Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CCA-adding enzyme

Gene

cca

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATP or CTPUniRule annotation1
Binding sitei56ATP or CTPUniRule annotation1
Metal bindingi65MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi119MagnesiumUniRule annotation1
Binding sitei142ATP or CTPUniRule annotation1
Binding sitei161ATP or CTPUniRule annotation1
Binding sitei170ATP or CTPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:PF0026
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001390781 – 453CCA-adding enzymeAdd BLAST453

Proteomic databases

PRIDEiQ8TH22.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ8TH22. 1 interactor.
STRINGi186497.PF0026.

Structurei

3D structure databases

ProteinModelPortaliQ8TH22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TH22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIEEVIEEA LQKIVPKEEE ERFVKTLMSE IEEKARETIE ELNLNAKPYF
60 70 80 90 100
VGSLAKNTYL AGDHDLDLFI AFPLDTSLEK LREKGLEAGK VLGKKLGRYE
110 120 130 140 150
LAYAEHPYVR AEYKGVKVDI VPCYDVKSWK DVRTAVDRSI LHTKWVIENL
160 170 180 190 200
KGKNNEVRLF KRFLKGIKAY GSEIYVRGFS GYLAEILVIE FGSFLSVLEK
210 220 230 240 250
ADFMLKKKII DTGKWMKKES EITMKTIKRE AEEDKPLIVI DPVDPRRNVA
260 270 280 290 300
ANLSWERFGL FYFSSMQFLE KPSLEFFFPG EKRGNYREEL KRKGTHLVTL
310 320 330 340 350
LFTPPKLVDD LLLPQVEKTA KGLAKALEIE GFKVFGIDYG RNFIFLEVDR
360 370 380 390 400
VEKPRIEIKR GPLYFTSHGR RFYEKNEKVW IEGKDLMAEK KVGGFIVEVL
410 420 430 440 450
EEIFKKGQFS AGKNVKEVIK SSDILIDFVP KPLANEAYLF LSREKFNVKR

STS
Length:453
Mass (Da):52,447
Last modified:June 1, 2002 - v1
Checksum:i858955F2683F05F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80150.1.
RefSeqiWP_011011138.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80150; AAL80150; PF0026.
GeneIDi1467855.
KEGGipfu:PF0026.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL80150.1.
RefSeqiWP_011011138.1. NC_003413.1.

3D structure databases

ProteinModelPortaliQ8TH22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8TH22. 1 interactor.
STRINGi186497.PF0026.

Proteomic databases

PRIDEiQ8TH22.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80150; AAL80150; PF0026.
GeneIDi1467855.
KEGGipfu:PF0026.

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCCA_PYRFU
AccessioniPrimary (citable) accession number: Q8TH22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: June 1, 2002
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.