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Q8TG90 (RIB3_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase

Short name=DHBP synthase
EC=4.1.99.12
Gene names
Name:RIB3
ORF Names:MGG_01049
OrganismMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]
Taxonomic identifier242507 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese. Ref.1

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.

Subunit structure

Homodimer. Ref.3

Sequence similarities

Belongs to the DHBP synthase family.

Mass spectrometry

Molecular mass is 24870.7 Da from positions 2 - 233. Determined by ESI. Ref.1

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2332333,4-dihydroxy-2-butanone 4-phosphate synthase
PRO_0000296686

Regions

Region36 – 372Substrate binding By similarity
Region150 – 1545Substrate binding

Sites

Metal binding371Magnesium or manganese 1
Metal binding371Magnesium or manganese 2
Metal binding1531Magnesium or manganese 2
Binding site411Substrate By similarity
Binding site1741Substrate By similarity
Site1361Essential for catalytic activity By similarity
Site1741Essential for catalytic activity By similarity

Secondary structure

........................................ 233
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8TG90 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 5C83AA5C49DFD07E

FASTA23325,003
        10         20         30         40         50         60 
MPSTDSIPKS NFDAIPDVIQ AFKNGEFVVV LDDPSRENEA DLIIAAESVT TEQMAFMVRH 

        70         80         90        100        110        120 
SSGLICAPLT PERTTALDLP QMVTHNADPR GTAYTVSVDA EHPSTTTGIS AHDRALACRM 

       130        140        150        160        170        180 
LAAPDAQPSH FRRPGHVFPL RAVAGGVRAR RGHTEAGVEL CRLAGKRPVA VISEIVDDGQ 

       190        200        210        220        230 
EVEGRAVRAA PGMLRGDECV AFARRWGLKV CTIEDMIAHV EKTEGKLETN GSG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, purification and crystallization of dihydroxybutanone phosphate synthase from Magnaporthe grisea."
Liao D.-I., Viitanen P.V., Jordan D.B.
Acta Crystallogr. D 56:1495-1497(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17, CHARACTERIZATION, COFACTOR, MASS SPECTROMETRY.
[2]"The genome sequence of the rice blast fungus Magnaporthe grisea."
Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. expand/collapse author list , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.
Nature 434:980-986(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
[3]"Structural definition of the active site and catalytic mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase."
Liao D.-I., Zheng Y.-J., Viitanen P.V., Jordan D.B.
Biochemistry 41:1795-1806(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (0.98 ANGSTROMS) IN COMPLEXES WITH DIVALENT METAL CATIONS AND SUBSTRATE ANALOGS, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF430214 mRNA. Translation: AAL84175.1.
CM001235 Genomic DNA. Translation: EHA48306.1.
RefSeqXP_003717890.1. XM_003717842.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K49X-ray1.50A1-233[»]
1K4IX-ray0.98A1-233[»]
1K4LX-ray1.60A1-233[»]
1K4OX-ray1.10A1-233[»]
1K4PX-ray1.00A1-233[»]
ProteinModelPortalQ8TG90.
SMRQ8TG90. Positions 12-227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING148305.Q8TG90.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiMGG_01049T0; MGG_01049T0; MGG_01049.
GeneID2674307.
KEGGmgr:MGG_01049.

Phylogenomic databases

eggNOGCOG0108.
KOK02858.
OrthoDBEOG76TB2T.

Enzyme and pathway databases

UniPathwayUPA00275; UER00399.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
[Graphical view]
SUPFAMSSF55821. SSF55821. 1 hit.
TIGRFAMsTIGR00506. ribB. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ8TG90.

Entry information

Entry nameRIB3_MAGO7
AccessionPrimary (citable) accession number: Q8TG90
Secondary accession number(s): G4NCK9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2002
Last modified: November 13, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways