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Protein

3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene

RIB3

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.By similarity

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.1 Publication

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase (RIB3)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Magnesium or manganese 11
Metal bindingi37Magnesium or manganese 21
Binding sitei41SubstrateBy similarity1
Sitei136Essential for catalytic activityBy similarity1
Metal bindingi153Magnesium or manganese 21
Binding sitei174SubstrateBy similarity1
Sitei174Essential for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Riboflavin biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00275; UER00399.

Names & Taxonomyi

Protein namesi
Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase (EC:4.1.99.12)
Short name:
DHBP synthase
Gene namesi
Name:RIB3
ORF Names:MGG_01049
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiFungiDB:MGG_01049.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002966861 – 2333,4-dihydroxy-2-butanone 4-phosphate synthaseAdd BLAST233

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 23Combined sources9
Beta strandi28 – 31Combined sources4
Turni34 – 37Combined sources4
Beta strandi40 – 45Combined sources6
Helixi46 – 48Combined sources3
Helixi51 – 60Combined sources10
Beta strandi66 – 69Combined sources4
Helixi71 – 76Combined sources6
Beta strandi98 – 100Combined sources3
Beta strandi106 – 108Combined sources3
Helixi111 – 122Combined sources12
Helixi128 – 130Combined sources3
Beta strandi131 – 141Combined sources11
Helixi146 – 149Combined sources4
Helixi153 – 163Combined sources11
Beta strandi168 – 177Combined sources10
Beta strandi180 – 182Combined sources3
Beta strandi188 – 190Combined sources3
Helixi196 – 205Combined sources10
Beta strandi209 – 212Combined sources4
Helixi213 – 224Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K49X-ray1.50A1-233[»]
1K4IX-ray0.98A1-233[»]
1K4LX-ray1.60A1-233[»]
1K4OX-ray1.10A1-233[»]
1K4PX-ray1.00A1-233[»]
ProteinModelPortaliQ8TG90.
SMRiQ8TG90.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TG90.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 37Substrate bindingBy similarity2
Regioni150 – 154Substrate binding5

Sequence similaritiesi

Belongs to the DHBP synthase family.Curated

Phylogenomic databases

InParanoidiQ8TG90.
KOiK02858.
OrthoDBiEOG092C42BA.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TG90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTDSIPKS NFDAIPDVIQ AFKNGEFVVV LDDPSRENEA DLIIAAESVT
60 70 80 90 100
TEQMAFMVRH SSGLICAPLT PERTTALDLP QMVTHNADPR GTAYTVSVDA
110 120 130 140 150
EHPSTTTGIS AHDRALACRM LAAPDAQPSH FRRPGHVFPL RAVAGGVRAR
160 170 180 190 200
RGHTEAGVEL CRLAGKRPVA VISEIVDDGQ EVEGRAVRAA PGMLRGDECV
210 220 230
AFARRWGLKV CTIEDMIAHV EKTEGKLETN GSG
Length:233
Mass (Da):25,003
Last modified:June 1, 2002 - v1
Checksum:i5C83AA5C49DFD07E
GO

Mass spectrometryi

Molecular mass is 24870.7 Da from positions 2 - 233. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF430214 mRNA. Translation: AAL84175.1.
CM001235 Genomic DNA. Translation: EHA48306.1.
RefSeqiXP_003717890.1. XM_003717842.1.

Genome annotation databases

EnsemblFungiiMGG_01049T0; MGG_01049T0; MGG_01049.
GeneIDi2674307.
KEGGimgr:MGG_01049.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF430214 mRNA. Translation: AAL84175.1.
CM001235 Genomic DNA. Translation: EHA48306.1.
RefSeqiXP_003717890.1. XM_003717842.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K49X-ray1.50A1-233[»]
1K4IX-ray0.98A1-233[»]
1K4LX-ray1.60A1-233[»]
1K4OX-ray1.10A1-233[»]
1K4PX-ray1.00A1-233[»]
ProteinModelPortaliQ8TG90.
SMRiQ8TG90.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_01049T0; MGG_01049T0; MGG_01049.
GeneIDi2674307.
KEGGimgr:MGG_01049.

Organism-specific databases

EuPathDBiFungiDB:MGG_01049.

Phylogenomic databases

InParanoidiQ8TG90.
KOiK02858.
OrthoDBiEOG092C42BA.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00399.

Miscellaneous databases

EvolutionaryTraceiQ8TG90.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00506. ribB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIB3_MAGO7
AccessioniPrimary (citable) accession number: Q8TG90
Secondary accession number(s): G4NCK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2002
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.