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Q8TFH7

- SEN2_SCHPO

UniProt

Q8TFH7 - SEN2_SCHPO

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Protein

Probable tRNA-splicing endonuclease subunit sen2

Gene

sen2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei281 – 2811By similarity
Active sitei289 – 2891By similarity
Active sitei325 – 3251By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: PomBase

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA-type intron splice site recognition and cleavage Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen2
Gene namesi
Name:sen2
ORF Names:SPAPB17E12.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAPB17E12.07c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. mitochondrial outer membrane Source: PomBase
  3. tRNA-intron endonuclease complex Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Probable tRNA-splicing endonuclease subunit sen2PRO_0000109461Add
BLAST

Proteomic databases

MaxQBiQ8TFH7.

Interactioni

Subunit structurei

Heterotetramer composed of sen2, sen15, sen34 and sen54. Interacts directly with sen54 (By similarity).By similarity

Protein-protein interaction databases

STRINGi4896.SPAPB17E12.07c-1.

Structurei

3D structure databases

ProteinModelPortaliQ8TFH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000159660.
InParanoidiQ8TFH7.
KOiK15322.
OMAiSPACLAH.
OrthoDBiEOG7SXWFH.
PhylomeDBiQ8TFH7.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8TFH7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKNHEVYKD ALPISLAYPL PPIILTNPLT WIPYIYRYLF RKTPRQVQWQ
60 70 80 90 100
CQLHESDLSC VVTDSEAIKK FWTSGFFGKG NLSRSEPTWH TRTKRSLGLL
110 120 130 140 150
GFDEDLVAEE VTARRRFQRK QFKAQRAYRE NRARERQLLL ENGKPIPASL
160 170 180 190 200
EEDAELPEYL TKSLKDFSRV SENPYHITSV PNVEHLQLTF PEAFFLASLG
210 220 230 240 250
VLRINYENPN FELLPILKLF ANIVANSVAL THDYSLQQSH EDPIIEPDNK
260 270 280 290 300
FLTELAAYFY FRQQGWVVKN GTKFSVDFLL YKKGPVFSHA EFAILLIPCV
310 320 330 340 350
GNKQKYNMQW HEVHCLNRVI AQVKKSLILC YVQCPSIEDF NKIWKNQASM
360 370 380
NEWDWAESVL RQYLIRCVTL RRWVPSRNRD
Length:380
Mass (Da):44,608
Last modified:June 1, 2002 - v1
Checksum:iE458D08F0D920249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD27500.1.
RefSeqiNP_001018222.1. NM_001018702.2.

Genome annotation databases

EnsemblFungiiSPAPB17E12.07c.1; SPAPB17E12.07c.1:pep; SPAPB17E12.07c.
GeneIDi3361398.
KEGGispo:SPAPB17E12.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAD27500.1 .
RefSeqi NP_001018222.1. NM_001018702.2.

3D structure databases

ProteinModelPortali Q8TFH7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4896.SPAPB17E12.07c-1.

Proteomic databases

MaxQBi Q8TFH7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAPB17E12.07c.1 ; SPAPB17E12.07c.1:pep ; SPAPB17E12.07c .
GeneIDi 3361398.
KEGGi spo:SPAPB17E12.07c.

Organism-specific databases

PomBasei SPAPB17E12.07c.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000159660.
InParanoidi Q8TFH7.
KOi K15322.
OMAi SPACLAH.
OrthoDBi EOG7SXWFH.
PhylomeDBi Q8TFH7.

Miscellaneous databases

NextBioi 20811448.
PROi Q8TFH7.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR016589. tRNA_splic_SEN2.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiSEN2_SCHPO
AccessioniPrimary (citable) accession number: Q8TFH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3