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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. no protein annotated in this organism
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ShikimateUniRule annotation1
Active sitei66Proton acceptorUniRule annotation1
Binding sitei88ShikimateUniRule annotation1
Binding sitei104ShikimateUniRule annotation1
Binding sitei222NADP; via carbonyl oxygenUniRule annotation1
Binding sitei224ShikimateUniRule annotation1
Binding sitei245NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132NADPUniRule annotation5
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-4903-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:MA_4594
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360591 – 280Shikimate dehydrogenase (NADP(+))Add BLAST280

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA4594

Structurei

3D structure databases

ProteinModelPortaliQ8THC3
SMRiQ8THC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01033 Archaea
COG0169 LUCA
InParanoidiQ8THC3
KOiK00014
OMAiFGNPIKH
OrthoDBiPOG093Z09ZR
PhylomeDBiQ8THC3

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8THC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVFGVFGD PVGHSLSPAM HNSAFSALGM DCIYHAFRVR PENLRKAILG
60 70 80 90 100
AEAMGFGGLN LTVPLKEEAL KLDFIRPDPL AERIGAVNTV VFSETGEIRG
110 120 130 140 150
YNTDGLGARQ ALLEAAVEIR GSKVVVAGAG GAARAVAFQL AADGAEITVV
160 170 180 190 200
NRTEERAVEL AKDVAAASLP GKINGTGLSG LKELLRDADI LINTTTLGMH
210 220 230 240 250
PNTDTTIATA EELHSGLTVF DIVYNPLETR LLKEAKVAGA KTVSGVLMLV
260 270 280
YQGAEAFKLW TGVEAPAELM KKTVLEALQA
Length:280
Mass (Da):29,551
Last modified:June 1, 2002 - v1
Checksum:i6D4517D73B9905C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM07933.1
RefSeqiWP_011024467.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM07933; AAM07933; MA_4594
GeneIDi1476488
KEGGimac:MA_4594

Similar proteinsi

Entry informationi

Entry nameiAROE_METAC
AccessioniPrimary (citable) accession number: Q8THC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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