Q8TGX1 (ENDA_METAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	MA_3624

Organism

Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]

Taxonomic identifier

188937 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › Methanosarcina acetivorans

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01834
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	tRNA-splicing endonuclease HAMAP-Rule MF_01834		PRO_0000109487
Sites
	Active site	286	1	By similarity
	Active site	297	1	By similarity
	Active site	328	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TGX1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 472FF8999A2C4C44	FASTA	350	40,133
	10 20 30 40 50 60 MKTQLEGDRV LAGKEAVAEL YKTGYFGRPR EDGLELSLVE AAYLQFRGKI EIELEGRKLD 70 80 90 100 110 120 FRALFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR VYPRGSHPGK SAAKIFVHVL 130 140 150 160 170 180 SERQPLPVKL LQDSVISAEN VHKQFILAVV DEESDLTFYE IKTASPQGEM PEPYPEVKTD 190 200 210 220 230 240 ATFLEDRVIA WDAEASGALY AGGFYGKMLD PERLQLSLVE SLYLFSRGII VVRDRKDRIF 250 260 270 280 290 300 SFDEFVEKAS EIESSFLRKY GAYKALRDSG HVVKTGFKFG THFRVYRKVE SIEKIPHSEY 310 320 330 340 350 LVNVIPSDYE FRLPVMSGAV RLANSVRKRM LFAVEKEEGV EYLDISRVKM

References

[1]

"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE010299 Genomic DNA. Translation: AAM06979.1.
RefSeq	NP_618499.1. NC_003552.1.
3D structure databases
ProteinModelPortal	Q8TGX1.
ModBase	Search...
Protein-protein interaction databases
STRING	188937.MA3624.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM06979; AAM06979; MA_3624.
GeneID	1475517.
KEGG	mac:MA3624.
Phylogenomic databases
eggNOG	COG1676.
KO	K01170.
OMA	FELKYIV.
ProtClustDB	PRK09300.
Enzyme and pathway databases
BioCyc	MACE188937:GI2O-3689-MONOMER.
Family and domain databases
Gene3D	3.40.1350.10. 2 hits.
HAMAP	MF_01834. EndA_long.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR023516. tRNA_splic_arch_long. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 2 hits. PF02778. tRNA_int_endo_N. 2 hits. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 2 hits. SSF55267. tRNA_int_endo_N. 2 hits.
TIGRFAMs	TIGR00324. endA. 2 hits.
ProtoNet	Search...

Entry information

Entry name

ENDA_METAC

Accession

Primary (citable) accession number: Q8TGX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program