3,4-dihydroxy-2-butanone 4-phosphate synthase - Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus)

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Q8TG90 (RIB3_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12

Gene names

Name:	RIB3
ORF Names:	MGG_01049

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe ›

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese. Ref.1
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.
Subunit structure	Homodimer. Ref.3
Sequence similarities	Belongs to the DHBP synthase family.
Mass spectrometry	Molecular mass is 24870.7 Da from positions 2 - 233. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 233

233

3,4-dihydroxy-2-butanone 4-phosphate synthase

PRO_0000296686

Regions

Region

36 – 37

Substrate binding By similarity

Region

150 – 154

Substrate binding

Sites

Metal binding

Magnesium or manganese 1

Metal binding

Magnesium or manganese 2

Metal binding

153

Magnesium or manganese 2

Binding site

Substrate By similarity

Binding site

174

Substrate By similarity

Site

136

Essential for catalytic activity By similarity

Site

174

Essential for catalytic activity By similarity

Secondary structure

233

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q8TG90 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 5C83AA5C49DFD07E
Show »

FASTA

233

25,003

        10         20         30         40         50         60 
MPSTDSIPKS NFDAIPDVIQ AFKNGEFVVV LDDPSRENEA DLIIAAESVT TEQMAFMVRH 

        70         80         90        100        110        120 
SSGLICAPLT PERTTALDLP QMVTHNADPR GTAYTVSVDA EHPSTTTGIS AHDRALACRM 

       130        140        150        160        170        180 
LAAPDAQPSH FRRPGHVFPL RAVAGGVRAR RGHTEAGVEL CRLAGKRPVA VISEIVDDGQ 

       190        200        210        220        230 
EVEGRAVRAA PGMLRGDECV AFARRWGLKV CTIEDMIAHV EKTEGKLETN GSG

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea." Liao D.-I., Viitanen P.V., Jordan D.B. Acta Crystallogr. D 56:1495-1497(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17, CHARACTERIZATION, COFACTOR, MASS SPECTROMETRY.
[2]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. Birren B.W. Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
[3]	"Structural definition of the active site and catalytic mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase." Liao D.-I., Zheng Y.-J., Viitanen P.V., Jordan D.B. Biochemistry 41:1795-1806(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (0.98 ANGSTROMS) IN COMPLEXES WITH DIVALENT METAL CATIONS AND SUBSTRATE ANALOGS, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF430214 mRNA. Translation: AAL84175.1.
CM001235 Genomic DNA. Translation: EHA48306.1.

RefSeq

XP_003717890.1. XM_003717842.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K49	X-ray	1.50	A	1-233	[»]
1K4I	X-ray	0.98	A	1-233	[»]
1K4L	X-ray	1.60	A	1-233	[»]
1K4O	X-ray	1.10	A	1-233	[»]
1K4P	X-ray	1.00	A	1-233	[»]

ProteinModelPortal

Q8TG90.

SMR

Q8TG90. Positions 12-227.

ModBase

Search...

Protein-protein interaction databases

STRING

148305.Q8TG90.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

MGG_01049T0; MGG_01049T0; MGG_01049.

GeneID

2674307.

KEGG

mgr:MGG_01049.

Phylogenomic databases

eggNOG

COG0108.

K02858.

OrthoDB

EOG4NCQP1.

Enzyme and pathway databases

UniPathway

UPA00275; UER00399.

Family and domain databases

Gene3D

3.90.870.10. 1 hit.

InterPro

IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]

Pfam

PF00926. DHBP_synthase. 1 hit.
[Graphical view]

SUPFAM

SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.

TIGRFAMs

TIGR00506. ribB. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q8TG90.

Entry information

Entry name

RIB3_MAGO7

Accession

Primary (citable) accession number: Q8TG90
Secondary accession number(s): G4NCK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents