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Q8TGX1

- ENDA_METAC

UniProt

Q8TGX1 - ENDA_METAC

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei286 – 2861UniRule annotation
Active sitei297 – 2971UniRule annotation
Active sitei328 – 3281UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3667-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:MA_3624
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350tRNA-splicing endonucleasePRO_0000109487Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA3624.

Structurei

3D structure databases

ProteinModelPortaliQ8TGX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
InParanoidiQ8TGX1.
KOiK01170.
OMAiRGFYLSP.
PhylomeDBiQ8TGX1.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8TGX1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTQLEGDRV LAGKEAVAEL YKTGYFGRPR EDGLELSLVE AAYLQFRGKI
60 70 80 90 100
EIELEGRKLD FRALFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR
110 120 130 140 150
VYPRGSHPGK SAAKIFVHVL SERQPLPVKL LQDSVISAEN VHKQFILAVV
160 170 180 190 200
DEESDLTFYE IKTASPQGEM PEPYPEVKTD ATFLEDRVIA WDAEASGALY
210 220 230 240 250
AGGFYGKMLD PERLQLSLVE SLYLFSRGII VVRDRKDRIF SFDEFVEKAS
260 270 280 290 300
EIESSFLRKY GAYKALRDSG HVVKTGFKFG THFRVYRKVE SIEKIPHSEY
310 320 330 340 350
LVNVIPSDYE FRLPVMSGAV RLANSVRKRM LFAVEKEEGV EYLDISRVKM
Length:350
Mass (Da):40,133
Last modified:June 1, 2002 - v1
Checksum:i472FF8999A2C4C44
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM06979.1.
RefSeqiNP_618499.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06979; AAM06979; MA_3624.
GeneIDi1475517.
KEGGimac:MA3624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM06979.1 .
RefSeqi NP_618499.1. NC_003552.1.

3D structure databases

ProteinModelPortali Q8TGX1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 188937.MA3624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM06979 ; AAM06979 ; MA_3624 .
GeneIDi 1475517.
KEGGi mac:MA3624.

Phylogenomic databases

eggNOGi COG1676.
InParanoidi Q8TGX1.
KOi K01170.
OMAi RGFYLSP.
PhylomeDBi Q8TGX1.

Enzyme and pathway databases

BioCyci MACE188937:GI2O-3667-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
    Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
    , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
    Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Entry informationi

Entry nameiENDA_METAC
AccessioniPrimary (citable) accession number: Q8TGX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3