Q8TGH6 (GDA1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanosine-diphosphatase
Short name=GDPase
EC=3.6.1.42

Gene names

Name:	GDA1
ORF Names:	CaO19.7394

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol. Ref.1 Has a role in cell wall morphogenesis during the transition of budding growth into hyphal growth, a process known as dimorphism. Ref.1
Catalytic activity	GDP + H2O = GMP + phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell growth mode switching, budding to filamentous Inferred from mutant phenotype Ref.1. Source: CGD cellular response to neutral pH Inferred from mutant phenotype Ref.1. Source: CGD filamentous growth of a population of unicellular organisms in response to neutral pH Inferred from mutant phenotype Ref.1. Source: CGD fungal-type cell wall biogenesis Inferred from mutant phenotype Ref.1. Source: CGD protein O-linked glycosylation Inferred from mutant phenotype Ref.1. Source: CGD
Cellular_component	Golgi membrane Inferred from direct assay Ref.1. Source: CGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	guanosine-diphosphatase activity Inferred from mutant phenotype Ref.1. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Guanosine-diphosphatase		PRO_0000209916
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 24	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	25 – 599	575	Lumenal Potential
	Compositional bias	52 – 56	5	Poly-Ala
	Compositional bias	125 – 129	5	Poly-Gln
Sites
	Active site	286	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TGH6 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: D5BC3A4F6E1B646A	FASTA	599	65,953
	10 20 30 40 50 60 MINPRNLRLI IAVIGLVGIF AFFASSQHSL VDRTALQVNK PAADSPHVPP PAAAAAPVSP 70 80 90 100 110 120 PPPSQQQKQQ EGSQKQLNDE NSDEKNTLQG TSYSGTKPPY EKVDGKSNKI LADKLDTTKN 130 140 150 160 170 180 SKPNQQQQQN TQKGASEEKS AQNKITTPEE VSMDNGKCND IDYVVMIDAG STGSRVHVYE 190 200 210 220 230 240 FNTCVKPPQL LSEEFEMLKP GLSSFDTDTV GAAKSLDPLL EVALKKVPKN KQSCTPVAVK 250 260 270 280 290 300 ATAGLRLLGE TKSKAILDEV RSHLEKDYPF AVVSEDGISI MDGKDEGVYA WVTANYLLGN 310 320 330 340 350 360 IGGKEKLPTA AVFDLGGGST QIVFEPDYKV DEVPVDGETK YHFTFGDNQY TLYQFSHLGY 370 380 390 400 410 420 GLMQGRNKVN QLVLKNKLSE LNLQKYTKKE VKGAKATVDV SNPCIPPGVV AKDVQVELGE 430 440 450 460 470 480 DEFYVVNMKG PSSKDSTVAG GSQCRYLAEK VLNKDAECTS KPCSFNGVHQ PSLTRTFNKN 490 500 510 520 530 540 SDMYVFSYFY DRTNPIGMPS SFSVEELKDL SKLVCQGETF WKDILLDDHV KNLNEEPQWC 550 560 570 580 590 LDLSFITAML HTGYDIPLHR ELKTAKTIDN NELGWCLGAS LPLLDKNNAK WTCRIDKTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall properties." Herrero A.B., Uccelletti D., Hirschberg C.B., Dominguez A., Abeijon C. Eukaryot. Cell 1:420-431(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ421721 Genomic DNA. Translation: CAD18870.1. AACQ01000066 Genomic DNA. Translation: EAK97640.1.
RefSeq	XP_716635.1. XM_711542.1.
3D structure databases
ProteinModelPortal	Q8TGH6.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0004837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3641762.
KEGG	cal:CaO19.7394.
Organism-specific databases
CGD	CAL0004837. GDA1.
Phylogenomic databases
eggNOG	COG5371.
KO	K01526.
Enzyme and pathway databases
BRENDA	3.6.1.42. 1096.
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. IPR015605. Guanosine_diphosphatase. [Graphical view]
PANTHER	PTHR11782. PTHR11782. 1 hit. PTHR11782:SF9. PTHR11782:SF9. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDA1_CANAL

Accession

Primary (citable) accession number: Q8TGH6
Secondary accession number(s): Q5A4E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program