Q8TI34 (OGT_METAC) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 60.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Methylated-DNA--protein-cysteine methyltransferase EC=2.1.1.63 Alternative name(s): 6-O-methylguanine-DNA methyltransferase Short name=MGMT O-6-methylguanine-DNA-alkyltransferase | ||||
| Gene names |
| ||||
| Organism | Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) | ||||
| Taxonomic identifier | 188937 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina |
Protein attributes
| Sequence length | 156 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity. HAMAP MF_00772 |
| Catalytic activity | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP MF_00772 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00772. |
| Miscellaneous | This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles. HAMAP MF_00772 |
| Sequence similarities | Belongs to the MGMT family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair |
| Cellular component | Cytoplasm |
| Molecular function | Methyltransferase Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | DNA repair Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 156 | 156 | Methylated-DNA--protein-cysteine methyltransferase HAMAP MF_00772 | PRO_0000139376 | |||||
Sites | |||||||||
| Active site | 126 | 1 | Nucleophile; methyl group acceptor By similarity | ||||||
Sequences
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References
| [1] | "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. Birren B.Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE010299 Genomic DNA. Translation: AAM07666.1. |
| RefSeq | NP_619186.1. NC_003552.1. |
3D structure databases | |
| ProteinModelPortal | Q8TI34. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1476216. |
| GenomeReviews | Gene locus MA_4322 in contig AE010299_GR. |
| KEGG | mac:MA4322. |
| NMPDR | fig|188937.1.peg.4212. |
Phylogenomic databases | |
| HOGENOM | HBG752398. |
| OMA | MANSRNP. |
| ProtClustDB | PRK00901. |
Enzyme and pathway databases | |
| BioCyc | MACE188937:MA4322-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00772. OGT. [Tree] |
| InterPro | IPR023546. MethylDNA_cys_MeTrfase. IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA-bd. IPR008332. MethylG_MeTrfase. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| KO | K00567. |
| Pfam | PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view] |
| SUPFAM | SSF46767. MethylDNA_cys_mtrans_DNA_bd. 1 hit. |
| TIGRFAMs | TIGR00589. Ogt. 1 hit. |
| PROSITE | PS00374. MGMT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | OGT_METAC | ||||||||
| Accession | Primary (citable) accession number: Q8TI34 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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