Reviewed,
UniProtKB/Swiss-Prot Q8TGE1 (AWA1_YEAST)
Last modified
November 24, 2009.
Version 35.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Cell wall protein AWA1 | ||
| Gene names |
| ||
| Organism | Saccharomyces cerevisiae (Baker's yeast) | ||
| Taxonomic identifier | 4932 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 1713 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in cell wall organization and biosynthesis. Confers cell surface hydrophobicity (CSH). |
| Subcellular location | Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.1 |
| Post-translational modification | The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved of and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer. |
| Polymorphism | Half of the downstream region (residues 1035 to 1713) of the AWA1 gene was lost in nonfoaming strain K701 due to a chromosomal recombination. |
| Biotechnological use | Responsible for the thick foam layer formation on the sake mash during the fermentation process. Ref.1 |
| Miscellaneous | 'Awa' means 'foam' in Japanese. |
| Sequence similarities | Belongs to the SRP1/TIP1 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell wall biogenesis/degradation |
| Cellular component | Cell wall Membrane Secreted |
| Domain | Signal |
| PTM | GPI-anchor Glycoprotein Lipoprotein |
| Gene Ontology (GO) | |
| Biological process | cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW response to stressInferred from electronic annotation. Source: InterPro |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell wallInferred from electronic annotation. Source: UniProtKB-SubCell extracellular regionInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 23 | 23 | Potential | ||||||
| Chain | 24 – 1692 | 1669 | Cell wall protein AWA1 | PRO_0000268175 | |||||
| Propeptide | 1693 – 1713 | 21 | Removed in mature form Potential | PRO_0000268176 | |||||
Regions | |||||||||
| Compositional bias | 28 – 968 | 941 | Ser-rich | ||||||
| Compositional bias | 1266 – 1659 | 394 | Thr-rich | ||||||
Amino acid modifications | |||||||||
| Lipidation | 1692 | 1 | GPI-anchor amidated alanine Potential | ||||||
| Glycosylation | 34 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1133 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1241 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1278 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Natural variant | 1036 – 1713 | 678 | Missing in strain: K701. | ||||||
Sequences
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References
| [1] | "The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast." Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K. Appl. Environ. Microbiol. 68:2018-2025(2002) [PubMed: 11916725] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, BIOTECHNOLOGY. Strain: K7. |
| [2] | "Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake Yeast." Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H. J. Biosci. Bioeng. 97:14-18(2004) [PubMed: 16233582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K701. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AB071164 Genomic DNA. Translation: BAB85832.1. AB110100 Genomic DNA. Translation: BAD06576.1. AB110101 Genomic DNA. Translation: BAD06577.1. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1SWO based on UniProtKB P22629. |
| ModBase | Search... |
Organism-specific databases | |
| SGD | S000029703. AWA1. |
Gene expression databases | |
| Genevestigator | Q8TGE1. |
Family and domain databases | |
| InterPro | IPR000992. SRP1_TIP1. [Graphical view] |
| Pfam | PF00660. SRP1_TIP1. 1 hit. [Graphical view] |
| PROSITE | PS00724. SRP1_TIP1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AWA1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q8TGE1 Secondary accession number(s): Q76C74 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Protein Spotlight Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
