Reviewed, UniProtKB/Swiss-Prot Q8TFF6 (DHE4_SACBA)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase 1
      Short name=NADP-GDH 1
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase 1

Gene names

Name:

GDH1

Organism

Saccharomyces bayanus (Yeast) (Saccharomyces uvarum)

Taxonomic identifier

4931 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	NADP-specific glutamate dehydrogenase 1		PRO_0000182797
Regions
	Nucleotide binding	174 – 203	30	NAD By similarity
Sites
	Active site	110	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8TFF6-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 98DF52A68BDE0D12	FASTA	454	49,425
	10 20 30 40 50 60 MSEPEFQQAY DEVVSSLEDS TLFEQHPKYR KVLPIVSVPE RIIQFRVTWE NDKGEQEVAQ 70 80 90 100 110 120 GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS LTGLDMGGGK GGLCVDLKGR 130 140 150 160 170 180 SNNEIRRICY AFMRELSRHI GQDTDVPAGD IGVGGREIGY LFGAYRTYKN SWEGVLTGKG 190 200 210 220 230 240 LNWGGSLIRP EATGYGLVYY TQAMIDYATN GKESFEGKRV TISGSGNVAQ FAALKVIELG 250 260 270 280 290 300 GTVVSLSDSK GCIISETGIT SEQVADISSA KVNFKSLEQI VGEYSTFTEN KVQYISGARP 310 320 330 340 350 360 WTHVQKVDIA LPCATQNEVS GDEAKALVAQ GVKFVAEGSN MGSTPEAIAV FETARATAST 370 380 390 400 410 420 LKESVWYGPP KAANLGGVAV SGLEMAQNSQ RITWSSERVD QELKKIMVNC FNECIDSAKK 430 440 450 YTKEGNALPS LVKGANIASF IKVSDAMFDQ GDVF

References

[1]

"New S. pastorianus strains and Saccharomyces natural hybrids revealed by polyphasis identification of CBS strains formerly uncompletely identified by conventional method." Nguyen H.V. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76518 / CBS 7001 / NCYC 623 / NRRL Y-11845.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ418037 Genomic DNA. Translation: CAD10750.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 164752.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_SACBA

Accession

Primary (citable) accession number: Q8TFF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)