Q8TJP7 (MER_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MA_3733

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP-Rule MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP-Rule MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP-Rule MF_01091
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methanogenesis, from carbon dioxide Inferred from electronic annotation. Source: UniProtKB-UniPathway one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	5,10-methylenetetrahydromethanopterin reductase HAMAP-Rule MF_01091		PRO_0000084806

Sequences

	Sequence		Length	Mass (Da)
	Q8TJP7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C57ABD6B163CBD4E	FASTA	328	34,720
	10 20 30 40 50 60 MKFGIEFVPS DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA LNTNSIKIGS 70 80 90 100 110 120 GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWEKP LATTKEAIQA 130 140 150 160 170 180 IRDFIAGKKV SMDGEMVKFA GAKLAFKAGN VPIYMGAQGP KMLELAGEVA DGVLINASHP 190 200 210 220 230 240 KDFEVAVEQI RKGAEKVGRD PSEVDVTAYA CFSIDKDPAK AINAAKVVVA FIVAGSPDLV 250 260 270 280 290 300 LERHGIPVDA KKQIGDAIAK GDFGALMGGL VTPQMIEAFA ICGTPEDCMK RIKDLEAIGV 310 320 TQIVAGSPIG PEKEKAIKLI GKEIIAKM

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07085.1.
RefSeq	NP_618605.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TJP7.
SMR	Q8TJP7. Positions 1-327.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA3733.
Proteomic databases
PRIDE	Q8TJP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM07085; AAM07085; MA_3733.
GeneID	1475626.
KEGG	mac:MA3733.
Phylogenomic databases
eggNOG	COG2141.
KO	K00320.
OMA	PALKIAY.
ProtClustDB	PRK02271.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-3798-MONOMER.
UniPathway	UPA00640; UER00697.
Family and domain databases
Gene3D	3.20.20.30. 1 hit.
HAMAP	MF_01091. F420_mer.
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METAC

Accession

Primary (citable) accession number: Q8TJP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program