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Q8TJS2

- KAE1B_METAC

UniProt

Q8TJS2 - KAE1B_METAC

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Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
MA_3705
Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Iron By similarity
Metal bindingi117 – 1171Iron By similarity
Metal bindingi134 – 1341Iron By similarity
Binding sitei166 – 1661Threonylcarbamoyl-AMP By similarity
Binding sitei179 – 1791Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei183 – 1831Threonylcarbamoyl-AMP By similarity
Binding sitei262 – 2621Threonylcarbamoyl-AMP By similarity
Metal bindingi290 – 2901Iron By similarity
Binding sitei377 – 3771ATP By similarity
Active sitei464 – 4641Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3639ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  6. protein tyrosine kinase activity Source: InterPro
  7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:MA_3705
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 547547Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
PRO_0000303654Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi188937.MA3705.

Structurei

3D structure databases

ProteinModelPortaliQ8TJS2.
SMRiQ8TJS2. Positions 5-330.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini340 – 547208Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 329329Kae1UniRule annotation
Add
BLAST
Regioni134 – 1385Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
KOiK15904.
OMAiRDNAGMI.
PhylomeDBiQ8TJS2.

Family and domain databases

HAMAPiMF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TJS2-1 [UniParc]FASTAAdd to Basket

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MKNTFILGIE GTAWNLSAAI VTETEIIAEV TETYKPEVGG IHPREAAQHH    50
AKYAASVIKR LLAEAKEKGV EPSDLDGIAF SQGPGLGPCL RTIATAARML 100
SLSLDIPLIG VNHCIAHIEI GIWRTPARDP VVLYVSGANS QVISFMEGRY 150
RVFGETLDIG LGNALDKFAR RAGLPHPGGP KIEACAKDAK RYIPLPYVIK 200
GMDLSFSGLS TASSEALKKA SLEDVCYSYQ ETAFAMVVEV AERALAHTGK 250
NEVLLAGGVG ANTRLREMLN EMCEARGAKF YVPEKRFMGD NGTMIAYTGL 300
LMYKSGNTLT LEDSRVNPNF RTDDVNVTWI KEEEMKKVPE ISPEAFLRAP 350
PGERLDNGAE AVIYLDEGPE GKKVLVKERV PKLYRHKEID ERIRRERNRT 400
EARLISEARR AGVPTPIIYD IEEFKLKMQF IEGVPIKYLI TPELSEKVGE 450
LVGRLHSSGI VHGDLTTSNL LLAGERLYLI DFGLAYFDKS LEARGVDVHV 500
LFQTFESTHR GHETLVKAFE KGYGSTFIDS KDVLKRVEEI KKRARYA 547
Length:547
Mass (Da):60,639
Last modified:June 1, 2002 - v1
Checksum:iCE1798004A897139
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM07060.1.
RefSeqiNP_618580.1. NC_003552.1.
WP_011023612.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM07060; AAM07060; MA_3705.
GeneIDi1475598.
KEGGimac:MA3705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM07060.1 .
RefSeqi NP_618580.1. NC_003552.1.
WP_011023612.1. NC_003552.1.

3D structure databases

ProteinModelPortali Q8TJS2.
SMRi Q8TJS2. Positions 5-330.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 188937.MA3705.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM07060 ; AAM07060 ; MA_3705 .
GeneIDi 1475598.
KEGGi mac:MA3705.

Phylogenomic databases

eggNOGi COG0533.
KOi K15904.
OMAi RDNAGMI.
PhylomeDBi Q8TJS2.

Enzyme and pathway databases

BioCyci MACE188937:GI2O-3748-MONOMER.

Family and domain databases

HAMAPi MF_01446. Kae1_arch.
MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO-like_kinase.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
PF01163. RIO1. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiKAE1B_METAC
AccessioniPrimary (citable) accession number: Q8TJS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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