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Q8TGX1

- ENDA_METAC

UniProt

Q8TGX1 - ENDA_METAC

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Protein
tRNA-splicing endonuclease
Gene
endA, MA_3624
Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei286 – 2861 By similarity
Active sitei297 – 2971 By similarity
Active sitei328 – 3281 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMACE188937:GI2O-3667-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:MA_3624
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000002487: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350tRNA-splicing endonucleaseUniRule annotation
PRO_0000109487Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi188937.MA3624.

Structurei

3D structure databases

ProteinModelPortaliQ8TGX1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
KOiK01170.
OMAiRGFYLSP.
PhylomeDBiQ8TGX1.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8TGX1-1 [UniParc]FASTAAdd to Basket

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MKTQLEGDRV LAGKEAVAEL YKTGYFGRPR EDGLELSLVE AAYLQFRGKI    50
EIELEGRKLD FRALFEQASL RQPNFELKYI VYKDLKERGY YVQPSAADFR 100
VYPRGSHPGK SAAKIFVHVL SERQPLPVKL LQDSVISAEN VHKQFILAVV 150
DEESDLTFYE IKTASPQGEM PEPYPEVKTD ATFLEDRVIA WDAEASGALY 200
AGGFYGKMLD PERLQLSLVE SLYLFSRGII VVRDRKDRIF SFDEFVEKAS 250
EIESSFLRKY GAYKALRDSG HVVKTGFKFG THFRVYRKVE SIEKIPHSEY 300
LVNVIPSDYE FRLPVMSGAV RLANSVRKRM LFAVEKEEGV EYLDISRVKM 350
Length:350
Mass (Da):40,133
Last modified:June 1, 2002 - v1
Checksum:i472FF8999A2C4C44
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM06979.1.
RefSeqiNP_618499.1. NC_003552.1.

Genome annotation databases

EnsemblBacteriaiAAM06979; AAM06979; MA_3624.
GeneIDi1475517.
KEGGimac:MA3624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE010299 Genomic DNA. Translation: AAM06979.1 .
RefSeqi NP_618499.1. NC_003552.1.

3D structure databases

ProteinModelPortali Q8TGX1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 188937.MA3624.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM06979 ; AAM06979 ; MA_3624 .
GeneIDi 1475517.
KEGGi mac:MA3624.

Phylogenomic databases

eggNOGi COG1676.
KOi K01170.
OMAi RGFYLSP.
PhylomeDBi Q8TGX1.

Enzyme and pathway databases

BioCyci MACE188937:GI2O-3667-MONOMER.