Q8TFG7 (TRM5_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(37)-N1)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5

Gene names

Name:	trm5
ORF Names:	SPAPB18E9.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion matrix By similarity. Nucleus. Cytoplasm By similarity. Note: Predominantly in the mitochondria and in the nucleus By similarity. Ref.2
Sequence similarities	Belongs to the TRM5 / TYW2 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Mitochondrion Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	tRNA (guanine) methyltransferase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	tRNA (guanine(37)-N1)-methyltransferase		PRO_0000316612
Regions
	Region	269 – 270	2	S-adenosyl-L-methionine binding By similarity
	Region	297 – 298	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	231	1	S-adenosyl-L-methionine By similarity
	Binding site	354	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TFG7 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 370B805E7445F603	FASTA	450	51,748
	10 20 30 40 50 60 MHLQIRTVHN MAELLHPNKV EMKPFLKNLD KNLFKKTYNL VAAKVSPKKV GLLNKVCKKD 70 80 90 100 110 120 LLDWPRVKHV YQQNNEKLVL LNRDASLDGT RGLSDATVSF IKENNIELVP FQLTLDYDYW 130 140 150 160 170 180 RADDILDAIL PPGEKEDHPS GFTAVGHIAH MNLREEWLPY KYIIGKVILD KNPSIETVVN 190 200 210 220 230 240 KTDTIDTKFR TFQMEVLAGK DDFIVTQSES NCKFRFDFSK VYWNSRLSTE HDRLIQQFQP 250 260 270 280 290 300 GDAVCDVMAG VGPFACPAGK KNVIVFANDL NPYSYESLVE NIFLNKVANF VKAFNQDGRE 310 320 330 340 350 360 FIRSSVQKLL GFSKDEKAIT VFPPRKRARK LEENKDPVRQ DIPIPPVFSH YVMNLPGSAI 370 380 390 400 410 420 EFLDAFKGCY YGLEYLFKDR SLPKVHVHCF CRFPDPEEDL INRIYASLGY RFSPEEVDFY 430 440 450 YVRKVAPNKD MYCCTFTLPG SIIFAKPVSG

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAD27467.2.
RefSeq	NP_001018269.2. NM_001019824.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8TFG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAPB18E9.01.1; SPAPB18E9.01.1:pep; SPAPB18E9.01.
GeneID	3361429.
GenomeReviews	Gene locus trm5 in contig CU329670_GR.
KEGG	spo:SPAPB18E9.01.
Organism-specific databases
GeneDB_Spombe	SPAPB18E9.01.
Phylogenomic databases
eggNOG	fuNOG04366.
GeneTree	EFGT00050000006070.
HOGENOM	HBG592917.
OMA	YVMNLPD.
OrthoDB	EOG4PVS7B.
Gene expression databases
ArrayExpress	Q8TFG7.
Family and domain databases
InterPro	IPR003402. tRNA_Trfase_Trm5/Tyw2. [Graphical view]
KO	K15429.
Pfam	PF02475. Met_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM5_SCHPO

Accession

Primary (citable) accession number: Q8TFG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 22, 2009
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program