Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5,10-methylenetetrahydromethanopterin reductase

Gene

mer

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT.UniRule annotation

Catalytic activityi

5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Source: UniProtKB-HAMAP
  2. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Source: InterPro

GO - Biological processi

  1. methanogenesis, from carbon dioxide Source: UniProtKB-UniPathway
  2. one-carbon metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methanogenesis, One-carbon metabolism

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3776-MONOMER.
UniPathwayiUPA00640; UER00697.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydromethanopterin reductaseUniRule annotation (EC:1.5.98.2UniRule annotation)
Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductaseUniRule annotation
Methylene-H(4)MPT reductaseUniRule annotation
Gene namesi
Name:merUniRule annotation
Ordered Locus Names:MA_3733
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3283285,10-methylenetetrahydromethanopterin reductasePRO_0000084806Add
BLAST

Proteomic databases

PRIDEiQ8TJP7.

Interactioni

Protein-protein interaction databases

STRINGi188937.MA3733.

Structurei

3D structure databases

ProteinModelPortaliQ8TJP7.
SMRiQ8TJP7. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mer family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2141.
InParanoidiQ8TJP7.
KOiK00320.
OMAiPALKIAY.
PhylomeDBiQ8TJP7.

Family and domain databases

Gene3Di3.20.20.30. 1 hit.
HAMAPiMF_01091. F420_mer.
InterProiIPR011251. Luciferase-like_dom.
IPR019946. MeH4methanopterin_reductase.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
SUPFAMiSSF51679. SSF51679. 1 hit.
TIGRFAMsiTIGR03555. F420_mer. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TJP7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFGIEFVPS DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA
60 70 80 90 100
LNTNSIKIGS GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF
110 120 130 140 150
DAMGIAWEKP LATTKEAIQA IRDFIAGKKV SMDGEMVKFA GAKLAFKAGN
160 170 180 190 200
VPIYMGAQGP KMLELAGEVA DGVLINASHP KDFEVAVEQI RKGAEKVGRD
210 220 230 240 250
PSEVDVTAYA CFSIDKDPAK AINAAKVVVA FIVAGSPDLV LERHGIPVDA
260 270 280 290 300
KKQIGDAIAK GDFGALMGGL VTPQMIEAFA ICGTPEDCMK RIKDLEAIGV
310 320
TQIVAGSPIG PEKEKAIKLI GKEIIAKM
Length:328
Mass (Da):34,720
Last modified:June 1, 2002 - v1
Checksum:iC57ABD6B163CBD4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM07085.1.
RefSeqiNP_618605.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM07085; AAM07085; MA_3733.
GeneIDi1475626.
KEGGimac:MA3733.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM07085.1.
RefSeqiNP_618605.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TJP7.
SMRiQ8TJP7. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA3733.

Proteomic databases

PRIDEiQ8TJP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM07085; AAM07085; MA_3733.
GeneIDi1475626.
KEGGimac:MA3733.

Phylogenomic databases

eggNOGiCOG2141.
InParanoidiQ8TJP7.
KOiK00320.
OMAiPALKIAY.
PhylomeDBiQ8TJP7.

Enzyme and pathway databases

UniPathwayiUPA00640; UER00697.
BioCyciMACE188937:GI2O-3776-MONOMER.

Family and domain databases

Gene3Di3.20.20.30. 1 hit.
HAMAPiMF_01091. F420_mer.
InterProiIPR011251. Luciferase-like_dom.
IPR019946. MeH4methanopterin_reductase.
[Graphical view]
PfamiPF00296. Bac_luciferase. 1 hit.
[Graphical view]
SUPFAMiSSF51679. SSF51679. 1 hit.
TIGRFAMsiTIGR03555. F420_mer. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiMER_METAC
AccessioniPrimary (citable) accession number: Q8TJP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: June 1, 2002
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.