Q8THH3 (AROA_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MA_4544

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Probable 3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088328

Sequences

	Sequence		Length	Mass (Da)
	Q8THH3 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7C4BB54968203405	FASTA	430	45,846
	10 20 30 40 50 60 MRVSISKSSV KGEVFAPSSK SYTHRAITLA ALSNESIVRR PLLSADTLAT IRASEMFGAS 70 80 90 100 110 120 VKREEENLII HGFNGKPNVP DDVIDAANSG TTLRLMTAIA GLTDGITVLT GDSSLRTRPN 130 140 150 160 170 180 GPLLKTLNQL GASACSTRGN EKAPLVVKGG LEGKKVSIEG SISSQFISAL LIACPLAENS 190 200 210 220 230 240 TTLSIIGKLK SRPYVDVTIE MLELAGVKIH TDENNGTKFI IPGKQKYDLK EYTIPGDFSS 250 260 270 280 290 300 ASYLLAAAAM TEGSEITVKN LFPSKQGDKL IIETLKQMGA DITWDREAGI VTVRGGRKLK 310 320 330 340 350 360 AVTFDAGATP DLVPTVAVLA AVAEGTSRIE NAEHVRYKET DRLSALATEL PKLGVKLKEE 370 380 390 400 410 420 KDSLTITGGE LKGAEVHGWD DHRIVMSLAL AGMVAGNTTI DTTESVAISY PDFFEDMSNL 430 GVKIKQISEE

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07883.1.
RefSeq	NP_619403.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8THH3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476438.
GenomeReviews	Gene locus MA_4544 in contig AE010299_GR.
KEGG	mac:MA4544.
NMPDR	fig|188937.1.peg.4429.
Phylogenomic databases
HOGENOM	HBG646626.
OMA	RLRIKEC.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	MACE188937:MA4544-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_METAC

Accession

Primary (citable) accession number: Q8THH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program