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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115UniRule annotation1
Active sitei123UniRule annotation1
Active sitei154UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:MK0341
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094711 – 179tRNA-splicing endonucleaseAdd BLAST179

Interactioni

Subunit structurei

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0341

Structurei

Secondary structure

1179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 17Combined sources5
Beta strandi20 – 23Combined sources4
Helixi28 – 32Combined sources5
Beta strandi36 – 39Combined sources4
Beta strandi42 – 45Combined sources4
Helixi47 – 55Combined sources9
Beta strandi60 – 62Combined sources3
Helixi71 – 79Combined sources9
Helixi85 – 97Combined sources13
Beta strandi101 – 104Combined sources4
Helixi106 – 108Combined sources3
Beta strandi110 – 117Combined sources8
Turni119 – 121Combined sources3
Beta strandi126 – 131Combined sources6
Helixi139 – 151Combined sources13
Beta strandi155 – 163Combined sources9
Turni164 – 167Combined sources4
Beta strandi168 – 177Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5X89X-ray1.53A2-179[»]
ProteinModelPortaliQ8TGZ7
SMRiQ8TGZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
HOGENOMiHOG000107823
KOiK01170
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01833 EndA_short, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR016442 tRNA_splic_arch_short
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF005285 tRNA_splic_archaea, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
SSF55267 SSF55267, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TGZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLCAGNGGKE LPRAKVFEGG SLVSKDYEDL KRRYFGTEHG NVLFLDPFET
60 70 80 90 100
VYLTEKGEID PETPEGEPMS VEELLSFFER RRPGFRAGYV VYRDLTERGY
110 120 130 140 150
VVKSGFKYGG RFRVYEEDPD REHSKYVVRV VEPDTELSTR DVLRATRLAH
160 170
SVRKDFVLAV VEDVEEPRIE YVMWRWKRL
Length:179
Mass (Da):20,939
Last modified:June 1, 2002 - v1
Checksum:iAC84893E8167E5D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM01556.1

Genome annotation databases

EnsemblBacteriaiAAM01556; AAM01556; MK0341
KEGGimka:MK0341

Entry informationi

Entry nameiENDA_METKA
AccessioniPrimary (citable) accession number: Q8TGZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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