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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPUniRule annotation1
Active sitei325Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi300 – 304ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-4947-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:MA_4637
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001564251 – 355RNA 3'-terminal phosphate cyclaseAdd BLAST355

Interactioni

Protein-protein interaction databases

STRINGi188937.MA4637

Structurei

3D structure databases

ProteinModelPortaliQ8TH85
SMRiQ8TH85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04125 Archaea
COG0430 LUCA
InParanoidiQ8TH85
KOiK01974
OMAiANRPKPG
OrthoDBiPOG093Z05RD
PhylomeDBiQ8TH85

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR020719 RNA3'_term_phos_cycl-like_CS
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 3 hits
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit
PROSITEiView protein in PROSITE
PS01287 RTC, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TH85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIDGSYGE GGGQVVRTAV ALSAVTGKDV KVTNIRKNRP NPGLKQQHLK
60 70 80 90 100
ALETAARICE ARVSGLFPGS SELFFSPVEI KGGKYEVDIG TAGSITLLLQ
110 120 130 140 150
SLMPALPFAK EKVKLTIKGG TDVAWSPTID YLQHVTLRAL EQLGYAGIVT
160 170 180 190 200
LKKRGYYPRG GGEVSAVFEP CKLRSFHFRR VKENRRTGEA RREEETRNEG
210 220 230 240 250
RNQNQRIEIQ GISHASNLPA HVPARQAEAA ESLLLEAGYG SRTKIQSFEL
260 270 280 290 300
LSTGSGITLW SGYCGGSALG KKGLPAEKVG RRAAEEIILE LSSKAPVDVH
310 320 330 340 350
LADQLIPYMA LAGNSSYTVR ELTLHAATNI WVTEQFLDVK FRIEEKEGLF

EVSVD
Length:355
Mass (Da):38,735
Last modified:June 1, 2002 - v1
Checksum:i6E83259BA941176B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM07971.1
RefSeqiWP_011024505.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM07971; AAM07971; MA_4637
GeneIDi1476531
KEGGimac:MA_4637

Similar proteinsi

Entry informationi

Entry nameiRTCA_METAC
AccessioniPrimary (citable) accession number: Q8TH85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: June 1, 2002
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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