Reviewed,
UniProtKB/Swiss-Prot Q8TI34 (OGT_METAC)
Last modified
June 16, 2009.
Version 42.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Methylated-DNA--protein-cysteine methyltransferase EC=2.1.1.63 Alternative name(s): 6-O-methylguanine-DNA methyltransferase Short name=MGMT O-6-methylguanine-DNA-alkyltransferase | ||||
| Gene names |
| ||||
| Organism | Methanosarcina acetivorans [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 2214 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina |
Protein attributes
| Sequence length | 156 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated By similarity. |
| Catalytic activity | DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. HAMAP MF_00772 |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the MGMT family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA damage DNA repair |
| Cellular component | Cytoplasm |
| Molecular function | Methyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA dealkylation Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | methylated-DNA-[protein]-cysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 156 | 156 | Methylated-DNA--protein-cysteine methyltransferase HAMAP MF_00772 | PRO_0000139376 | |||||
Sites | |||||||||
| Active site | 126 | 1 | Nucleophile; methyl group acceptor By similarity | ||||||
Sequences
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References
| [1] | "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. Birren B.Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A. |
Cross-references
Sequence databases | |
|---|---|
| AE010299 Genomic DNA. Translation: AAM07666.1. | |
| RefSeq | NP_619186.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1SFE based on UniProtKB P06134. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1476216. |
| GenomeReviews | Gene locus MA_4322 in contig AE010299_GR. |
| KEGG | mac:MA4322. |
| NMPDR | fig|188937.1.peg.4212. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q8TI34. |
| OMA | Q8TI34. TRSYGEL. |
Enzyme and pathway databases | |
| BioCyc | MACE188937:MA4322-MON. |
| BRENDA | 2.1.1.63. 275708. |
Family and domain databases | |
| HAMAP | MF_00772. [Tree] |
| InterPro | IPR001497. MethylDNA_cys_MeTrfase_AS. IPR014048. MethylDNA_cys_MeTrfase_DNA_bd. IPR008332. MethylG_MeTrfase. IPR011991. Wing_hlx_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit. |
| PANTHER | PTHR10815. MethylDNA_cys_mtrans_DNA_bd. 1 hit. |
| Pfam | PF01035. DNA_binding_1. 1 hit. PF02870. Methyltransf_1N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00589. ogt. 1 hit. |
| PROSITE | PS00374. MGMT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | OGT_METAC | ||||||||
| Accession | Primary (citable) accession number: Q8TI34 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

Clusters with


