Reviewed, UniProtKB/Swiss-Prot Q8TJA8 (HPPA2_METAC)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump 2
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase 2
      Short name=H(+)-PPase 2
    Membrane-bound proton-translocating pyrophosphatase 2

Gene names

Name:	hppA2
Ordered Locus Names:	MA_3880

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	Pyrophosphate-energized proton pump 2 HAMAP MF_01130		PRO_0000217036
Regions
	Transmembrane	5 – 27	23	Potential
	Transmembrane	57 – 74	18	Potential
	Transmembrane	79 – 101	23	Potential
	Transmembrane	122 – 144	23	Potential
	Transmembrane	154 – 176	23	Potential
	Transmembrane	227 – 244	18	Potential
	Transmembrane	248 – 270	23	Potential
	Transmembrane	282 – 301	20	Potential
	Transmembrane	311 – 333	23	Potential
	Transmembrane	354 – 376	23	Potential
	Transmembrane	391 – 413	23	Potential
	Transmembrane	490 – 512	23	Potential
	Transmembrane	553 – 575	23	Potential
	Transmembrane	580 – 599	20	Potential
	Transmembrane	644 – 666	23	Potential
Sites
	Site	451	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TJA8-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 45E43BD57F4B30F7	FASTA	671	69,017
	10 20 30 40 50 60 MESLIFIAPL AGVISLVFAA FFAKSILKED AGNKRMKEIA GAIQEGAMAY LNRQYKTIAV 70 80 90 100 110 120 VSIILSFLIL FLLDDGLKIA IGFLAGAISS AAAGYIGMSV SVRANIRTAH AASSGLEKAM 130 140 150 160 170 180 SVAFRGGAVT GLAVVGLALL GTSSFYILYG DVDLVVGFGF GASLISLFAR VGGGIFTKAA 190 200 210 220 230 240 DVGADLVGKV EAGIPEDDPR NAGVIADNVG DNVGDCAGMG ADLFETYVVT SLAAMLLGSL 250 260 270 280 290 300 IIGTYENAIL YPLVLGSVAI FASIISVFFV KIGKEGKIMQ ALYKGVGGSA IISLIAFYFV 310 320 330 340 350 360 TNSLMGDIRL FYATVVGIII TVLMVIITEY YTSTDYRPVK TIAASSETGA ATNIISGLSI 370 380 390 400 410 420 GFESTLVPTV VIVIGILISY FIVGGAADAG IGLYGIAIAA VAMLSTTGMI VALDSYGPIT 430 440 450 460 470 480 DNAGGIAQMA NLPAQVRKVT DALDAVGNTT KAVTKGYAIG SAALGALALF ADYRSKVNLG 490 500 510 520 530 540 GQSLNLDDPV VLAGLLLGAL LPFVFSAVTM SAVGKAAFEV VNEVRRQFRE IPGIMEGTAK 550 560 570 580 590 600 PEYGRCVDIV TKAALHEMAM PGFLAVLVPL LVGLILGPKA LAGLLIGLIV VGFMLALMMD 610 620 630 640 650 660 NGGGAWDNAK KLIEDGNHGG RGSEAHKAAV VGDTVGDPFK DTAGPALNAL IKVVNMVAIL 670 FSSLIIHSGL F

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM07231.1.
RefSeq	NP_618751.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1475773.
GenomeReviews	Gene locus MA_3880 in contig AE010299_GR.
KEGG	mac:MA3880.
NMPDR	fig|188937.1.peg.3777.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TJA8.
OMA	KMATRAN.
Enzyme and pathway databases
BioCyc	MACE188937:MA3880-MON.
BRENDA	3.6.1.1. 275708.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA2_METAC

Accession

Primary (citable) accession number: Q8TJA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)