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Q8TG90

- RIB3_MAGO7

UniProt

Q8TG90 - RIB3_MAGO7

Protein

3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene

RIB3

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.By similarity

    Catalytic activityi

    D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Magnesium or manganese.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi37 – 371Magnesium or manganese 1
    Metal bindingi37 – 371Magnesium or manganese 2
    Binding sitei41 – 411SubstrateBy similarity
    Sitei136 – 1361Essential for catalytic activityBy similarity
    Metal bindingi153 – 1531Magnesium or manganese 2
    Binding sitei174 – 1741SubstrateBy similarity
    Sitei174 – 1741Essential for catalytic activityBy similarity

    GO - Molecular functioni

    1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. riboflavin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Riboflavin biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00275; UER00399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3,4-dihydroxy-2-butanone 4-phosphate synthase (EC:4.1.99.12)
    Short name:
    DHBP synthase
    Gene namesi
    Name:RIB3
    ORF Names:MGG_01049
    OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
    Taxonomic identifieri242507 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
    ProteomesiUP000009058: Chromosome 5

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2332333,4-dihydroxy-2-butanone 4-phosphate synthasePRO_0000296686Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi148305.Q8TG90.

    Structurei

    Secondary structure

    1
    233
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 239
    Beta strandi28 – 314
    Turni34 – 374
    Beta strandi40 – 456
    Helixi46 – 483
    Helixi51 – 6010
    Beta strandi66 – 694
    Helixi71 – 766
    Beta strandi98 – 1003
    Beta strandi106 – 1083
    Helixi111 – 12212
    Helixi128 – 1303
    Beta strandi131 – 14111
    Helixi146 – 1494
    Helixi153 – 16311
    Beta strandi168 – 17710
    Beta strandi180 – 1823
    Beta strandi188 – 1903
    Helixi196 – 20510
    Beta strandi209 – 2124
    Helixi213 – 22412

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K49X-ray1.50A1-233[»]
    1K4IX-ray0.98A1-233[»]
    1K4LX-ray1.60A1-233[»]
    1K4OX-ray1.10A1-233[»]
    1K4PX-ray1.00A1-233[»]
    ProteinModelPortaliQ8TG90.
    SMRiQ8TG90. Positions 12-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8TG90.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni36 – 372Substrate bindingBy similarity
    Regioni150 – 1545Substrate binding

    Sequence similaritiesi

    Belongs to the DHBP synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG0108.
    KOiK02858.
    OrthoDBiEOG76TB2T.

    Family and domain databases

    Gene3Di3.90.870.10. 1 hit.
    InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
    IPR000422. DHBP_synthase_RibB.
    [Graphical view]
    PfamiPF00926. DHBP_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55821. SSF55821. 1 hit.
    TIGRFAMsiTIGR00506. ribB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8TG90-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSTDSIPKS NFDAIPDVIQ AFKNGEFVVV LDDPSRENEA DLIIAAESVT    50
    TEQMAFMVRH SSGLICAPLT PERTTALDLP QMVTHNADPR GTAYTVSVDA 100
    EHPSTTTGIS AHDRALACRM LAAPDAQPSH FRRPGHVFPL RAVAGGVRAR 150
    RGHTEAGVEL CRLAGKRPVA VISEIVDDGQ EVEGRAVRAA PGMLRGDECV 200
    AFARRWGLKV CTIEDMIAHV EKTEGKLETN GSG 233
    Length:233
    Mass (Da):25,003
    Last modified:June 1, 2002 - v1
    Checksum:i5C83AA5C49DFD07E
    GO

    Mass spectrometryi

    Molecular mass is 24870.7 Da from positions 2 - 233. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF430214 mRNA. Translation: AAL84175.1.
    CM001235 Genomic DNA. Translation: EHA48306.1.
    RefSeqiXP_003717890.1. XM_003717842.1.

    Genome annotation databases

    EnsemblFungiiMGG_01049T0; MGG_01049T0; MGG_01049.
    GeneIDi2674307.
    KEGGimgr:MGG_01049.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF430214 mRNA. Translation: AAL84175.1 .
    CM001235 Genomic DNA. Translation: EHA48306.1 .
    RefSeqi XP_003717890.1. XM_003717842.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K49 X-ray 1.50 A 1-233 [» ]
    1K4I X-ray 0.98 A 1-233 [» ]
    1K4L X-ray 1.60 A 1-233 [» ]
    1K4O X-ray 1.10 A 1-233 [» ]
    1K4P X-ray 1.00 A 1-233 [» ]
    ProteinModelPortali Q8TG90.
    SMRi Q8TG90. Positions 12-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 148305.Q8TG90.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii MGG_01049T0 ; MGG_01049T0 ; MGG_01049 .
    GeneIDi 2674307.
    KEGGi mgr:MGG_01049.

    Phylogenomic databases

    eggNOGi COG0108.
    KOi K02858.
    OrthoDBi EOG76TB2T.

    Enzyme and pathway databases

    UniPathwayi UPA00275 ; UER00399 .

    Miscellaneous databases

    EvolutionaryTracei Q8TG90.

    Family and domain databases

    Gene3Di 3.90.870.10. 1 hit.
    InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
    IPR000422. DHBP_synthase_RibB.
    [Graphical view ]
    Pfami PF00926. DHBP_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55821. SSF55821. 1 hit.
    TIGRFAMsi TIGR00506. ribB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea."
      Liao D.-I., Viitanen P.V., Jordan D.B.
      Acta Crystallogr. D 56:1495-1497(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17, CHARACTERIZATION, COFACTOR, MASS SPECTROMETRY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
    3. "Structural definition of the active site and catalytic mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase."
      Liao D.-I., Zheng Y.-J., Viitanen P.V., Jordan D.B.
      Biochemistry 41:1795-1806(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (0.98 ANGSTROMS) IN COMPLEXES WITH DIVALENT METAL CATIONS AND SUBSTRATE ANALOGS, SUBUNIT.

    Entry informationi

    Entry nameiRIB3_MAGO7
    AccessioniPrimary (citable) accession number: Q8TG90
    Secondary accession number(s): G4NCK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3