Reviewed, UniProtKB/Swiss-Prot Q8TGG8 (GDA1_ASPFU)

Names and origin

Protein names

Recommended name:
    Probable guanosine-diphosphatase
      Short name=GDPase
    EC=3.6.1.42

Gene names

Name:	gda1
ORF Names:	AfA14E5.03c, AFUA_1G12150

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol By similarity.
Catalytic activity	GDP + H2O = GMP + phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	guanosine-diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Probable guanosine-diphosphatase		PRO_0000209915
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 26	18	Signal-anchor for type II membrane protein Potential
	Topological domain	27 – 503	477	Lumenal Potential
Amino acid modifications
	Glycosylation	318	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8TGG8-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: E49D642FA86CA04D	FASTA	503	55,250
	10 20 30 40 50 60 MAQTQRSRYL KTGAIVAALL LMLLWISPSR PMHPSFPQGQ PSTSAPAKGK CSKPHDPKKP 70 80 90 100 110 120 LIQYALMIDA GSQGSRIHVY RFNNCGPSPE LEDEVFFQTE PKKGGSGLSS YKEDAEGAAK 130 140 150 160 170 180 SLDPLMEVAM KNVPDEYKSC SPIAVKATAG LRMLGPELSQ KILDAVRNRL ETVYPFPVVS 190 200 210 220 230 240 KEKGGVEIMD GSDEGVYAWI TTNYLLGKIG GPDETPTAAV FDLGGGSTQI VFQPTFPKSK 250 260 270 280 290 300 SGGMPERLSE GDHKYDLQFG GRHFELYQHS HLGYGLMAAR KAIHTSIIEN MLASSPKDLS 310 320 330 340 350 360 WLKQPIPNPC IGPEMEKNVT LEFPEGHKLA PEVQVTMVGP KVGSTAAQCR GLAEKTLNKE 370 380 390 400 410 420 AACTLAPCSF NGVHQPSLEK TFAREDVYIF SYFYDRTKPL GMPDSFTLDE LHQLTSTVCG 430 440 450 460 470 480 GEDSWGIFAG IEGALKELRD RPEWCLDLNF MMSLLHTGYE MPLSREVKIA KKIKNRELGW 490 500 CLGASLPLLS QESGWTCRIK EVS

References

[1]

"Insight into the genome of Aspergillus fumigatus: analysis of a 922 kb region encompassing the nitrate assimilation gene cluster." Pain A., Woodward J.R., Quail M.A., Anderson M.J., Clark R., Collins M., Fosker N., Fraser A., Harris D.E., Larke N., Murphy L.D., Humphray S., O'Neil S., Pertea M., Price C., Rabbinowitsch E., Rajandream M.A., Salzberg S.L. , Saunders D., Seeger K., Sharp S., Warren T., Denning D.W., Barrell B.G., Hall N. Fungal Genet. Biol. 41:443-453(2004) [PubMed: 14998527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

[2]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL683874 Genomic DNA. Translation: CAD27295.1. Different initiation. BX649605 Genomic DNA. Translation: CAE47942.1. AAHF01000004 Genomic DNA. Translation: EAL90545.1.
RefSeq	XP_752583.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8TGG8.
Genome annotation databases
GeneID	3510633.
KEGG	afm:AFUA_1G12150.
Phylogenomic databases
eggNOG	fuNOG05233.
HOGENOM	HBG736908.
OMA	PRRVATE.
OrthoDB	EOG9FBK9C.
PhylomeDB	Q8TGG8.
Enzyme and pathway databases
BRENDA	3.6.1.42. 18841.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. IPR015605. Guanosine_diphosphatase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit. PTHR11782:SF9. Gda1p. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDA1_ASPFU

Accession

Primary (citable) accession number: Q8TGG8
Secondary accession number(s): Q4WSN7, Q6MYR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	December 6, 2005
Last modified:	February 9, 2010
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)