Putative K(+)-stimulated pyrophosphate-energized sodium pump - Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Putative K(+)-stimulated pyrophosphate-energized sodium pump
EC=3.6.1.1

Alternative name(s):
Membrane-bound sodium-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name=Na(+)-PPase

Gene names

Name:	hppA1
Ordered Locus Names:	MA_3879

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na⁺ movement across the membrane By similarity. HAMAP MF_01129
Catalytic activity	Diphosphate + H₂O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium By similarity. HAMAP MF_01129
Enzyme regulation	Requires K⁺ for maximal activity By similarity. HAMAP MF_01129
Subunit structure	Homodimer By similarity. HAMAP MF_01129
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]
Sequence caution	The sequence AAM07230.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ion transport Sodium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Potassium Sodium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 676

676

Putative K(+)-stimulated pyrophosphate-energized sodium pump HAMAP MF_01129

PRO_0000217006

Regions

Transmembrane

3 – 23

21

Helical; Potential

Transmembrane

58 – 78

21

Helical; Potential

Transmembrane

83 – 103

21

Helical; Potential

Transmembrane

129 – 149

21

Helical; Potential

Transmembrane

158 – 178

21

Helical; Potential

Transmembrane

241 – 261

21

Helical; Potential

Transmembrane

265 – 285

21

Helical; Potential

Transmembrane

303 – 323

21

Helical; Potential

Transmembrane

327 – 347

21

Helical; Potential

Transmembrane

390 – 410

21

Helical; Potential

Transmembrane

411 – 431

21

Helical; Potential

Transmembrane

469 – 489

21

Helical; Potential

Transmembrane

501 – 521

21

Helical; Potential

Transmembrane

572 – 592

21

Helical; Potential

Transmembrane

593 – 613

21

Helical; Potential

Transmembrane

656 – 676

21

Helical; Potential

Sites

Site

463

1

Determinant of potassium dependence By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TJA9 [UniParc].

Last modified July 25, 2003. Version 2.
Checksum: 5C7144B63F1F8E26
Show »

FASTA

676

69,277

        10         20         30         40         50         60 
MDMLIYLAPI CALIGLIFAG ISYKNVQNEG AGNDLIKKIT ASIHGGAMVY LNRQYRAIAV 

        70         80         90        100        110        120 
FVVFLAIIIA LILPNGALTA ACFVFGAVLS ATAGYAGMLT ATIANGRTTN AATRGIGPAF 

       130        140        150        160        170        180 
RVSFASGTVM GMSVVGLGLF GLSLSFIILE SVYTDLDLLT IVNIVAGFSL GASSIALFAR 

       190        200        210        220        230        240 
VGGGIFTKAA DVGADLVGKV EAGIPEDDPR NPAVIADNVG DNVGDIAGMG ADLYESYVGS 

       250        260        270        280        290        300 
ILATMLLAAS TAATTFPNIP VENVILVPLI ISAIGILASI VGTFFVRTNK TESSAIHMAF 

       310        320        330        340        350        360 
NMGLIAAIIL TVIASYFVTS MLLGEYGLNV FFATVAGLVA GFLIGQITEH YTSYDRKPTL 

       370        380        390        400        410        420 
RVANSCQTGS ATNIITGFAK GMESTLWPVV IISIAIYIAF QLSGLYGIAI AAVGMLATLG 

       430        440        450        460        470        480 
ISLSVDAYGP VADNAGGIAE MSHQKEEVRQ ITDTLDAVGN TTAAIGKGFA IGSAALTALA 

       490        500        510        520        530        540 
LFASYGIAVG LSAIDVMNPN VFIGLTIGAM LPYLFSSMTI LAVGNAAGEV VVEVRRQFRE 

       550        560        570        580        590        600 
IAGLMEGKAD PDYGKCIAIS THSALKEMIP PGLLAVIAPL LVGLVLGPGA LGGLLAGSVA 

       610        620        630        640        650        660 
SGFMIAITMS NAGGAWDNAK KYIELGNFGG KGSDAHKAGV TGDTVGDPFK DTAGPAINIL 

       670 
IKLMSIVAVV FAPLFM

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References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.

Birren B.
Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07230.1. Different initiation.
RefSeq	NP_618750.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475772.
GenomeReviews	Gene locus MA_3879 in contig AE010299_GR.
KEGG	mac:MA3879.
NMPDR	fig\|188937.1.peg.3776.
Phylogenomic databases
HOGENOM	HBG593668.
OMA	NKTESSA.
ProtClustDB	PRK00733.
Enzyme and pathway databases
BioCyc	MACE188937:MA3879-MONOMER.
Family and domain databases
HAMAP	MF_01129. PPase-energized_pump. [Tree]
InterPro	IPR004131. PPase-energised_H-pump. [Graphical view]
KO	K01507.
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA1_METAC

Accession

Primary (citable) accession number: Q8TJA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	July 25, 2003
Last modified:	November 16, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents