Reviewed, UniProtKB/Swiss-Prot Q8TK32 (PGK2_METAC)

Names and origin

Protein names

Recommended name:
    Phosphoglycerate kinase 2
    EC=2.7.2.3

Gene names

Name:	pgk2
Ordered Locus Names:	MA_3592

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Phosphoglycerate kinase 2 HAMAP MF_00145		PRO_0000146055
Regions
	Nucleotide binding	362 – 365	4	ATP By similarity
	Region	28 – 30	3	Substrate binding By similarity
	Region	65 – 68	4	Substrate binding By similarity
Sites
	Binding site	44	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TK32-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: AF59939C97C156AF	FASTA	416	45,499
	10 20 30 40 50 60 MLRVMTSRNF LTIDDFDIRG KTILLRVDMN SPMDTQGHIL DDMRIKSHIA TLKDLESAKV 70 80 90 100 110 120 VLLAHQSRPG KKDFTTMKPH AHLLSRYLGK QVTYVDDIFG TFAKTHIASM EDGDVIMLEN 130 140 150 160 170 180 VRFYSEESLE RTPAEQANTY MVKKLAPFVD IFLNDAFAVA HRSHLSVVGF TEVLPSGAGR 190 200 210 220 230 240 VMEKELVSLD RGVKGGERPS IFVLGGAKVD DSLRVTENVL TSGGADRVLL TGVVANVALA 250 260 270 280 290 300 ASGVNIGKVN MDFIKSQGYE NQIEKARGLL AKFKDRIGLP KDVALNDNRE RVEVHISELN 310 320 330 340 350 360 SDSLPINDIG LETIVDYTNE IQNSKTVVLN GPAGVSEIED FALGTHEIIK AAIKSDFSII 370 380 390 400 410 GGGHISVEVA HLGLEHRFSH ISTGGGACID YLAGEKLPGV ESLKAAYIKY QEAKKL

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM06948.1.
RefSeq	NP_618468.1.
3D structure databases
HSSP	HSSP built from PDB template 1VPE based on UniProtKB P36204.
ModBase	Search...
Genome annotation databases
GeneID	1475485.
GenomeReviews	Gene locus MA_3592 in contig AE010299_GR.
KEGG	mac:MA3592.
NMPDR	fig|188937.1.peg.3494.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TK32.
OMA	PGKKDFT.
Enzyme and pathway databases
BioCyc	MACE188937:MA3592-MON.
BRENDA	2.7.2.3. 275708.
Family and domain databases
HAMAP	MF_00145. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK2_METAC

Accession

Primary (citable) accession number: Q8TK32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)