Reviewed, UniProtKB/Swiss-Prot Q8THK3 (GUAAB_METAC)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Synonyms:	guaA
Ordered Locus Names:	MA_4511

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_00345
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140240
Regions
	Domain	33 – 185	153	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8THK3-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 5D97F73EB1FC8251	FASTA	305	34,196
	10 20 30 40 50 60 MVKPEKFIPK AIEKISQEIK EGKAIIALSG GVDSSVCAEL AYRAIGDRLQ PIYIDTGLMR 70 80 90 100 110 120 KGETERIKHI FSHMNLHVVY AKDRFLEALA GVTDPEEKRK AVGETFIRVF EDEAKRIAAD 130 140 150 160 170 180 YLIQGTIYPD RIESEGGIKS HHNVGGLPSV MDFEKIVEPI EDLYKDEVRE VAWALQLPEE 190 200 210 220 230 240 ICERMPFPGP GLAVRILGEV TEEKLEVVRE ANFIVEEELL ERFCPWQTFA AVLGKGTGVK 250 260 270 280 290 300 GDIRAYGWIV AVRAVGSRDG MTAEALELPW EVLKHLEARI TSEIPKVARV VYDITPKPPA TIEFE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07851.1.
RefSeq	NP_619371.1.
3D structure databases
SMR	Q8THK3. Positions 4-305.
ModBase	Search...
Genome annotation databases
GeneID	1476405.
GenomeReviews	Gene locus MA_4511 in contig AE010299_GR.
KEGG	mac:MA4511.
NMPDR	fig|188937.1.peg.4397.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	GIKSHHN.
Enzyme and pathway databases
BioCyc	MACE188937:MA4511-MONOMER.
BRENDA	6.3.5.2. 275708.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_METAC

Accession

Primary (citable) accession number: Q8THK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)