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Q8TGE1

- AWA1_YEASK

UniProt

Q8TGE1 - AWA1_YEASK

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Protein

Cell wall protein AWA1

Gene

AWA1

Organism
Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in cell wall organization and biosynthesis. Confers cell surface hydrophobicity (CSH).

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. response to stress Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein AWA1
Gene namesi
Name:AWA1
ORF Names:SYK7_061821
OrganismiSaccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
Taxonomic identifieri721032 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000001608: Chromosome XV

Organism-specific databases

SGDiS000029703. AWA1.

Subcellular locationi

Secretedcell wall 1 Publication. Membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
  3. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

Responsible for the thick foam layer formation on the sake mash during the fermentation process.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 16921669Cell wall protein AWA1PRO_0000268175Add
BLAST
Propeptidei1693 – 171321Removed in mature formSequence AnalysisPRO_0000268176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1133 – 11331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1241 – 12411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1278 – 12781N-linked (GlcNAc...)Sequence Analysis
Lipidationi1692 – 16921GPI-anchor amidated alanineSequence Analysis

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ8TGE1.

Expressioni

Gene expression databases

GenevestigatoriQ8TGE1.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 968941Ser-richAdd
BLAST
Compositional biasi1266 – 1659394Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG12793.
OrthoDBiEOG7VQJPN.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8TGE1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNRFNKLQA ALALVLYSQS ALGQYYTSSS IASNSSTAVS STSSGSVSIS
60 70 80 90 100
SSIELTSSTS DVSSSLTELT SSFTEVSSSI APSTSSSEVS SSITSSGSSV
110 120 130 140 150
SGSSSITSSG SSVSSSSPYD ERFNSLDLSV HVSAGFSAGV SVGLEPSATT
160 170 180 190 200
ASVTTTLSPY DERVNLIELG VYVSDMRAHL VEYLLFQAAH STEPHPTEIA
210 220 230 240 250
AAFLDHGDFT TRLTGISGDE VTRMITGVPW YSTRLKPAIS EALAKDGIYT
260 270 280 290 300
AIPTSTSTTS DTYISSSSPS QVTSSAEPTT VSGVTSSVEP TRSSQVTSSA
310 320 330 340 350
EPTTVSEITS SAEPLSSSKA TTSAESISSN QITISSELIV SSVITSSSEI
360 370 380 390 400
PSSIEVLTSS GISSSVEPTS LVGPSSDESI SSTESLSATS TPLAVSSTVV
410 420 430 440 450
TSSTDSVSPN IPFSEISSSP ESSTAITSGS SSATESGSSV SGSTSATESG
460 470 480 490 500
SSASGSSSAT ESGSSVSGST SATESGSASS VPSSSGSVTE SGSSSSASES
510 520 530 540 550
SITQSGTASG SSVSSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS
560 570 580 590 600
TASGSITSGT LTSITSGSSS ATESGSSVSG SSSATESGSS VSGSTSATES
610 620 630 640 650
GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSVSGSTSAT
660 670 680 690 700
ESGSSASGSS SATESGSASS VPSSSGSVTE SGSSSSASES SITQSGTASG
710 720 730 740 750
SSASSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS TASGSITSGT
760 770 780 790 800
LTSITSGSSS ATESGSSASG SSSATESGSS VSGSTSATES GSSVSGSTSA
810 820 830 840 850
TESGSSASGS SSATESGSSV SGSTSATESG SSASGSSSAT ESGSASSVPS
860 870 880 890 900
SSGSVTESGS SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS
910 920 930 940 950
STSGSVTQSG SSVSGSSASS APGISSSIPQ STSSASTASG SITSGTLTSI
960 970 980 990 1000
TSSASSASAT ASNSLSSSDG TIYLPTTTIS GDLTLTGKVI ATEGVVVAAG
1010 1020 1030 1040 1050
AKLTLLDGDK YSFSADLKVY GDLLVKKSKE TYPGTEFDIS GENFDVTGNF
1060 1070 1080 1090 1100
NAEESAATSA SIYSFTPSSF DNSGDISLSL SKSKKGEVTF SPYSNSGAFS
1110 1120 1130 1140 1150
FSNAILNGGS VSGLQRRDDT EGSVNNGEIN LDNGSTYVIV EPVSGKGTVN
1160 1170 1180 1190 1200
IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTESNTTPIP VVGYTGENQI
1210 1220 1230 1240 1250
AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF NVSEGTFAGA
1260 1270 1280 1290 1300
YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG
1310 1320 1330 1340 1350
ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT
1360 1370 1380 1390 1400
TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN
1410 1420 1430 1440 1450
GCNTKTVTSE APEATTTTVS PKTYTTATVT QCDDNGCSTK TVTSECPEET
1460 1470 1480 1490 1500
SATTTSPKSY TTVTVTHCDD NGCNTKTVTS EAPEATTTTV SPKTYTTATV
1510 1520 1530 1540 1550
TQCDDNGCST KTVTSECPEE TSATTTSPKS YTTVTVTHCD DNGCNTKTVT
1560 1570 1580 1590 1600
SEAPEATTTT VSPKTYTTAT VTQCDDNGCS TKTVTSEAPK ETSETSETSA
1610 1620 1630 1640 1650
APKTYTTATV TQCDDNGCNV KIITSQIPEA TSTVTATSAS PKSYTTVTSE
1660 1670 1680 1690 1700
GSKATSLTTA ISKASSAIST YSKSAAPIKT STGIIVQSEG IAAGLNANTL
1710
NALVGIFVLA FFN
Length:1,713
Mass (Da):166,884
Last modified:June 1, 2002 - v1
Checksum:i9321A296EB586404
GO

Polymorphismi

Half of the downstream region (residues 1035 to 1713) of the AWA1 gene was lost in the nonfoaming strain K701 due to a chromosomal recombination event.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1036 – 1713678Missing in strain: Kyokai no. 701, non-foaming mutant derivative of Kyokai no. 7.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071164 Genomic DNA. Translation: BAB85832.1.
AB110100 Genomic DNA. Translation: BAD06576.1.
AB110101 Genomic DNA. Translation: BAD06577.1.
DG000051 Genomic DNA. Translation: GAA26176.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071164 Genomic DNA. Translation: BAB85832.1 .
AB110100 Genomic DNA. Translation: BAD06576.1 .
AB110101 Genomic DNA. Translation: BAD06577.1 .
DG000051 Genomic DNA. Translation: GAA26176.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q8TGE1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

SGDi S000029703. AWA1.

Phylogenomic databases

eggNOGi NOG12793.
OrthoDBi EOG7VQJPN.

Gene expression databases

Genevestigatori Q8TGE1.

Family and domain databases

InterProi IPR000992. SRP1_TIP1.
[Graphical view ]
Pfami PF00660. SRP1_TIP1. 1 hit.
[Graphical view ]
PROSITEi PS00724. SRP1_TIP1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast."
    Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K.
    Appl. Environ. Microbiol. 68:2018-2025(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, BIOTECHNOLOGY.
    Strain: Kyokai no. 7 / NBRC 101557.
  2. "Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake Yeast."
    Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H.
    J. Biosci. Bioeng. 97:14-18(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Kyokai no. 7 / NBRC 101557 and Kyokai no. 701.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Kyokai no. 7 / NBRC 101557.

Entry informationi

Entry nameiAWA1_YEASK
AccessioniPrimary (citable) accession number: Q8TGE1
Secondary accession number(s): G2WM83, Q76C74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

'Awa' means 'foam' in Japanese.

Keywords - Technical termi

Complete proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3