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Protein

Serine/threonine-protein kinase haspin

Gene

GSG2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Enzyme regulationi

Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei511ATP1
Active sitei649Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi490 – 498ATPPROSITE-ProRule annotationBy similarity9
Nucleotide bindingi606 – 611ATP6
Nucleotide bindingi649 – 654ATP6
Nucleotide bindingi687 – 689ATP3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • histone kinase activity (H3-T3 specific) Source: UniProtKB
  • protein kinase activity Source: UniProtKB

GO - Biological processi

  • histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore Source: UniProtKB
  • intracellular signal transduction Source: UniProtKB
  • mitotic sister chromatid cohesion Source: UniProtKB
  • protein localization to chromosome, centromeric region Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • regulation of spindle checkpoint Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS11191-MONOMER.
SignaLinkiQ8TF76.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase haspin (EC:2.7.11.1)
Alternative name(s):
Germ cell-specific gene 2 protein
H-haspin
Haploid germ cell-specific nuclear protein kinase
Gene namesi
Name:GSG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:19682. GSG2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • chromosome Source: UniProtKB-SubCell
  • cytoplasm Source: GO_Central
  • nucleus Source: UniProtKB
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi492E → A: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi511K → A: Strongly reduced enzyme activity. 1 Publication1
Mutagenesisi651H → A: Strongly reduced enzyme activity, markedly reduced affinity for histone H3. 2 Publications1
Mutagenesisi707D → L: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi709D → N: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi713G → F: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi716D → L: Markedly reduced histone H3 phosphorylation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000177602.
PharmGKBiPA134909705.

Chemistry databases

ChEMBLiCHEMBL1075163.
GuidetoPHARMACOLOGYi2028.

Polymorphism and mutation databases

BioMutaiGSG2.
DMDMi296439330.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859891 – 798Serine/threonine-protein kinase haspinAdd BLAST798

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei93Phosphoserine; by AURKBCombined sources1 Publication1
Modified residuei97PhosphothreonineCombined sources1
Modified residuei143Phosphoserine; by AURKB1 Publication1
Modified residuei147PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated on both serine and threonine residues (By similarity). Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TF76.
MaxQBiQ8TF76.
PaxDbiQ8TF76.
PeptideAtlasiQ8TF76.
PRIDEiQ8TF76.

PTM databases

iPTMnetiQ8TF76.
PhosphoSitePlusiQ8TF76.

Expressioni

Tissue specificityi

Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.2 Publications

Gene expression databases

BgeeiENSG00000177602.
CleanExiHS_GSG2.
GenevisibleiQ8TF76. HS.

Organism-specific databases

HPAiHPA027422.
HPA030698.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP90AB1P082382EBI-1237328,EBI-352572

Protein-protein interaction databases

BioGridi123814. 23 interactors.
DIPiDIP-38174N.
IntActiQ8TF76. 11 interactors.
MINTiMINT-4774775.
STRINGi9606.ENSP00000325290.

Chemistry databases

BindingDBiQ8TF76.

Structurei

Secondary structure

1798
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi452 – 466Combined sources15
Helixi475 – 478Combined sources4
Helixi481 – 485Combined sources5
Beta strandi488 – 493Combined sources6
Beta strandi496 – 503Combined sources8
Beta strandi506 – 516Combined sources11
Beta strandi518 – 521Combined sources4
Helixi529 – 544Combined sources16
Helixi545 – 547Combined sources3
Beta strandi549 – 552Combined sources4
Beta strandi554 – 556Combined sources3
Beta strandi559 – 568Combined sources10
Helixi571 – 583Combined sources13
Beta strandi599 – 606Combined sources8
Turni612 – 618Combined sources7
Helixi622 – 643Combined sources22
Helixi652 – 654Combined sources3
Beta strandi655 – 659Combined sources5
Beta strandi663 – 669Combined sources7
Beta strandi672 – 677Combined sources6
Beta strandi681 – 685Combined sources5
Beta strandi692 – 695Combined sources4
Beta strandi698 – 700Combined sources3
Helixi709 – 711Combined sources3
Helixi717 – 729Combined sources13
Helixi739 – 754Combined sources16
Beta strandi758 – 760Combined sources3
Helixi765 – 780Combined sources16
Helixi781 – 783Combined sources3
Beta strandi784 – 786Combined sources3
Helixi787 – 793Combined sources7
Helixi795 – 797Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VUWX-ray1.80A465-798[»]
2WB8X-ray2.15A452-798[»]
3DLZX-ray1.85A465-798[»]
3E7VX-ray2.00A471-798[»]
3F2NX-ray1.80A465-798[»]
3FMDX-ray2.00A470-798[»]
3IQ7X-ray2.00A465-798[»]
4OUCX-ray1.90A465-798[»]
4QTCX-ray1.40A465-798[»]
5HTBX-ray1.70A465-798[»]
5HTCX-ray1.50A465-798[»]
ProteinModelPortaliQ8TF76.
SMRiQ8TF76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TF76.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini484 – 798Protein kinasePROSITE-ProRule annotationCuratedAdd BLAST315

