ID   MTD_METAC               Reviewed;         279 AA.
AC   Q8THT2;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   16-JUN-2009, entry version 42.
DE   RecName: Full=F420-dependent methylenetetrahydromethanopterin dehydrogenase;
DE            Short=MTD;
DE            EC=1.5.99.9;
DE   AltName: Full=Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase;
GN   Name=mtd; OrderedLocusNames=MA_4430;
OS   Methanosarcina acetivorans.
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2214;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   MEDLINE=21929760; PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P.,
RA   FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A.,
RA   Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R.,
RA   Linton L., McEwan P., McKernan K., Talamas J., Tirrell A., Ye W.,
RA   Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M.,
RA   Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A.,
RA   Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K.,
RA   Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C.,
RA   Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T.,
RA   Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E.,
RA   Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic
RT   and physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- FUNCTION: Catalyzes the reversible reduction of methenyl-
CC       H(4)MPT(+) to methylene-H(4)MPT (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydromethanopterin +
CC       coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced
CC       coenzyme F420.
CC   -!- PATHWAY: One-carbon metabolism; methanogenesis from carbone
CC       dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT
CC       (cofactor F420 route): step 1/1.
CC   -!- SIMILARITY: Belongs to the MTD family.
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DR   EMBL; AE010299; AAM07771.1; -; Genomic_DNA.
DR   RefSeq; NP_619291.1; -.
DR   GeneID; 1476324; -.
DR   GenomeReviews; AE010299_GR; MA_4430.
DR   KEGG; mac:MA4430; -.
DR   NMPDR; fig|188937.1.peg.4317; -.
DR   HOGENOM; Q8THT2; -.
DR   OMA; Q8THT2; NPYAKAK.
DR   BioCyc; MACE188937:MA4430-MON; -.
DR   BRENDA; 1.5.99.9; 275708.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0030268; F:methylenetetrahydromethanopterin dehydrogen...; IEA:HAMAP.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00058; -; 1.
DR   InterPro; IPR002844; Methylene_DH.
DR   Pfam; PF01993; MTD; 1.
DR   PIRSF; PIRSF005627; MTD; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methanogenesis; One-carbon metabolism;
KW   Oxidoreductase.
FT   CHAIN         1    279       F420-dependent
FT                                methylenetetrahydromethanopterin
FT                                dehydrogenase.
FT                                /FTId=PRO_0000075037.
SQ   SEQUENCE   279 AA;  30268 MW;  2AB2991081836567 CRC64;
     MRKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMGPADA ADTESFKQWN
     ADFVVMISPN AAAPGPTAAR EIWKDVPCIV VSDGPTKKEA REALEQEGFG YIILPVDPLI
     GAKREFLDPV EMASFNSDAM KVLSSCGVVR LIQEELDRVT EQVASGKSGE DLELPHIFAK
     PEKCVEHAGF ANPYAKAKAL AALHMAEKVA QVNFPACFML KEVEQVCLTA AAGHEIMGAA
     AILATQAREI EKSNDTVFRQ PHAKNGTLLK KVKLYEKPE
//