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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei286UniRule annotation1
Active sitei297UniRule annotation1
Active sitei328UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-3846-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:MA_3624
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094871 – 350tRNA-splicing endonucleaseAdd BLAST350

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA3624

Structurei

3D structure databases

ProteinModelPortaliQ8TGX1
SMRiQ8TGX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
InParanoidiQ8TGX1
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC
PhylomeDBiQ8TGX1

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 2 hits
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 2 hits

Sequencei

Sequence statusi: Complete.

Q8TGX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTQLEGDRV LAGKEAVAEL YKTGYFGRPR EDGLELSLVE AAYLQFRGKI
60 70 80 90 100
EIELEGRKLD FRALFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR
110 120 130 140 150
VYPRGSHPGK SAAKIFVHVL SERQPLPVKL LQDSVISAEN VHKQFILAVV
160 170 180 190 200
DEESDLTFYE IKTASPQGEM PEPYPEVKTD ATFLEDRVIA WDAEASGALY
210 220 230 240 250
AGGFYGKMLD PERLQLSLVE SLYLFSRGII VVRDRKDRIF SFDEFVEKAS
260 270 280 290 300
EIESSFLRKY GAYKALRDSG HVVKTGFKFG THFRVYRKVE SIEKIPHSEY
310 320 330 340 350
LVNVIPSDYE FRLPVMSGAV RLANSVRKRM LFAVEKEEGV EYLDISRVKM
Length:350
Mass (Da):40,133
Last modified:June 1, 2002 - v1
Checksum:i472FF8999A2C4C44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM06979.1
RefSeqiWP_011023532.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM06979; AAM06979; MA_3624
GeneIDi1475517
KEGGimac:MA_3624

Similar proteinsi

Entry informationi

Entry nameiENDA_METAC
AccessioniPrimary (citable) accession number: Q8TGX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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