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2464. Eukaryota.
COG5072. LUCA.
GeneTreeiENSGT00390000013015.
HOGENOMiHOG000168256.
HOVERGENiHBG049009.
InParanoidiQ8TF76.
KOiK16315.
OMAiWGNVLLK.
OrthoDBiEOG091G07NN.
PhylomeDBiQ8TF76.
TreeFamiTF313895.

Family and domain databases

InterProiIPR024604. GSG2_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12330. DUF3635. 1 hit.
[Graphical view]
SMARTiSM01331. DUF3635. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: Q8TF76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASLPGPGS RLFRTYGAAD GRRQRRPGRE AAQWFPPQDR RRFFNSSGSS
60 70 80 90 100
DASIGDPSQS DDPDDPDDPD FPGSPVRRRR RRPGGRVPKD RPSLTVTPKR
110 120 130 140 150
WKLRARPSLT VTPRRLGLRA RPPQKCSTPC GPLRLPPFPS RDSGRLSPDL
160 170 180 190 200
SVCGQPRDGD ELGISASLFS SLASPCPGSP TPRDSVISIG TSACLVAASA
210 220 230 240 250
VPSGLHLPEV SLDRASLPCS QEEATGGAKD TRMVHQTRAS LRSVLFGLMN
260 270 280 290 300
SGTPEDSEFR ADGKNMRESC CKRKLVVGNG PEGPGLSSTG KRRATGQDSC
310 320 330 340 350
QERGLQEAVR REHQEASVPK GRIVPRGIDR LERTRSSRKS KHQEATETSL
360 370 380 390 400
LHSHRFKKGQ KLGKDSFPTQ DLTPLQNVCF WTKTRASFSF HKKKIVTDVS
410 420 430 440 450
EVCSIYTTAT SLSGSLLSEC SNRPVMNRTS GAPSSWHSSS MYLLSPLNTL
460 470 480 490 500
SISNKKASDA EKVYGECSQK GPVPFSHCLP TEKLQRCEKI GEGVFGEVFQ
510 520 530 540 550
TIADHTPVAI KIIAIEGPDL VNGSHQKTFE EILPEIIISK ELSLLSGEVC
560 570 580 590 600
NRTEGFIGLN SVHCVQGSYP PLLLKAWDHY NSTKGSANDR PDFFKDDQLF
610 620 630 640 650
IVLEFEFGGI DLEQMRTKLS SLATAKSILH QLTASLAVAE ASLRFEHRDL
660 670 680 690 700
HWGNVLLKKT SLKKLHYTLN GKSSTIPSCG LQVSIIDYTL SRLERDGIVV
710 720 730 740 750
FCDVSMDEDL FTGDGDYQFD IYRLMKKENN NRWGEYHPYS NVLWLHYLTD
760 770 780 790
KMLKQMTFKT KCNTPAMKQI KRKIQEFHRT MLNFSSATDL LCQHSLFK
Length:798
Mass (Da):88,495
Last modified:May 18, 2010 - v3
Checksum:iE0EDF8F0C58ADA31
GO
Isoform 21 Publication (identifier: Q8TF76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-314: Q → H
     315-798: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:314
Mass (Da):33,850
Checksum:i7F8CC086036ED24E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273R → S in BAB71255 (PubMed:14702039).Curated1
Sequence conflicti339K → E in AAH47457 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04054076V → E.1 PublicationCorresponds to variant rs11653889dbSNPEnsembl.1
Natural variantiVAR_04054182R → C.4 PublicationsCorresponds to variant rs9907144dbSNPEnsembl.1
Natural variantiVAR_040542145R → H.1 PublicationCorresponds to variant rs55991903dbSNPEnsembl.1
Natural variantiVAR_027405204G → D.3 PublicationsCorresponds to variant rs220462dbSNPEnsembl.1
Natural variantiVAR_040543283G → S.1 PublicationCorresponds to variant rs56224301dbSNPEnsembl.1
Natural variantiVAR_040544301Q → L.1 PublicationCorresponds to variant rs55649477dbSNPEnsembl.1
Natural variantiVAR_027406328I → T.3 PublicationsCorresponds to variant rs220461dbSNPEnsembl.1
Natural variantiVAR_027407378V → A.3 PublicationsCorresponds to variant rs3809806dbSNPEnsembl.1
Natural variantiVAR_027408422N → D.Corresponds to variant rs7223226dbSNPEnsembl.1
Natural variantiVAR_040545706M → V.1 PublicationCorresponds to variant rs56134695dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050671314Q → H in isoform 2. 1 Publication1
Alternative sequenceiVSP_050672315 – 798Missing in isoform 2. 1 PublicationAdd BLAST484

