Reviewed, UniProtKB/Swiss-Prot Q8TGH6 (GDA1_CANAL)

Names and origin

Protein names

Recommended name:
    Guanosine-diphosphatase
      Short name=GDPase
    EC=3.6.1.42

Gene names

Name:	GDA1
ORF Names:	CaO19.7394

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol. Ref.1 Has a role in cell wall morphogenesis during the transition of budding growth into hyphal growth, a process known as dimorphism. Ref.1
Catalytic activity	GDP + H2O = GMP + phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Ref.1
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	guanosine-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Guanosine-diphosphatase		PRO_0000209916
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 24	18	Signal-anchor for type II membrane protein Potential
	Topological domain	25 – 599	575	Lumenal Potential
	Compositional bias	52 – 56	5	Poly-Ala
	Compositional bias	125 – 129	5	Poly-Gln

Sequences

	Sequence		Length	Mass (Da)
	Q8TGH6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D5BC3A4F6E1B646A	FASTA	599	65,953
	10 20 30 40 50 60 MINPRNLRLI IAVIGLVGIF AFFASSQHSL VDRTALQVNK PAADSPHVPP PAAAAAPVSP 70 80 90 100 110 120 PPPSQQQKQQ EGSQKQLNDE NSDEKNTLQG TSYSGTKPPY EKVDGKSNKI LADKLDTTKN 130 140 150 160 170 180 SKPNQQQQQN TQKGASEEKS AQNKITTPEE VSMDNGKCND IDYVVMIDAG STGSRVHVYE 190 200 210 220 230 240 FNTCVKPPQL LSEEFEMLKP GLSSFDTDTV GAAKSLDPLL EVALKKVPKN KQSCTPVAVK 250 260 270 280 290 300 ATAGLRLLGE TKSKAILDEV RSHLEKDYPF AVVSEDGISI MDGKDEGVYA WVTANYLLGN 310 320 330 340 350 360 IGGKEKLPTA AVFDLGGGST QIVFEPDYKV DEVPVDGETK YHFTFGDNQY TLYQFSHLGY 370 380 390 400 410 420 GLMQGRNKVN QLVLKNKLSE LNLQKYTKKE VKGAKATVDV SNPCIPPGVV AKDVQVELGE 430 440 450 460 470 480 DEFYVVNMKG PSSKDSTVAG GSQCRYLAEK VLNKDAECTS KPCSFNGVHQ PSLTRTFNKN 490 500 510 520 530 540 SDMYVFSYFY DRTNPIGMPS SFSVEELKDL SKLVCQGETF WKDILLDDHV KNLNEEPQWC 550 560 570 580 590 LDLSFITAML HTGYDIPLHR ELKTAKTIDN NELGWCLGAS LPLLDKNNAK WTCRIDKTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall properties." Herrero A.B., Uccelletti D., Hirschberg C.B., Dominguez A., Abeijon C. Eukaryot. Cell 1:420-431(2002) [PubMed: 12455990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ421721 Genomic DNA. Translation: CAD18870.1. AACQ01000066 Genomic DNA. Translation: EAK97640.1.
RefSeq	XP_716635.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3641762.
KEGG	cal:CaO19.7394.
Organism-specific databases
CGD	CAL0004837. GDA1.
Phylogenomic databases
OMA	YLLGNIG.
Enzyme and pathway databases
BRENDA	3.6.1.42. 1124.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. IPR015605. Guanosine_diphosphatase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit. PTHR11782:SF9. Gda1p. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDA1_CANAL

Accession

Primary (citable) accession number: Q8TGH6
Secondary accession number(s): Q5A4E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)