF_00361. NAD_kinase. [Tree]
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
KO	K00858.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPNK_METAC

Accession

Primary (citable) accession number: Q8TKQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program