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Q8TGE1 (AWA1_YEASK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell wall protein AWA1
Gene names
Name:AWA1
ORF Names:SYK7_061821
OrganismSaccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast) [Complete proteome]
Taxonomic identifier721032 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length1713 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell wall organization and biosynthesis. Confers cell surface hydrophobicity (CSH).

Subcellular location

Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.1

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Polymorphism

Half of the downstream region (residues 1035 to 1713) of the AWA1 gene was lost in the nonfoaming strain K701 due to a chromosomal recombination event.

Biotechnological use

Responsible for the thick foam layer formation on the sake mash during the fermentation process. Ref.1

Miscellaneous

'Awa' means 'foam' in Japanese.

Sequence similarities

Belongs to the SRP1/TIP1 family.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Membrane
Secreted
   DomainSignal
   PTMGPI-anchor
Glycoprotein
Lipoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processresponse to stress

Inferred from electronic annotation. Source: InterPro

   Cellular_componentanchored to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 16921669Cell wall protein AWA1
PRO_0000268175
Propeptide1693 – 171321Removed in mature form Potential
PRO_0000268176

Regions

Compositional bias28 – 968941Ser-rich
Compositional bias1266 – 1659394Thr-rich

Amino acid modifications

Lipidation16921GPI-anchor amidated alanine Potential
Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation11331N-linked (GlcNAc...) Potential
Glycosylation12411N-linked (GlcNAc...) Potential
Glycosylation12781N-linked (GlcNAc...) Potential

Natural variations

Natural variant1036 – 1713678Missing in strain: Kyokai no. 701, non-foaming mutant derivative of Kyokai no. 7.

Sequences

Sequence LengthMass (Da)Tools
Q8TGE1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 9321A296EB586404

FASTA1,713166,884
        10         20         30         40         50         60 
MFNRFNKLQA ALALVLYSQS ALGQYYTSSS IASNSSTAVS STSSGSVSIS SSIELTSSTS 

        70         80         90        100        110        120 
DVSSSLTELT SSFTEVSSSI APSTSSSEVS SSITSSGSSV SGSSSITSSG SSVSSSSPYD 

       130        140        150        160        170        180 
ERFNSLDLSV HVSAGFSAGV SVGLEPSATT ASVTTTLSPY DERVNLIELG VYVSDMRAHL 

       190        200        210        220        230        240 
VEYLLFQAAH STEPHPTEIA AAFLDHGDFT TRLTGISGDE VTRMITGVPW YSTRLKPAIS 

       250        260        270        280        290        300 
EALAKDGIYT AIPTSTSTTS DTYISSSSPS QVTSSAEPTT VSGVTSSVEP TRSSQVTSSA 

       310        320        330        340        350        360 
EPTTVSEITS SAEPLSSSKA TTSAESISSN QITISSELIV SSVITSSSEI PSSIEVLTSS 

       370        380        390        400        410        420 
GISSSVEPTS LVGPSSDESI SSTESLSATS TPLAVSSTVV TSSTDSVSPN IPFSEISSSP 

       430        440        450        460        470        480 
ESSTAITSGS SSATESGSSV SGSTSATESG SSASGSSSAT ESGSSVSGST SATESGSASS 

       490        500        510        520        530        540 
VPSSSGSVTE SGSSSSASES SITQSGTASG SSVSSTSGSV TQSGSSVSGS SASSAPGISS 

       550        560        570        580        590        600 
SIPQSTSSAS TASGSITSGT LTSITSGSSS ATESGSSVSG SSSATESGSS VSGSTSATES 

       610        620        630        640        650        660 
GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSVSGSTSAT ESGSSASGSS 

       670        680        690        700        710        720 
SATESGSASS VPSSSGSVTE SGSSSSASES SITQSGTASG SSASSTSGSV TQSGSSVSGS 

       730        740        750        760        770        780 
SASSAPGISS SIPQSTSSAS TASGSITSGT LTSITSGSSS ATESGSSASG SSSATESGSS 

       790        800        810        820        830        840 
VSGSTSATES GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSASGSSSAT 

       850        860        870        880        890        900 
ESGSASSVPS SSGSVTESGS SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS 

       910        920        930        940        950        960 
STSGSVTQSG SSVSGSSASS APGISSSIPQ STSSASTASG SITSGTLTSI TSSASSASAT 

