Nitrogenase iron protein - Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

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Q8TH41 (Q8TH41_METAC) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Nitrogenase iron protein HAMAP-Rule MF_00533
EC=1.18.6.1 HAMAP-Rule MF_00533

Alternative name(s):
Nitrogenase Fe protein HAMAP-Rule MF_00533
Nitrogenase component II HAMAP-Rule MF_00533
Nitrogenase reductase HAMAP-Rule MF_00533

Gene names

Name:	anfH EMBL AAM04624.1
Synonyms:	nifH HAMAP-Rule MF_00533, vnfH EMBL AAM04632.1
Ordered Locus Names:	MA_1205 EMBL AAM04624.1, MA_1213 EMBL AAM04632.1

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP] EMBL AAM04624.1

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina ›

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein By similarity. HAMAP-Rule MF_00533
Catalytic activity	8 reduced ferredoxin + 8 H⁺ + N₂ + 16 ATP + 16 H₂O = 8 oxidized ferredoxin + H₂ + 2 NH₃ + 16 ADP + 16 phosphate. HAMAP-Rule MF_00533
Cofactor	Binds 1 4Fe-4S cluster per dimer By similarity. HAMAP-Rule MF_00533
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00533
Post-translational modification	The reversible ADP-ribosylation of Arg-100 inactivates the nitrogenase reductase and regulates nitrogenase activity By similarity. HAMAP-Rule MF_00533
Sequence similarities	Belongs to the NifH/BchL/ChlL family. RuleBase RU003688 HAMAP-Rule MF_00533

Ontologies

Keywords
Biological process	Nitrogen fixation HAMAP-Rule MF_00533
Ligand	4Fe-4S RuleBase RU003688 HAMAP-Rule MF_00533 ATP-binding RuleBase RU003688 HAMAP-Rule MF_00533 Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase RuleBase RU003688 HAMAP-Rule MF_00533
PTM	ADP-ribosylation HAMAP-Rule MF_00533
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Cellular_component	molybdenum-iron nitrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP carbonyl sulfide nitrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

9 – 16

ATP By similarity HAMAP-Rule MF_00533

Sites

Metal binding

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity HAMAP-Rule MF_00533

Metal binding

132

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity HAMAP-Rule MF_00533

Amino acid modifications

Modified residue

100

ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase By similarity HAMAP-Rule MF_00533

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TH41 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 8165489942BBBF42
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FASTA

275

29,928

        10         20         30         40         50         60 
MTRKIAFYGK GGIGKSTTQQ NTAAAMAYYH GKNVFIHGCD PKADCTRLAL GGVPQTTIMD 

        70         80         90        100        110        120 
TLRELGEEAV TVDNVVNTGF KGIRCVESGG PEPGVGCAGR GVITAINLME ELGAYSDDLD 

       130        140        150        160        170        180 
FVHFDVLGDV VCGGFAMPIR EGKAQEVYIV ASGEMMATYA ANNICKGLLK YAEQSGVRLG 

       190        200        210        220        230        240 
GIICNSRKVD NELEMMEEFV SALGTQLIHF VPRDNIVQKA EFNKKTVVEF DPECNQAKEY 

       250        260        270 
GKLAKKILEN DMFVIPKPLS MDQLEKMVAR YGLMD

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References

[1]

"The genome of M. acetivorans reveals extensive metabolic and physiological diversity."
Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.

Birren B.
Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A and C2A EMBL AAM04624.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM04624.1. AE010299 Genomic DNA. Translation: AAM04632.1.
RefSeq	NP_616144.1. NC_003552.1. NP_616152.1. NC_003552.1.
3D structure databases
SMR	Q8TH41. Positions 3-272.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA1213.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM04624; AAM04624; MA_1205. AAM04632; AAM04632; MA_1213.
GeneID	1473093. 1473101.
KEGG	mac:MA1205. mac:MA1213.
Phylogenomic databases
eggNOG	COG1348.
KO	K02588.
OMA	CNQAMEY.
ProtClustDB	PRK13233.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-1217-MONOMER. MACE188937:GI2O-1225-MONOMER.
Family and domain databases
HAMAP	MF_00533. NifH.
InterPro	IPR000392. Nitogenase_NifH/Reductase_ChlL. IPR005977. Nitrogenase_Fe_NifH. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF00142. Fer4_NifH. 1 hit. [Graphical view]
PIRSF	PIRSF000363. Nitrogenase_iron. 1 hit.
PRINTS	PR00091. NITROGNASEII.
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01287. nifH. 1 hit.
PROSITE	PS00746. NIFH_FRXC_1. 1 hit. PS00692. NIFH_FRXC_2. 1 hit. PS51026. NIFH_FRXC_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q8TH41_METAC

Accession

Primary (citable) accession number: Q8TH41

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2002
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information