Reviewed, UniProtKB/Swiss-Prot Q8TJP7 (MER_METAC)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydromethanopterin reductase
    EC=1.5.99.11
Alternative name(s):
    Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
    Methylene-H(4)MPT reductase

Gene names

Name:	mer
Ordered Locus Names:	MA_3733

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity.
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_0000084806

Sequences

	Sequence		Length	Mass (Da)
	Q8TJP7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: C57ABD6B163CBD4E	FASTA	328	34,720
	10 20 30 40 50 60 MKFGIEFVPS DPALKIAYYA KLSEQQGFDY VWITDHYNNR DVYSTLTVLA LNTNSIKIGS 70 80 90 100 110 120 GVTNSYTRNP AITASSIASI AEISGGRAVL GLGPGDKATF DAMGIAWEKP LATTKEAIQA 130 140 150 160 170 180 IRDFIAGKKV SMDGEMVKFA GAKLAFKAGN VPIYMGAQGP KMLELAGEVA DGVLINASHP 190 200 210 220 230 240 KDFEVAVEQI RKGAEKVGRD PSEVDVTAYA CFSIDKDPAK AINAAKVVVA FIVAGSPDLV 250 260 270 280 290 300 LERHGIPVDA KKQIGDAIAK GDFGALMGGL VTPQMIEAFA ICGTPEDCMK RIKDLEAIGV 310 320 TQIVAGSPIG PEKEKAIKLI GKEIIAKM

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM07085.1.
RefSeq	NP_618605.1.
3D structure databases
HSSP	HSSP built from PDB template 1F07 based on UniProtKB Q50744.
SMR	Q8TJP7. Positions 1-327.
ModBase	Search...
Genome annotation databases
GeneID	1475626.
GenomeReviews	Gene locus MA_3733 in contig AE010299_GR.
KEGG	mac:MA3733.
NMPDR	fig|188937.1.peg.3631.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TJP7.
OMA	LNEISDG.
Enzyme and pathway databases
BioCyc	MACE188937:MA3733-MON.
BRENDA	1.5.99.11. 275708.
Family and domain databases
HAMAP	MF_01091. [Tree]
InterPro	IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METAC

Accession

Primary (citable) accession number: Q8TJP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)