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Q8TG90

- RIB3_MAGO7

UniProt

Q8TG90 - RIB3_MAGO7

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Protein

3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene

RIB3

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.By similarity

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Magnesium or manganese.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi37 – 371Magnesium or manganese 1
Metal bindingi37 – 371Magnesium or manganese 2
Binding sitei41 – 411SubstrateBy similarity
Sitei136 – 1361Essential for catalytic activityBy similarity
Metal bindingi153 – 1531Magnesium or manganese 2
Binding sitei174 – 1741SubstrateBy similarity
Sitei174 – 1741Essential for catalytic activityBy similarity

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Riboflavin biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00275; UER00399.

Names & Taxonomyi

Protein namesi
Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase (EC:4.1.99.12)
Short name:
DHBP synthase
Gene namesi
Name:RIB3
ORF Names:MGG_01049
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
ProteomesiUP000009058: Chromosome 5

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2332333,4-dihydroxy-2-butanone 4-phosphate synthasePRO_0000296686Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi148305.Q8TG90.

Structurei

Secondary structure

1
233
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 239
Beta strandi28 – 314
Turni34 – 374
Beta strandi40 – 456
Helixi46 – 483
Helixi51 – 6010
Beta strandi66 – 694
Helixi71 – 766
Beta strandi98 – 1003
Beta strandi106 – 1083
Helixi111 – 12212
Helixi128 – 1303
Beta strandi131 – 14111
Helixi146 – 1494
Helixi153 – 16311
Beta strandi168 – 17710
Beta strandi180 – 1823
Beta strandi188 – 1903
Helixi196 – 20510
Beta strandi209 – 2124
Helixi213 – 22412

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K49X-ray1.50A1-233[»]
1K4IX-ray0.98A1-233[»]
1K4LX-ray1.60A1-233[»]
1K4OX-ray1.10A1-233[»]
1K4PX-ray1.00A1-233[»]
ProteinModelPortaliQ8TG90.
SMRiQ8TG90. Positions 12-227.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TG90.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 372Substrate bindingBy similarity
Regioni150 – 1545Substrate binding

Sequence similaritiesi

Belongs to the DHBP synthase family.Curated

Phylogenomic databases

eggNOGiCOG0108.
InParanoidiQ8TG90.
KOiK02858.
OrthoDBiEOG76TB2T.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TG90-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSTDSIPKS NFDAIPDVIQ AFKNGEFVVV LDDPSRENEA DLIIAAESVT
60 70 80 90 100
TEQMAFMVRH SSGLICAPLT PERTTALDLP QMVTHNADPR GTAYTVSVDA
110 120 130 140 150
EHPSTTTGIS AHDRALACRM LAAPDAQPSH FRRPGHVFPL RAVAGGVRAR
160 170 180 190 200
RGHTEAGVEL CRLAGKRPVA VISEIVDDGQ EVEGRAVRAA PGMLRGDECV
210 220 230
AFARRWGLKV CTIEDMIAHV EKTEGKLETN GSG
Length:233
Mass (Da):25,003
Last modified:June 1, 2002 - v1
Checksum:i5C83AA5C49DFD07E
GO

Mass spectrometryi

Molecular mass is 24870.7 Da from positions 2 - 233. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF430214 mRNA. Translation: AAL84175.1.
CM001235 Genomic DNA. Translation: EHA48306.1.
RefSeqiXP_003717890.1. XM_003717842.1.

Genome annotation databases

EnsemblFungiiMGG_01049T0; MGG_01049T0; MGG_01049.
GeneIDi2674307.
KEGGimgr:MGG_01049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF430214 mRNA. Translation: AAL84175.1 .
CM001235 Genomic DNA. Translation: EHA48306.1 .
RefSeqi XP_003717890.1. XM_003717842.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K49 X-ray 1.50 A 1-233 [» ]
1K4I X-ray 0.98 A 1-233 [» ]
1K4L X-ray 1.60 A 1-233 [» ]
1K4O X-ray 1.10 A 1-233 [» ]
1K4P X-ray 1.00 A 1-233 [» ]
ProteinModelPortali Q8TG90.
SMRi Q8TG90. Positions 12-227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 148305.Q8TG90.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii MGG_01049T0 ; MGG_01049T0 ; MGG_01049 .
GeneIDi 2674307.
KEGGi mgr:MGG_01049.

Phylogenomic databases

eggNOGi COG0108.
InParanoidi Q8TG90.
KOi K02858.
OrthoDBi EOG76TB2T.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .

Miscellaneous databases

EvolutionaryTracei Q8TG90.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea."
    Liao D.-I., Viitanen P.V., Jordan D.B.
    Acta Crystallogr. D 56:1495-1497(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17, CHARACTERIZATION, COFACTOR, MASS SPECTROMETRY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
  3. "Structural definition of the active site and catalytic mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase."
    Liao D.-I., Zheng Y.-J., Viitanen P.V., Jordan D.B.
    Biochemistry 41:1795-1806(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.98 ANGSTROMS) IN COMPLEXES WITH DIVALENT METAL CATIONS AND SUBSTRATE ANALOGS, SUBUNIT.

Entry informationi

Entry nameiRIB3_MAGO7
AccessioniPrimary (citable) accession number: Q8TG90
Secondary accession number(s): G4NCK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3