Q8TIB8 (HDRB_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CoB--CoM heterodisulfide reductase 1 subunit B
EC=1.8.98.1

Gene names

Name:	hdrB
Ordered Locus Names:	MA_4237

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrB may have a function in energy transduction By similarity.
Catalytic activity	Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine.
Pathway	Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.
Subunit structure	The heterodisulfide reductase 1 is composed of three subunits; hdrA, hdrB and hdrC By similarity.
Sequence similarities	Belongs to the hdrB family.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cofactor metabolic process Inferred from electronic annotation. Source: InterPro methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	CoB--CoM heterodisulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	CoB--CoM heterodisulfide reductase 1 subunit B		PRO_0000150064

Sequences

	Sequence		Length	Mass (Da)
	Q8TIB8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: F297B43D298990DC	FASTA	300	32,819
	10 20 30 40 50 60 MAKLSLFRGC IVPNRYPGIE KATKLCLQKL EVDAVDLPGA SCCPAPGVFK SFDKATWLAL 70 80 90 100 110 120 ASRNIVLSER MGRDILTVCN GCYGSLADAN IELKNDPEMK ACTNSCLKEI GMEYKGTAEV 130 140 150 160 170 180 RHIIEFLYKE LGPEKLKSFI TTPLDLKVAL HYGCHLIKPS KERNLGETEA PVFFDELVEA 190 200 210 220 230 240 TGAKSVDYTD KMMCCGAGGG VRSGHAAESL EMLEHKLACI RNAGVDCIVN ACPFCHLQFD 250 260 270 280 290 300 RGQLAVNEKF GTDYSIPVLH YSQLLGLALG FSPDELGIEQ NAIQNIEFLA KIYEISAGLR

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07582.1.
RefSeq	NP_619102.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476131.
GenomeReviews	Gene locus MA_4237 in contig AE010299_GR.
KEGG	mac:MA4237.
NMPDR	fig|188937.1.peg.4128.
Phylogenomic databases
HOGENOM	HBG513215.
OMA	HYGCHFL.
ProtClustDB	CLSK876313.
Enzyme and pathway databases
BioCyc	MACE188937:MA4237-MONOMER.
Family and domain databases
InterPro	IPR017678. CoB/CoM_hetero-S_Rdtase_bsu. IPR004017. Cys_rich_dom. [Graphical view]
KO	K03389.
Pfam	PF02754. CCG. 2 hits. [Graphical view]
TIGRFAMs	TIGR03288. CoB_CoM_SS_B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDRB_METAC

Accession

Primary (citable) accession number: Q8TIB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program