Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8TGE1

- AWA1_YEASK

UniProt

Q8TGE1 - AWA1_YEASK

Protein

Cell wall protein AWA1

Gene

AWA1

Organism
Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in cell wall organization and biosynthesis. Confers cell surface hydrophobicity (CSH).

    GO - Biological processi

    1. response to stress Source: InterPro

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell wall protein AWA1
    Gene namesi
    Name:AWA1
    ORF Names:SYK7_061821
    OrganismiSaccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast)
    Taxonomic identifieri721032 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000001608: Chromosome XV

    Organism-specific databases

    SGDiS000029703. AWA1.

    Subcellular locationi

    Secretedcell wall 1 Publication. Membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cell wall Source: UniProtKB-SubCell
    3. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    Pathology & Biotechi

    Biotechnological usei

    Responsible for the thick foam layer formation on the sake mash during the fermentation process.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 16921669Cell wall protein AWA1PRO_0000268175Add
    BLAST
    Propeptidei1693 – 171321Removed in mature formSequence AnalysisPRO_0000268176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1133 – 11331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1241 – 12411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1278 – 12781N-linked (GlcNAc...)Sequence Analysis
    Lipidationi1692 – 16921GPI-anchor amidated alanineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ8TGE1.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8TGE1.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi28 – 968941Ser-richAdd
    BLAST
    Compositional biasi1266 – 1659394Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SRP1/TIP1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    OrthoDBiEOG7VQJPN.

    Family and domain databases

    InterProiIPR000992. SRP1_TIP1.
    [Graphical view]
    PfamiPF00660. SRP1_TIP1. 1 hit.
    [Graphical view]
    PROSITEiPS00724. SRP1_TIP1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8TGE1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNRFNKLQA ALALVLYSQS ALGQYYTSSS IASNSSTAVS STSSGSVSIS     50
    SSIELTSSTS DVSSSLTELT SSFTEVSSSI APSTSSSEVS SSITSSGSSV 100
    SGSSSITSSG SSVSSSSPYD ERFNSLDLSV HVSAGFSAGV SVGLEPSATT 150
    ASVTTTLSPY DERVNLIELG VYVSDMRAHL VEYLLFQAAH STEPHPTEIA 200
    AAFLDHGDFT TRLTGISGDE VTRMITGVPW YSTRLKPAIS EALAKDGIYT 250
    AIPTSTSTTS DTYISSSSPS QVTSSAEPTT VSGVTSSVEP TRSSQVTSSA 300
    EPTTVSEITS SAEPLSSSKA TTSAESISSN QITISSELIV SSVITSSSEI 350
    PSSIEVLTSS GISSSVEPTS LVGPSSDESI SSTESLSATS TPLAVSSTVV 400
    TSSTDSVSPN IPFSEISSSP ESSTAITSGS SSATESGSSV SGSTSATESG 450
    SSASGSSSAT ESGSSVSGST SATESGSASS VPSSSGSVTE SGSSSSASES 500
    SITQSGTASG SSVSSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS 550
    TASGSITSGT LTSITSGSSS ATESGSSVSG SSSATESGSS VSGSTSATES 600
    GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSVSGSTSAT 650
    ESGSSASGSS SATESGSASS VPSSSGSVTE SGSSSSASES SITQSGTASG 700
    SSASSTSGSV TQSGSSVSGS SASSAPGISS SIPQSTSSAS TASGSITSGT 750
    LTSITSGSSS ATESGSSASG SSSATESGSS VSGSTSATES GSSVSGSTSA 800
    TESGSSASGS SSATESGSSV SGSTSATESG SSASGSSSAT ESGSASSVPS 850
    SSGSVTESGS SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS 900
    STSGSVTQSG SSVSGSSASS APGISSSIPQ STSSASTASG SITSGTLTSI 950
    TSSASSASAT ASNSLSSSDG TIYLPTTTIS GDLTLTGKVI ATEGVVVAAG 1000
    AKLTLLDGDK YSFSADLKVY GDLLVKKSKE TYPGTEFDIS GENFDVTGNF 1050
    NAEESAATSA SIYSFTPSSF DNSGDISLSL SKSKKGEVTF SPYSNSGAFS 1100
    FSNAILNGGS VSGLQRRDDT EGSVNNGEIN LDNGSTYVIV EPVSGKGTVN 1150
    IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTESNTTPIP VVGYTGENQI 1200
    AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF NVSEGTFAGA 1250
    YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG 1300
    ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT 1350
    TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN 1400
    GCNTKTVTSE APEATTTTVS PKTYTTATVT QCDDNGCSTK TVTSECPEET 1450
    SATTTSPKSY TTVTVTHCDD NGCNTKTVTS EAPEATTTTV SPKTYTTATV 1500
    TQCDDNGCST KTVTSECPEE TSATTTSPKS YTTVTVTHCD DNGCNTKTVT 1550
    SEAPEATTTT VSPKTYTTAT VTQCDDNGCS TKTVTSEAPK ETSETSETSA 1600
    APKTYTTATV TQCDDNGCNV KIITSQIPEA TSTVTATSAS PKSYTTVTSE 1650
    GSKATSLTTA ISKASSAIST YSKSAAPIKT STGIIVQSEG IAAGLNANTL 1700
    NALVGIFVLA FFN 1713
    Length:1,713
    Mass (Da):166,884
    Last modified:June 1, 2002 - v1
    Checksum:i9321A296EB586404
    GO

    Polymorphismi

    Half of the downstream region (residues 1035 to 1713) of the AWA1 gene was lost in the nonfoaming strain K701 due to a chromosomal recombination event.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1036 – 1713678Missing in strain: Kyokai no. 701, non-foaming mutant derivative of Kyokai no. 7.
    Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB071164 Genomic DNA. Translation: BAB85832.1.
    AB110100 Genomic DNA. Translation: BAD06576.1.
    AB110101 Genomic DNA. Translation: BAD06577.1.
    DG000051 Genomic DNA. Translation: GAA26176.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Of froth and haze - Issue 78 of January 2007

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB071164 Genomic DNA. Translation: BAB85832.1 .
    AB110100 Genomic DNA. Translation: BAD06576.1 .
    AB110101 Genomic DNA. Translation: BAD06577.1 .
    DG000051 Genomic DNA. Translation: GAA26176.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8TGE1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    SGDi S000029703. AWA1.

    Phylogenomic databases

    eggNOGi NOG12793.
    OrthoDBi EOG7VQJPN.

    Gene expression databases

    Genevestigatori Q8TGE1.

    Family and domain databases

    InterProi IPR000992. SRP1_TIP1.
    [Graphical view ]
    Pfami PF00660. SRP1_TIP1. 1 hit.
    [Graphical view ]
    PROSITEi PS00724. SRP1_TIP1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast."
      Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K.
      Appl. Environ. Microbiol. 68:2018-2025(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, BIOTECHNOLOGY.
      Strain: Kyokai no. 7 / NBRC 101557.
    2. "Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake Yeast."
      Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H.
      J. Biosci. Bioeng. 97:14-18(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Kyokai no. 7 / NBRC 101557 and Kyokai no. 701.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Kyokai no. 7 / NBRC 101557.

    Entry informationi

    Entry nameiAWA1_YEASK
    AccessioniPrimary (citable) accession number: Q8TGE1
    Secondary accession number(s): G2WM83, Q76C74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    'Awa' means 'foam' in Japanese.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3