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Q8TGX1 (ENDA_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:MA_3624
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000109487

Sites

Active site2861 By similarity
Active site2971 By similarity
Active site3281 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8TGX1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 472FF8999A2C4C44

FASTA35040,133
        10         20         30         40         50         60 
MKTQLEGDRV LAGKEAVAEL YKTGYFGRPR EDGLELSLVE AAYLQFRGKI EIELEGRKLD 

        70         80         90        100        110        120 
FRALFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR VYPRGSHPGK SAAKIFVHVL 

       130        140        150        160        170        180 
SERQPLPVKL LQDSVISAEN VHKQFILAVV DEESDLTFYE IKTASPQGEM PEPYPEVKTD 

       190        200        210        220        230        240 
ATFLEDRVIA WDAEASGALY AGGFYGKMLD PERLQLSLVE SLYLFSRGII VVRDRKDRIF 

       250        260        270        280        290        300 
SFDEFVEKAS EIESSFLRKY GAYKALRDSG HVVKTGFKFG THFRVYRKVE SIEKIPHSEY 

       310        320        330        340        350 
LVNVIPSDYE FRLPVMSGAV RLANSVRKRM LFAVEKEEGV EYLDISRVKM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE010299 Genomic DNA. Translation: AAM06979.1.
RefSeqNP_618499.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ8TGX1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING188937.MA3624.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM06979; AAM06979; MA_3624.
GeneID1475517.
KEGGmac:MA3624.

Phylogenomic databases

eggNOGCOG1676.
KOK01170.
OMARGFYLSP.
PhylomeDBQ8TGX1.

Enzyme and pathway databases

BioCycMACE188937:GI2O-3667-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 2 hits.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsTIGR00324. endA. 2 hits.
ProtoNetSearch...

Entry information

Entry nameENDA_METAC
AccessionPrimary (citable) accession number: Q8TGX1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families