       970        980        990       1000       1010       1020 
ASNSLSSSDG TIYLPTTTIS GDLTLTGKVI ATEGVVVAAG AKLTLLDGDK YSFSADLKVY 

      1030       1040       1050       1060       1070       1080 
GDLLVKKSKE TYPGTEFDIS GENFDVTGNF NAEESAATSA SIYSFTPSSF DNSGDISLSL 

      1090       1100       1110       1120       1130       1140 
SKSKKGEVTF SPYSNSGAFS FSNAILNGGS VSGLQRRDDT EGSVNNGEIN LDNGSTYVIV 

      1150       1160       1170       1180       1190       1200 
EPVSGKGTVN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTESNTTPIP VVGYTGENQI 

      1210       1220       1230       1240       1250       1260 
AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF NVSEGTFAGA YAYYLNYGGV 

      1270       1280       1290       1300       1310       1320 
VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG ASVTGSTAST LISGSPSVYT 

      1330       1340       1350       1360       1370       1380 
TTLTYATTTS TVVVSCSETT DSNGNVYTIT TTVPCSSTTA TITSCDETGC HVTTSTGTVA 

      1390       1400       1410       1420       1430       1440 
TETVSSKSYT TVTVTHCDNN GCNTKTVTSE APEATTTTVS PKTYTTATVT QCDDNGCSTK 

      1450       1460       1470       1480       1490       1500 
TVTSECPEET SATTTSPKSY TTVTVTHCDD NGCNTKTVTS EAPEATTTTV SPKTYTTATV 

      1510       1520       1530       1540       1550       1560 
TQCDDNGCST KTVTSECPEE TSATTTSPKS YTTVTVTHCD DNGCNTKTVT SEAPEATTTT 

      1570       1580       1590       1600       1610       1620 
VSPKTYTTAT VTQCDDNGCS TKTVTSEAPK ETSETSETSA APKTYTTATV TQCDDNGCNV 

      1630       1640       1650       1660       1670       1680 
KIITSQIPEA TSTVTATSAS PKSYTTVTSE GSKATSLTTA ISKASSAIST YSKSAAPIKT 

      1690       1700       1710 
STGIIVQSEG IAAGLNANTL NALVGIFVLA FFN

« Hide

References

« Hide 'large scale' references
[1]"The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast."
Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K.
Appl. Environ. Microbiol. 68:2018-2025(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, BIOTECHNOLOGY.
Strain: Kyokai no. 7 / NBRC 101557.
[2]"Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake Yeast."
Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H.
J. Biosci. Bioeng. 97:14-18(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Kyokai no. 7 / NBRC 101557 and Kyokai no. 701.
[3]"Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no. 7."
Akao T., Yashiro I., Hosoyama A., Kitagaki H., Horikawa H., Watanabe D., Akada R., Ando Y., Harashima S., Inoue T., Inoue Y., Kajiwara S., Kitamoto K., Kitamoto N., Kobayashi O., Kuhara S., Masubuchi T., Mizoguchi H. expand/collapse author list , Nakao Y., Nakazato A., Namise M., Oba T., Ogata T., Ohta A., Sato M., Shibasaki S., Takatsume Y., Tanimoto S., Tsuboi H., Nishimura A., Yoda K., Ishikawa T., Iwashita K., Fujita N., Shimoi H.
DNA Res. 18:423-434(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Kyokai no. 7 / NBRC 101557.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB071164 Genomic DNA. Translation: BAB85832.1.
AB110100 Genomic DNA. Translation: BAD06576.1.
AB110101 Genomic DNA. Translation: BAD06577.1.
DG000051 Genomic DNA. Translation: GAA26176.1.

3D structure databases

HSSPHSSP built from PDB template 1SWO based on UniProtKB P22629.
ModBaseSearch...

Proteomic databases

PaxDbQ8TGE1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

SGDS000029703. AWA1.

Phylogenomic databases

eggNOGNOG12793.

Gene expression databases

GenevestigatorQ8TGE1.

Family and domain databases

InterProIPR000992. SRP1_TIP1.
[Graphical view]
PfamPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEPS00724. SRP1_TIP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAWA1_YEASK
AccessionPrimary (citable) accession number: Q8TGE1
Secondary accession number(s): G2WM83, Q76C74
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2002
Last modified: April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents