Q8TGX9 (DUT_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	MA_0440

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00635
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00635
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00635
Sequence similarities	Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00635		PRO_0000153637

Sequences

	Sequence		Length	Mass (Da)
	Q8TGX9 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 645B29D302AE49E8	FASTA	171	19,259
	10 20 30 40 50 60 MTLLSSNELR KLIQATPHLL ENAVDIETQI QPNGLELTLK EIKTIEGVGA VDFDNSERKV 70 80 90 100 110 120 PDAKPLEFED DDWIHLPKGI YKVIFNEIVN IPMNLAAIAK PRSSLIRCGA TLETAVWDAG 130 140 150 160 170 YRGRSESMLV VYNSAGFRLK KNARIMQLLF YTLNSEVEEG YSGVYQNENT K

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM03887.1.
RefSeq	NP_615407.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TGX9.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA0440.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM03887; AAM03887; MA_0440.
GeneID	1472332.
KEGG	mac:MA0440.
Phylogenomic databases
eggNOG	COG0717.
KO	K01520.
OMA	WDAGYEG.
ProtClustDB	PRK02253.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-507-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00635. dUTPase_arch.
InterPro	IPR008180. dUTP_pyroPase. IPR023537. dUTP_pyroPase_arch. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUT_METAC

Accession

Primary (citable) accession number: Q8TGX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program