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Protein

Arsenite methyltransferase

Gene

arsM

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from AdoMet to arsenite, producing methylated arsenicals. Involved in the conversion of As(III) to a number of methylated products. Reduces the arsenic toxicity in the cell and may contribute to the global arsenic cycling.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + arsenite = S-adenosyl-L-homocysteine + methylarsonate.1 Publication
S-adenosyl-L-methionine + methylarsonite = S-adenosyl-L-homocysteine + dimethylarsinate.1 Publication

Enzyme regulationi

Highly dependent on the characteristics of the thiol cofactors used, with some of them (coenzyme M, homocysteine, and dithiothreitol) more efficient than GSH.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processArsenical resistance
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-4023-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Arsenite methyltransferase1 Publication (EC:2.1.1.1371 Publication)
Alternative name(s):
As(III) methyltransferase1 Publication
Gene namesi
Name:arsM1 Publication
Ordered Locus Names:MA_3783Imported
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi30C → S: No change in activity. 1 Publication1
Mutagenesisi31C → S: No change in activity. 1 Publication1
Mutagenesisi62C → S: Strong decrease in activity. Can still methylate monomethylarsenic. 1 Publication1
Mutagenesisi150C → S: Lack of activity. 1 Publication1
Mutagenesisi194C → S: No change in activity. 1 Publication1
Mutagenesisi200C → S: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004395961 – 249Arsenite methyltransferaseAdd BLAST249

Interactioni

Protein-protein interaction databases

STRINGi188937.MA3783

Structurei

3D structure databases

ProteinModelPortaliQ8TJK1
SMRiQ8TJK1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01792 Archaea
ENOG410XSKB LUCA
InParanoidiQ8TJK1
KOiK07755
OMAiYAGCVSG
OrthoDBiPOG093Z0A8C
PhylomeDBiQ8TJK1

Family and domain databases

InterProiView protein in InterPro
IPR025714 Methyltranfer_dom
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF13847 Methyltransf_31, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TJK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAAEKKEVI KKKYQEIATL GGSCCSGGGC CGDLSAADLS RSLGYSEADV
60 70 80 90 100
QAVPDANLGL GCGNPTAFAE LKPGDIVLDL GSGAGFDSFL AAQRVGSLGK
110 120 130 140 150
VIGVDMTQEM VKKAQDNARK YGYSNVEFRQ GDIEALPLDD RSVDVIISNC
160 170 180 190 200
VINLAPDKEK VFREAFRVLK PGGRMYVSDM VLLEDLPEDL KNDCDLLAGC
210 220 230 240
VAGALLKEEY LGLLKKAGFS FKILAEDSDV SKRQYEGLPV ESLKLKAWV
Length:249
Mass (Da):26,671
Last modified:June 1, 2002 - v1
Checksum:i032FAF1A05B085BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM07134.1
RefSeqiWP_011023683.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM07134; AAM07134; MA_3783
GeneIDi1475676
KEGGimac:MA_3783

Similar proteinsi

Entry informationi

Entry nameiARSM_METAC
AccessioniPrimary (citable) accession number: Q8TJK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health