Reviewed, UniProtKB/Swiss-Prot Q8THT2 (MTD_METAC)

Names and origin

Protein names

Recommended name:
    F420-dependent methylenetetrahydromethanopterin dehydrogenase
      Short name=MTD
    EC=1.5.99.9
Alternative name(s):
    Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	MA_4430

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity. HAMAP MF_00058
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_0000075037

Sequences

	Sequence		Length	Mass (Da)
	Q8THT2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2AB2991081836567	FASTA	279	30,268
	10 20 30 40 50 60 MRKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMGPADA ADTESFKQWN 70 80 90 100 110 120 ADFVVMISPN AAAPGPTAAR EIWKDVPCIV VSDGPTKKEA REALEQEGFG YIILPVDPLI 130 140 150 160 170 180 GAKREFLDPV EMASFNSDAM KVLSSCGVVR LIQEELDRVT EQVASGKSGE DLELPHIFAK 190 200 210 220 230 240 PEKCVEHAGF ANPYAKAKAL AALHMAEKVA QVNFPACFML KEVEQVCLTA AAGHEIMGAA 250 260 270 AILATQAREI EKSNDTVFRQ PHAKNGTLLK KVKLYEKPE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07771.1.
RefSeq	NP_619291.1.
3D structure databases
SMR	Q8THT2. Positions 4-279.
ModBase	Search...
Genome annotation databases
GeneID	1476324.
GenomeReviews	Gene locus MA_4430 in contig AE010299_GR.
KEGG	mac:MA4430.
NMPDR	fig|188937.1.peg.4317.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG538567.
OMA	IVASAHE.
Enzyme and pathway databases
BioCyc	MACE188937:MA4430-MONOMER.
BRENDA	1.5.99.9. 275708.
Family and domain databases
HAMAP	MF_00058. MTD. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProDom	PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MTD_METAC

Accession

Primary (citable) accession number: Q8THT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2002
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)