Q8TK72 (PGP_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycolate phosphatase
Short name=PGP
Short name=PGPase
EC=3.1.3.18

Gene names

Ordered Locus Names:

MA_3544

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of 2-phosphoglycolate By similarity. HAMAP MF_01419
Catalytic activity	2-phosphoglycolate + H2O = glycolate + phosphate. HAMAP MF_01419
Cofactor	Magnesium By similarity. HAMAP MF_01419
Sequence similarities	Belongs to the archaeal SPP-like hydrolase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Phosphoglycolate phosphatase HAMAP MF_01419		PRO_0000146717
Sites
	Active site	9	1	Nucleophile By similarity
	Metal binding	9	1	Magnesium By similarity
	Metal binding	11	1	Magnesium By similarity
	Metal binding	173	1	Magnesium By similarity
	Metal binding	177	1	Magnesium By similarity
	Binding site	150	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TK72 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: AC2D2C34F52C5498	FASTA	226	25,006
	10 20 30 40 50 60 MKFKAIVIDI DGTITCENRE LHLGAVKKIR ILKVPVVLAT GNILCYARTA SKLIGLDGAV 70 80 90 100 110 120 IAENGGAVTV RYDLNGTFEE SLEECEKAFS FLSEYFKLTK LDPFYRKTEI ALRRDFNLEK 130 140 150 160 170 180 ARTLLETQPF DVEMVDTKYA VHIKSTRINK GSGLRKLAEI MGLEAEDFVA IGDSENDIEM 190 200 210 220 FEAAGFGIAV ANGDERIKEA ANYVTEASYG DGAVEAIEFL ESNGWI

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06906.1.
RefSeq	NP_618426.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TK72.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475437.
GenomeReviews	Gene locus MA_3544 in contig AE010299_GR.
KEGG	mac:MA3544.
NMPDR	fig|188937.1.peg.3452.
Phylogenomic databases
HOGENOM	HBG551920.
OMA	RESTRID.
ProtClustDB	PRK01158.
Enzyme and pathway databases
BioCyc	MACE188937:MA3544-MONOMER.
Family and domain databases
HAMAP	MF_01419. GPH_hydrolase_arch. [Tree]
InterPro	IPR023214. HAD-like_dom. IPR013200. HAD-SF_hydro-like_3. IPR006379. HAD-SF_hydro_IIB. IPR006382. SPPlik_hydro_arc. IPR006378. Suc_phosP. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K07024.
Pfam	PF08282. Hydrolase_3. 2 hits. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01484. HAD-SF-IIB. 1 hit. TIGR01487. SPP-like. 1 hit. TIGR01482. SPP-subfamily. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PGP_METAC

Accession

Primary (citable) accession number: Q8TK72

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program