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Q8TGE1

- AWA1_YEASK

UniProt

Q8TGE1 - AWA1_YEASK

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Protein

Cell wall protein AWA1

Gene
AWA1, SYK7_061821
Organism
Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in cell wall organization and biosynthesis. Confers cell surface hydrophobicity (CSH).

GO - Biological processi

  1. response to stress Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein AWA1
Gene namesi
Name:AWA1
ORF Names:SYK7_061821
OrganismiSaccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
Taxonomic identifieri721032 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000001608: Chromosome XV

Organism-specific databases

SGDiS000029703. AWA1.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-SubCell
  3. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

Responsible for the thick foam layer formation on the sake mash during the fermentation process.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 16921669Cell wall protein AWA1PRO_0000268175Add
BLAST
Propeptidei1693 – 171321Removed in mature form Reviewed predictionPRO_0000268176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1133 – 11331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1241 – 12411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1278 – 12781N-linked (GlcNAc...) Reviewed prediction
Lipidationi1692 – 16921GPI-anchor amidated alanine Reviewed prediction

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8TGE1.

Expressioni

Gene expression databases

GenevestigatoriQ8TGE1.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 968941Ser-richAdd
BLAST
Compositional biasi1266 – 1659394Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG12793.
OrthoDBiEOG7VQJPN.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TGE1-1 [UniParc]FASTAAdd to Basket

« Hide

MFNRFNKLQA ALALVLYSQS ALGQYYTSSS IASNSSTAVS STSSGSVSIS     50
SSIELTSSTS DVSSSLTELT SSFTEVSSSI APSTSSSEVS SSITSSGSSV 100
SGSSSITSSG SSVSSSSPYD ERFNSLDLSV HVSAGFSAGV SVGLEPSATT 150
ASVTTTLSPY DERVNLIELG VYVSDMRAHL VEYLLFQAAH STEPHPTEIA 200
AAFLDHGDFT TRLTGISGDE VTRMITGVPW YSTRLKPAIS EALAKDGIYT 250
AIPTSTSTTS DTYISSSSPS QVTSSAEPTT VSGVTSSVEP TRSSQVTSSA 300
EPTTVSEITS SAEPLSSSKA TTSAESISSN QITISSELIV SSVITSSSEI 350
PSSIEVLTSS GISSSVEPTS LVGPSSDESI SSTESLSATS TPLAVSSTVV 400
TSSTDSVSPN IPFSEISSSP ESSTAITSGS SSATESGSSV SGSTSATESG 450
SSASGSSSAT ESGSSVSGST SATESGSASS VPSSSGSVTE SGSSSSASES 500
SITQSGTASG SSVSSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS 550
TASGSITSGT LTSITSGSSS ATESGSSVSG SSSATESGSS VSGSTSATES 600
GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSVSGSTSAT 650
ESGSSASGSS SATESGSASS VPSSSGSVTE SGSSSSASES SITQSGTASG 700
SSASSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS TASGSITSGT 750
LTSITSGSSS ATESGSSASG SSSATESGSS VSGSTSATES GSSVSGSTSA 800
TESGSSASGS SSATESGSSV SGSTSATESG SSASGSSSAT ESGSASSVPS 850
SSGSVTESGS SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS 900
STSGSVTQSG SSVSGSSASS APGISSSIPQ STSSASTASG SITSGTLTSI 950
TSSASSASAT ASNSLSSSDG TIYLPTTTIS GDLTLTGKVI ATEGVVVAAG 1000
AKLTLLDGDK YSFSADLKVY GDLLVKKSKE TYPGTEFDIS GENFDVTGNF 1050
NAEESAATSA SIYSFTPSSF DNSGDISLSL SKSKKGEVTF SPYSNSGAFS 1100
FSNAILNGGS VSGLQRRDDT EGSVNNGEIN LDNGSTYVIV EPVSGKGTVN 1150
IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTESNTTPIP VVGYTGENQI 1200
AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF NVSEGTFAGA 1250
YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG 1300
ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT 1350
TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN 1400
GCNTKTVTSE APEATTTTVS PKTYTTATVT QCDDNGCSTK TVTSECPEET 1450
SATTTSPKSY TTVTVTHCDD NGCNTKTVTS EAPEATTTTV SPKTYTTATV 1500
TQCDDNGCST KTVTSECPEE TSATTTSPKS YTTVTVTHCD DNGCNTKTVT 1550
SEAPEATTTT VSPKTYTTAT VTQCDDNGCS TKTVTSEAPK ETSETSETSA 1600
APKTYTTATV TQCDDNGCNV KIITSQIPEA TSTVTATSAS PKSYTTVTSE 1650
GSKATSLTTA ISKASSAIST YSKSAAPIKT STGIIVQSEG IAAGLNANTL 1700
NALVGIFVLA FFN 1713
Length:1,713
Mass (Da):166,884
Last modified:June 1, 2002 - v1
Checksum:i9321A296EB586404
GO

Polymorphismi

Half of the downstream region (residues 1035 to 1713) of the AWA1 gene was lost in the nonfoaming strain K701 due to a chromosomal recombination event.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1036 – 1713678Missing in strain: Kyokai no. 701, non-foaming mutant derivative of Kyokai no. 7.
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071164 Genomic DNA. Translation: BAB85832.1.
AB110100 Genomic DNA. Translation: BAD06576.1.
AB110101 Genomic DNA. Translation: BAD06577.1.
DG000051 Genomic DNA. Translation: GAA26176.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB071164 Genomic DNA. Translation: BAB85832.1 .
AB110100 Genomic DNA. Translation: BAD06576.1 .
AB110101 Genomic DNA. Translation: BAD06577.1 .
DG000051 Genomic DNA. Translation: GAA26176.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8TGE1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

SGDi S000029703. AWA1.

Phylogenomic databases

eggNOGi NOG12793.
OrthoDBi EOG7VQJPN.

Gene expression databases

Genevestigatori Q8TGE1.

Family and domain databases

InterProi IPR000992. SRP1_TIP1.
[Graphical view ]
Pfami PF00660. SRP1_TIP1. 1 hit.
[Graphical view ]
PROSITEi PS00724. SRP1_TIP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast."
    Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K.
    Appl. Environ. Microbiol. 68:2018-2025(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, BIOTECHNOLOGY.
    Strain: Kyokai no. 7 / NBRC 101557.
  2. "Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake Yeast."
    Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H.
    J. Biosci. Bioeng. 97:14-18(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Kyokai no. 7 / NBRC 101557 and Kyokai no. 701.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kyokai no. 7 / NBRC 101557.

Entry informationi

Entry nameiAWA1_YEASK
AccessioniPrimary (citable) accession number: Q8TGE1
Secondary accession number(s): G2WM83, Q76C74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

'Awa' means 'foam' in Japanese.

Keywords - Technical termi

Complete proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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