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Q8TFF8 (ASPG4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable L-asparaginase 4
EC=3.5.1.1
Alternative name(s):
L-asparagine amidohydrolase 4
Gene names
ORF Names:SPBPB8B6.05c, SPAP8B6.05c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-asparagine + H2O = L-aspartate + NH3.

Subcellular location

Secreted › cell wall By similarity.

Sequence similarities

Belongs to the asparaginase 1 family.

Ontologies

Keywords
   Cellular componentCell wall
Secreted
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processasparagine metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcell surface

Non-traceable author statement. Source: GeneDB_Spombe

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from direct assay. Source: GeneDB_Spombe

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionasparaginase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 356334Probable L-asparaginase 4
PRO_0000002366

Regions

Region126 – 1272Substrate binding By similarity

Sites

Active site461O-isoaspartyl threonine intermediate By similarity
Binding site931Substrate By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q8TFF8 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: E7935A592DB06FDA

FASTA35638,057
        10         20         30         40         50         60 
MWGFIVTCGI FLVLLCQLRL LSKRKSKSTP YNALLPNVTV FAMGGTIAGC ANSSLEIVNY 

        70         80         90        100        110        120 
IPGSVGIEKL IEAVPAIKAI ANINGVQVTN MGSENLTPAD VLKLAKLILA EVAKPNVHGI 

       130        140        150        160        170        180 
VITHGTDSLE ETAMFLDLTI STAKPIVVVG AMRPSTAIGA DGPMNLLNAV AVASSNQSMG 

       190        200        210        220        230        240 
RGTLVLLNDR IGSAFYTTKT NGNTLDTFKS YEAGSLGIVL NQKPFYFFSP AVPTGKVFFD 

       250        260        270        280        290        300 
IYNIKQLPRV DILYGYQGLN PKLAESAVHL GAKGLVLAAM GATSWTDDGN EVISSLIREH 

       310        320        330        340        350 
NIPVVYSHRT AEGYSSNSCL GIPSYFLNPQ KARYMLMLAI SSGYSIRDIE GLFSIK

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAD27911.1.
RefSeqNP_001018766.1. NM_001020941.1.

3D structure databases

ProteinModelPortalQ8TFF8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8TFF8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBPB8B6.05c.1; SPBPB8B6.05c.1:pep; SPBPB8B6.05c.
GeneID3361187.
KEGGspo:SPBPB8B6.05c.

Organism-specific databases

GeneDB_SpombeSPBPB8B6.05c.

Phylogenomic databases

eggNOGfuNOG07200.
GeneTreeEFGT00050000004559.
HOGENOMHBG497495.
OrthoDBEOG4P5PJT.

Family and domain databases

InterProIPR004550. AsnASE_II.
IPR006034. Asparaginase/glutaminase.
[Graphical view]
KOK01424.
PANTHERPTHR11707. Asp/Glutamnse. 1 hit.
PfamPF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSPR00139. ASNGLNASE.
SMARTSM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMSSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMsTIGR00520. AsnASE_II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameASPG4_SCHPO
AccessionPrimary (citable) accession number: Q8TFF8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2002
Last modified: December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

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