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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATPUniRule annotation
Active sitei325 – 3251Tele-AMP-histidine intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi300 – 3045ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:GI2O-4699-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:MA_4637
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355RNA 3'-terminal phosphate cyclasePRO_0000156425Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi188937.MA4637.

Structurei

3D structure databases

ProteinModelPortaliQ8TH85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0430.
InParanoidiQ8TH85.
KOiK01974.
OMAiMGDQIIP.
PhylomeDBiQ8TH85.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 3 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TH85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIDGSYGE GGGQVVRTAV ALSAVTGKDV KVTNIRKNRP NPGLKQQHLK
60 70 80 90 100
ALETAARICE ARVSGLFPGS SELFFSPVEI KGGKYEVDIG TAGSITLLLQ
110 120 130 140 150
SLMPALPFAK EKVKLTIKGG TDVAWSPTID YLQHVTLRAL EQLGYAGIVT
160 170 180 190 200
LKKRGYYPRG GGEVSAVFEP CKLRSFHFRR VKENRRTGEA RREEETRNEG
210 220 230 240 250
RNQNQRIEIQ GISHASNLPA HVPARQAEAA ESLLLEAGYG SRTKIQSFEL
260 270 280 290 300
LSTGSGITLW SGYCGGSALG KKGLPAEKVG RRAAEEIILE LSSKAPVDVH
310 320 330 340 350
LADQLIPYMA LAGNSSYTVR ELTLHAATNI WVTEQFLDVK FRIEEKEGLF

EVSVD
Length:355
Mass (Da):38,735
Last modified:June 1, 2002 - v1
Checksum:i6E83259BA941176B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM07971.1.
RefSeqiNP_619491.1. NC_003552.1.
WP_011024505.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM07971; AAM07971; MA_4637.
GeneIDi1476531.
KEGGimac:MA4637.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA. Translation: AAM07971.1.
RefSeqiNP_619491.1. NC_003552.1.
WP_011024505.1. NC_003552.1.

3D structure databases

ProteinModelPortaliQ8TH85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi188937.MA4637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM07971; AAM07971; MA_4637.
GeneIDi1476531.
KEGGimac:MA4637.

Phylogenomic databases

eggNOGiCOG0430.
InParanoidiQ8TH85.
KOiK01974.
OMAiMGDQIIP.
PhylomeDBiQ8TH85.

Enzyme and pathway databases

BioCyciMACE188937:GI2O-4699-MONOMER.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 3 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiRTCA_METAC
AccessioniPrimary (citable) accession number: Q8TH85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: April 29, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.