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Q8TK55 (ARGJ_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Arginine biosynthesis bifunctional protein ArgJ

Cleaved into the following 2 chains:

  1. Arginine biosynthesis bifunctional protein ArgJ alpha chain
  2. Arginine biosynthesis bifunctional protein ArgJ beta chain

Including the following 2 domains:

  1. Glutamate N-acetyltransferase
    EC=2.3.1.35
    Alternative name(s):
    Ornithine acetyltransferase
    Short name=OATase
    Ornithine transacetylase
  2. Amino-acid acetyltransferase
    EC=2.3.1.1
    Alternative name(s):
    N-acetylglutamate synthase
    Short name=AGS
Gene names
Name:argJ
Ordered Locus Names:MA_3564
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106

Catalytic activity

N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106

Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106

Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106

Subunit structure

Heterotetramer of two alpha and two beta chains By similarity.

Subcellular location

Cytoplasm Probable HAMAP MF_01106.

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106

Sequence similarities

Belongs to the ArgJ family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Arginine biosynthesis bifunctional protein ArgJ alpha chain By similarity
PRO_0000002271
Chain180 – 395216Arginine biosynthesis bifunctional protein ArgJ beta chain By similarity
PRO_0000002272

Sequences

Sequence LengthMass (Da)Tools
Q8TK55 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 3CF204E113213F44

FASTA39541,700
        10         20         30         40         50         60 
MKKIEGGICA VKGVTANGIK LGKMGITVIR AEGPAAGVFT KNKVTAAPVV LSKGVIETQH 

        70         80         90        100        110        120 
QLSAIIANSG NANAFTGDDG FLDAMEMASA LSESLDLEPD TVAVASTGVI GRRLDVSWIR 

       130        140        150        160        170        180 
EHLPEVLEGL GSSPECSLAA AKAIMTTDKA LKEVAVELDC GVRIGAIAKG SGMIEPNMGT 

       190        200        210        220        230        240 
MLCFAYTDAL VPADVLDAAL RIAVDKTFNM VVVDGDTSTN DMVLFTSTCK SGIKPCMECL 

       250        260        270        280        290        300 
DEFEDALIYL FTDLAKKMAR DGEGATKLIE ARVTGAKTYE DARLAVKAIV RSPLVKSAIF 

       310        320        330        340        350        360 
GKDPNWGRVV AAAGYSGAEL EQERLSLSFS AGGETVELVK SGEISRVSDL ALLNKIMANE 

       370        380        390 
EIIITLDFGM GKESATAWGC DLTYDYVRIN AEYTT

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE010299 Genomic DNA. Translation: AAM06925.1.
RefSeqNP_618445.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TK55.
ModBaseSearch...

Protein family/group databases

MEROPST05.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1475457.
GenomeReviewsGene locus MA_3564 in contig AE010299_GR.
KEGGmac:MA3564.
NMPDRfig|188937.1.peg.3471.

Phylogenomic databases

HOGENOMHBG284202.
OMAGRDPNWG.
ProtClustDBPRK05388.

Enzyme and pathway databases

BioCycMACE188937:MA3564-MONOMER.

Family and domain databases

HAMAPMF_01106. ArgJ. Divergent sequence.
[Tree]
InterProIPR002813. Arg_biosynth_ArgJ.
IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ.
[Graphical view]
KOK00620.
PANTHERPTHR23100. ArgJ. 1 hit.
PfamPF01960. ArgJ. 1 hit.
[Graphical view]
ProDomPD004193. Arg_biosynth_ArgJ. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMsTIGR00120. ArgJ. 1 hit.
ProtoNetSearch...

Entry information

Entry nameARGJ_METAC
AccessionPrimary (citable) accession number: Q8TK55
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: June 1, 2002
Last modified: November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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