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039834 mRNA. Translation: BAB21938.3.
AF289865 mRNA. Translation: AAK30300.1.
AK056691 mRNA. Translation: BAB71255.1.
AC116914 Genomic DNA. No translation available.
BC047457 mRNA. Translation: AAH47457.1.
CCDSiCCDS11036.1. [Q8TF76-1]
RefSeqiNP_114171.2. NM_031965.2. [Q8TF76-1]
UniGeneiHs.534059.

Genome annotation databases

EnsembliENST00000325418; ENSP00000325290; ENSG00000177602. [Q8TF76-1]
GeneIDi83903.
KEGGihsa:83903.
UCSCiuc002fwp.4. human. [Q8TF76-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039834 mRNA. Translation: BAB21938.3.
AF289865 mRNA. Translation: AAK30300.1.
AK056691 mRNA. Translation: BAB71255.1.
AC116914 Genomic DNA. No translation available.
BC047457 mRNA. Translation: AAH47457.1.
CCDSiCCDS11036.1. [Q8TF76-1]
RefSeqiNP_114171.2. NM_031965.2. [Q8TF76-1]
UniGeneiHs.534059.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VUWX-ray1.80A465-798[»]
2WB8X-ray2.15A452-798[»]
3DLZX-ray1.85A465-798[»]
3E7VX-ray2.00A471-798[»]
3F2NX-ray1.80A465-798[»]
3FMDX-ray2.00A470-798[»]
3IQ7X-ray2.00A465-798[»]
4OUCX-ray1.90A465-798[»]
4QTCX-ray1.40A465-798[»]
5HTBX-ray1.70A465-798[»]
5HTCX-ray1.50A465-798[»]
ProteinModelPortaliQ8TF76.
SMRiQ8TF76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123814. 23 interactors.
DIPiDIP-38174N.
IntActiQ8TF76. 11 interactors.
MINTiMINT-4774775.
STRINGi9606.ENSP00000325290.

Chemistry databases

BindingDBiQ8TF76.
ChEMBLiCHEMBL1075163.
GuidetoPHARMACOLOGYi2028.

PTM databases

iPTMnetiQ8TF76.
PhosphoSitePlusiQ8TF76.

Polymorphism and mutation databases

BioMutaiGSG2.
DMDMi296439330.

Proteomic databases

EPDiQ8TF76.
MaxQBiQ8TF76.
PaxDbiQ8TF76.
PeptideAtlasiQ8TF76.
PRIDEiQ8TF76.

Protocols and materials databases

DNASUi83903.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325418; ENSP00000325290; ENSG00000177602. [Q8TF76-1]
GeneIDi83903.
KEGGihsa:83903.
UCSCiuc002fwp.4. human. [Q8TF76-1]

Organism-specific databases

CTDi83903.
GeneCardsiGSG2.
H-InvDBHIX0013433.
HGNCiHGNC:19682. GSG2.
HPAiHPA027422.
HPA030698.
MIMi609240. gene.
neXtProtiNX_Q8TF76.
OpenTargetsiENSG00000177602.
PharmGKBiPA134909705.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2464. Eukaryota.
COG5072. LUCA.
GeneTreeiENSGT00390000013015.
HOGENOMiHOG000168256.
HOVERGENiHBG049009.
InParanoidiQ8TF76.
KOiK16315.
OMAiWGNVLLK.
OrthoDBiEOG091G07NN.
PhylomeDBiQ8TF76.
TreeFamiTF313895.

Enzyme and pathway databases

BioCyciZFISH:HS11191-MONOMER.
SignaLinkiQ8TF76.

Miscellaneous databases

EvolutionaryTraceiQ8TF76.
GeneWikiiGSG2.
GenomeRNAii83903.
PROiQ8TF76.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177602.
CleanExiHS_GSG2.
GenevisibleiQ8TF76. HS.

Family and domain databases

InterProiIPR024604. GSG2_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12330. DUF3635. 1 hit.
[Graphical view]
SMARTiSM01331. DUF3635. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHASP_HUMAN
AccessioniPrimary (citable) accession number: Q8TF76
Secondary accession number(s): Q5U5K3, Q96MN1, Q9BXS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.