Reviewed, UniProtKB/Swiss-Prot Q8TGI1 (ATG1_PICPA)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATG1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1
    Glucose-induced selective autophagy protein 10
    Pexophagy zeocin-resistant mutant protein 1

Gene names

Name:	ATG1
Synonyms:	GSA10, PAZ1

Organism

Pichia pastoris (Yeast)

Taxonomic identifier

4922 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine-protein kinase required for pexophagy and autophagy and probably involved in cytoplasm to vacuole transport (Cvt). Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	Serine/threonine-protein kinase ATG1		PRO_0000085651
Regions
	Domain	9 – 304	296	Protein kinase
	Nucleotide binding	15 – 23	9	ATP By similarity
Sites
	Active site	155	1	Proton acceptor By similarity
	Binding site	38	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TGI1-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 3CFAAE3FE61413FA	FASTA	796	90,050
	10 20 30 40 50 60 MPDRKIGDYV VGAEIGRGSF ANVYKGYNSK TQVSVAIKSV IKSRLRNKKL IENLEVEISI 70 80 90 100 110 120 LKNLKHPHVV ALLDCEQSKH YFHLLMEYCS LGDLSYFITK REELISNHPL ITGVFKKYPS 130 140 150 160 170 180 PENSKGLNEV ITINFVQQLA SALKFLRSQN LVHRDIKPQN LLLSPPVSRE VFEDRKYTGL 190 200 210 220 230 240 WELPVLKIAD FGFARFLPAT SMAETLCGSP LYMAPEILRY EKYNAKADLW SVGAVVYEMS 250 260 270 280 290 300 VGTPPFPAHN HVELLRNIER QKDKISFPKV AQVPPEIIQL ICGLLKQQAT ERMSFQEFFN 310 320 330 340 350 360 DPVITTKLQP CSDEPLLPQN QHIDENLFIS EYLPRNSITD KNINNNITNI AKNGVEEALL 370 380 390 400 410 420 EEEDEEEDQD QLPSKNDNIQ HMEPDSSMLL NKTTQKQTEV QSQPRRELVS EKDYVVVEKR 430 440 450 460 470 480 AVEVNALADE LEHAGSGALA MQLTNNVGTP YTRRYSSSSR SSSTGSNQRR PSFGDRKVPI 490 500 510 520 530 540 SISPTNALSK AINIASNRLF KQPSPPKATP VLLEEKDKDK NTERLTSTVF NQNMLNSTTT 550 560 570 580 590 600 REITRPLHSL TITSSTSDEE IIQRLSNLTT KAYAIKLLAE IKFSQLAPLP PSNETAVFDN 610 620 630 640 650 660 YGDDEGTQSG YNNEPLSPIL IKTIGEEGIA LYVKTLFLLS KAMNIAMEWW RLNSLSRPAS 670 680 690 700 710 720 PKLNDLVQWI RGKFNESLEK AEFIKLKLQN AKEQLEESES DTDKTVVAEK LIFDRAIEIS 730 740 750 760 770 780 RIAVVNELKN DDLVGTELSY ATAIWMLEAL LEPDDTEESK LDDEDRKMIE KFISSIGNRL 790 SVLRKKIEST SQETRK

References

[1]	"GSA11 encodes a unique 208-kDa protein required for pexophagy and autophagy in Pichia pastoris." Stromhaug P.E., Bevan A., Dunn W.A. Jr. J. Biol. Chem. 276:42422-42435(2001) [PubMed: 11533052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY075104 Genomic DNA. Translation: AAL77195.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.1. 390.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR020656. Ser/Thr_prot_kinase_ATG1. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF39. Ser/Thr_prot_kinase_ATG1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_PICPA

Accession

Primary (citable) accession number: Q8TGI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2002
Last modified:	November 24, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)