Q8THG5 (DP2L_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MA_4552

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	1145 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1145	1145	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152574

Sequences

	Sequence		Length	Mass (Da)
	Q8THG5 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 36C329A400C09E43	FASTA	1,145	127,679
	10 20 30 40 50 60 MGETIASEEM HEYFDGLEAR LKEAIEIANR ARAQGGDPKP TVEIPLAKDL ADRVENLIGV 70 80 90 100 110 120 QGVAAKIREL ETRMSREEAA LEIGKQVAEG VVGSFPTKKD AVEAAIRVSM ATLTEGVVAA 130 140 150 160 170 180 PIEGIDKVEL GKNDDGSQYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPREE 190 200 210 220 230 240 EVERYVEEIL LYKRVASLQY TPSEDEIRLI VRNCPVCIDG DPTEEAEVEG HRDLERIGTN 250 260 270 280 290 300 RVRGGMCLVL AEGLALKAPK VKKHVNKLKM DGWDWLETLI GGAKSGGSAE DEQKSKIKPK 310 320 330 340 350 360 DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFASAGI NPASMVLLDD FITNGTQLKV 370 380 390 400 410 420 ERPGKAAAMS AVDSIEGPTV RLFSGDLVRI DNIKEAYELR PQVESIVDIG EILINYGDFL 430 440 450 460 470 480 ENNHPLMPSP YVFEWWQYDY EAACPEKTPE EELKNPSSAL ALRLAEEYNV PLHPTFTYLW 490 500 510 520 530 540 HDINRNEFEA LRKFVVEKGT FDGEGTLKLP LTASFEEGIK PLLEKLLVLH RVKEDKILVE 550 560 570 580 590 600 EALPFVLCLG LDSSLKEKAG MPDTDDMVEA AGVLSGFKVY PRAPSRIGAR MGRPEKSDLR 610 620 630 640 650 660 KMSPAAQVLF PINNCGGITR NLVSASDYTS CMNGKIGEIE VELGLRECPA CGKETYFWRC 670 680 690 700 710 720 ECGEFTNPKL SCPRCKIDVR GAETCPKCGR KPTSVANVKL DFRSVYKQAF ENVGEREKMD 730 740 750 760 770 780 LIKGVKRLMN GQMTPEPLEK GILRAKHDVY IFKDGTVRYD MSDIPLTHIR ADELGITATK 790 800 810 820 830 840 LLELGYREDI YGNPLERDDQ VVCLKVQDLV ISYDGGEYML RTAKYVDDLL VKYYKVGPYY 850 860 870 880 890 900 NAETIQDLVG VLLIGLAPHT SAGVLGRLIG FTKASVGYAH PFFHASKRRN CDGDEDCIML 910 920 930 940 950 960 LMDGILNFSR SYLPDKRGGK MDAPLVLTTR IDPKEVDKEA HNIDVPARYP LEFYRATQEI 970 980 990 1000 1010 1020 KNPTELESIM DLISGRLGTP EQYEHFMFTH DTSDIAAGPL NSSYKTLGSM IEKMEAQLSL 1030 1040 1050 1060 1070 1080 ANRIRAVDAP DVAERVLKSH FLPDLIGNLR SFSRQRMRCI KCGEKFRRPP LTGACPKCGG 1090 1100 1110 1120 1130 1140 NVVLTVHEGA VRKYLEISKE IGERYGVSSY TRQRIELLDY DICSLFENHK VKQLGLSDFM SGSAR

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM07891.1.
RefSeq	NP_619411.1. NC_003552.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1476446.
GenomeReviews	Gene locus MA_4552 in contig AE010299_GR.
KEGG	mac:MA4552.
NMPDR	fig|188937.1.peg.4437.
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	MACE188937:MA4552-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METAC

Accession

Primary (citable) accession number: Q8THG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	June 1, 2002
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program