Q8TIT6 (PURL_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	MA_4055

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100512
Regions
	Nucleotide binding	90 – 101	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TIT6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A3C22E07EC6F1D43	FASTA	715	76,231
	10 20 30 40 50 60 MLPEEDLKII RKELGREPTL VEQGCFLNLW SEHCSYRSSA PLLKTFTSTG ENVLIGPGDD 70 80 90 100 110 120 AAIIKFEDGY VLAIGMESHN HPSYVDPYNG AATGIGGIVR DIISMGARPI ALMDPLYFGP 130 140 150 160 170 180 LDTPKNLFLF EQIIKGIAGY GNCIGVPVVN GETFFDRRYS GNPLVNVVAV GLCKEENVTT 190 200 210 220 230 240 SRSQKAENKL ILAGSSTGKD GLGGASFASR DLSESAEAED RPSVQVGDPY TEKLVMEMTL 250 260 270 280 290 300 EAIEKGYVKS CKDLGAAGLG GASSELAAKG GLGARIIADA VPQREPNMNA YEILLAESQE 310 320 330 340 350 360 RMLFEVAPED VDAVLALVQK YDLNGAVVGY LTKEPTYTVE FGGEIVADIP IAFLTGGAPT 370 380 390 400 410 420 CEKPSEAPTL REEGKKPETP EDLKVAFLKV LSSYNIASKE WIYRQYDHEV QLRTVVKPGE 430 440 450 460 470 480 DSGVLRITGT KGIALTCGCQ PRATLLDPYN GGKTAIIENS MNLAVKGAEP LAIVNCLNFG 490 500 510 520 530 540 NPERPETYWQ FENAVLGLGD EARKLSIPVV GGNVSLYNES DEFKTAIPPT PSIGMIGKVD 550 560 570 580 590 600 LEIPLPSGFF AKDGDSIILV GETTPEMGGS EYYACMDSGN AGMVPAVPEN APELIKAIIE 610 620 630 640 650 660 AVKSGKLSSA HDLSLGGIGA GLARMCRSMG AKVDLSELAG ETQADELLFS EAPARALLAI 670 680 690 700 710 SEPEAVREIL KDVPHAVIGK VGGETLEIKG KNFELSVSLK EIAEAYGSLT RFMMG

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07403.1.
RefSeq	NP_618923.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TIT6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1475949.
GenomeReviews	Gene locus MA_4055 in contig AE010299_GR.
KEGG	mac:MA4055.
NMPDR	fig|188937.1.peg.3949.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	MACE188937:MA4055-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METAC

Accession

Primary (citable) accession number: Q8TIT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program