Reviewed, UniProtKB/Swiss-Prot Q8TFN2 (ATG1_PICAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATG1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1
    Peroxisome degradation deficient protein 7

Gene names

Name:	ATG1
Synonyms:	PDD7

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in glucose-induced peroxisome degradation (micropexophagy), ethanol-induced macropexophagy and microautophagy. Probably also involved in cytoplasm to vacuole transport (Cvt). Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 804	804	Serine/threonine-protein kinase ATG1		PRO_0000085650
Regions
	Domain	12 – 308	297	Protein kinase
	Nucleotide binding	18 – 26	9	ATP By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	41	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TFN2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 052186AA1F7FC585	FASTA	804	89,844
	10 20 30 40 50 60 MSKHQTQVVG DFTIGPEIGR GSFANVYKGY DNRTKAPVAV KSVFRSRLKN QKLVENLEIE 70 80 90 100 110 120 ISILKNLKNP HIVALLDCVK TDQYFHLFME YCSLGDLSYF IRRRDQLVQT HPLISSILER 130 140 150 160 170 180 YPSPPNSHGL NKVLVVNFLK QLASALEFLR DQNLVHRDIK PQNLLLSPPV HSKEEFKRKG 190 200 210 220 230 240 YSGLWELPVL KIADFGFARF LPSTSMAETL CGSPLYMAPE ILRYEKYNAK ADLWSVGAVI 250 260 270 280 290 300 YEMSVGKPPF RASNHVELLR KIEKSKDEIT FPVSAEVPDD LVRLICGLLK ANPTERMGFQ 310 320 330 340 350 360 EFFNDPLIVY DVQCADEPLE CSNVDEQLFI SEYLPNLKTS PPAKPAPETI KEESEEEERA 370 380 390 400 410 420 ERAPTDSLLI GKEADLRIPR PMEGSGKDEV IKKLINKSSP PPDTVKDGQI KKGARRDKDD 430 440 450 460 470 480 FVYEKDYVVV EKRTVEVNAI ADELAKAGAG AVAIPSPHLG TNEHSAANPS GPTETQTQRR 490 500 510 520 530 540 FSPSSRTSSI GSNRRPSWGD RKMPISISPT NALTKALGYT SNRLFGQQQQ QPQQAQQAAI 550 560 570 580 590 600 ESAITNVTTN LLATKTLRPL KPSQETSLED TEVINQLELL ATMAHAISLF AEVKFSQLIP 610 620 630 640 650 660 LPPSSSSPGS ADYDEMYQND AFPPQMVKSI SSEGVALYVE TLSLLAKAMS IASDWWHQNS 670 680 690 700 710 720 SKPSTSPKLN DLVQWIRSRF NESLEKAEFL RLRLADANEQ LVGESGSSLN KPVVAEKLIF 730 740 750 760 770 780 DRALEMSRTA AMNELKNEDL LGCELSYSTA IWMLEALLSN DEEPVTGNEK LDAEDKKIIE 790 800 LFINSIGNRL KVLRQKIDKQ GVRS

References

[1]	"The Hansenula polymorpha PDD7 gene is essential for micropexophagy and microautophagy." Komduur J.A., Veenhuis M., Kiel J.A.K.W. FEMS Yeast Res. 3:27-34(2003) [PubMed: 12702243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / IFO 1476 / NRRL Y-5445.
[2]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY053423 Genomic DNA. Translation: AAL23618.1.
3D structure databases
HSSP	HSSP built from PDB template 1NY3 based on UniProtKB P49137.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.1. 276392.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_PICAN

Accession

Primary (citable) accession number: Q8TFN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)