Q8TKM8 (ILVD2_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2
Short name=DAD 2
EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	MA_3373

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 553	553	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103540
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TKM8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 72B85ACC5C1AF1FA	FASTA	553	58,689
	10 20 30 40 50 60 MRSDIIKEGP ERAPNRSLLK ATGVTDSEMK KPFIAVVNSW NDIIPGHMHL NKLAEAVKAG 70 80 90 100 110 120 IRNAGGVPFE FHTVGVCDGI AMGHEGMKYS LPSREIIEDT IELMVKAHQF DGMVLIPSCD 130 140 150 160 170 180 KIVPGHLMAA GRLDIPAIVV TGGPMLPGYV DDNYRDLISV SEGVGAYSAG KISETEFKRL 190 200 210 220 230 240 ENLSCAGAGS CAGMFTANTM ACMTEALGLS LPGCATAHAV DAKKILIAKE SGERIVELVK 250 260 270 280 290 300 ENLTPRKIVT QKSFENAIMV DMAVGGSTNT TLHLPALAHE FGLGLPLEAF DELSRKTPHL 310 320 330 340 350 360 ISLRPGGPNF ILHFDRAGGV EAVMQRLASK LHLDQLTVNG KTIGENLDEL EIVNPKLNAE 370 380 390 400 410 420 IITTLENPIH AEGGIAVLKG SLAPDGSVVK QAAVDPKMHV YTGPAKVYDC EEDAMKSILA 430 440 450 460 470 480 GDVKPGDIVV IRYEGPKGGP GMREMLAATA ALGGRGLLES VALVTDGRFS GGTRGPCIGH 490 500 510 520 530 540 VSPEASDGGP IALVKDGDLI EINIPERALN LKISEEELKQ RKASFVPPKT EITGYLARYQ 550 RAVHSANTGG IVD

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06742.1.
RefSeq	NP_618262.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475266.
GenomeReviews	Gene locus MA_3373 in contig AE010299_GR.
KEGG	mac:MA3373.
NMPDR	fig|188937.1.peg.3288.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NIPERAL.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MACE188937:MA3373-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_METAC

Accession

Primary (citable) accession number: Q8TKM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program