Reviewed, UniProtKB/Swiss-Prot Q8TI34 (OGT_METAC)

Names and origin

Protein names

Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    EC=2.1.1.63
Alternative name(s):
    6-O-methylguanine-DNA methyltransferase
      Short name=MGMT
    O-6-methylguanine-DNA-alkyltransferase

Gene names

Name:	ogt
Ordered Locus Names:	MA_4322

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity.
Catalytic activity	DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP MF_00772
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MGMT family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA dealkylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Methylated-DNA--protein-cysteine methyltransferase HAMAP MF_00772		PRO_0000139376
Sites
	Active site	126	1	Nucleophile; methyl group acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TI34-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 0B54966FE1B59981	FASTA	156	17,337
	10 20 30 40 50 60 MYYAIFESPI GPILLAGDEE GLKYVNFMKG KKKIEVPDSW VENEEFFREA SRQLEAYFAG 70 80 90 100 110 120 ELKSFDVKLA PEGTEFQKSV WNALKEIPYG ETRTYGEIAK NIGNPKASRA VGLANNRNPI 130 140 150 AIIVPCHRVI GANGKLTGYA SGLDIKEFLL KLEGSC

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010299 Genomic DNA. Translation: AAM07666.1.
RefSeq	NP_619186.1.
3D structure databases
HSSP	HSSP built from PDB template 1SFE based on UniProtKB P06134.
ModBase	Search...
Genome annotation databases
GeneID	1476216.
GenomeReviews	Gene locus MA_4322 in contig AE010299_GR.
KEGG	mac:MA4322.
NMPDR	fig|188937.1.peg.4212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8TI34.
OMA	Q8TI34. TRSYGEL.
Enzyme and pathway databases
BioCyc	MACE188937:MA4322-MON.
BRENDA	2.1.1.63. 275708.
Family and domain databases
HAMAP	MF_00772. [Tree]
InterPro	IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA_bd. IPR008332. MethylG_MeTrfase. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit.
Pfam	PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00589. ogt. 1 hit.
PROSITE	PS00374. MGMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OGT_METAC

Accession

Primary (citable) accession number: Q8TI34

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)