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Q8TF74 (WIPF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WAS/WASL-interacting protein family member 2
Alternative name(s):
WASP-interacting protein-related protein
WIP- and CR16-homologous protein
WIP-related protein
Gene names
Name:WIPF2
Synonyms:WICH, WIRE
ORF Names:PP10631
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. Ref.1 Ref.2

Subunit structure

Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3. Ref.1 Ref.2

Subcellular location

Cytoplasmcytoskeleton. Note: Localized to stress fibers and bundles of actin filaments. Ref.1 Ref.2

Tissue specificity

Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta. Ref.1 Ref.2

Miscellaneous

Access to the profilin-binding site is masked in the full-length protein.

Sequence similarities

Belongs to the verprolin family.

Contains 1 WH2 domain.

Sequence caution

The sequence AAQ15232.1 differs from that shown. Reason: Frameshift at position 373.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
   LigandActin-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processFc-gamma receptor signaling pathway involved in phagocytosis

Traceable author statement. Source: Reactome

innate immune response

Traceable author statement. Source: Reactome

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Traceable author statement. Source: Reactome

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GRB2P629933EBI-2850112,EBI-401755

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8TF74-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8TF74-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440WAS/WASL-interacting protein family member 2
PRO_0000065975

Regions

Domain36 – 5318WH2
Region49 – 524Binds actin Potential
Compositional bias2 – 1716Poly-Pro
Compositional bias177 – 1826Poly-Pro
Compositional bias250 – 2545Poly-Pro
Compositional bias296 – 2994Poly-Pro
Compositional bias311 – 3144Poly-Pro
Compositional bias325 – 3306Poly-Pro
Compositional bias340 – 3456Poly-Pro
Compositional bias359 – 3635Poly-Pro
Compositional bias370 – 3778Poly-Pro

Natural variations

Alternative sequence1 – 150150Missing in isoform 2.
VSP_012967

Experimental info

Sequence conflict821Q → H in CAD24007. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7386B44F995AD798

FASTA44046,289
        10         20         30         40         50         60 
MPIPPPPPPP PGPPPPPTFH QANTEQPKLS RDEQRGRGAL LQDICKGTKL KKVTNINDRS 

        70         80         90        100        110        120 
APILEKPKGS SGGYGSGGAA LQPKGGLFQG GVLKLRPVGA KDGSENLAGK PALQIPSSRA 

       130        140        150        160        170        180 
AAPRPPVSAA SGRPQDDTDS SRASLPELPR MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP 

       190        200        210        220        230        240 
PPGRRANAPP TPLPMHSSKA PAYNREKPLP PTPGQRLHPG REGPPAPPPV KPPPSPVNIR 

       250        260        270        280        290        300 
TGPSGQSLAP PPPPYRQPPG VPNGPSSPTN ESAPELPQRH NSLHRKTPGP VRGLAPPPPT 

       310        320        330        340        350        360 
SASPSLLSNR PPPPARDPPS RGAAPPPPPP VIRNGARDAP PPPPPYRMHG SEPPSRGKPP 

       370        380        390        400        410        420 
PPPSRTPAGP PPPPPPPLRN GHRDSITTVR SFLDDFESKY SFHPVEDFPA PEEYKHFQRI 

       430        440 
YPSKTNRAAR GAPPLPPILR 

« Hide

Isoform 2 [UniParc].

Checksum: B671793BBDD9B787
Show »

FASTA29030,840

References

« Hide 'large scale' references
[1]"WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation."
Kato M., Miki H., Kurita S., Endo T., Nakagawa H., Miyamoto S., Takenawa T.
Biochem. Biophys. Res. Commun. 291:41-47(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASL.
[2]"The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor."
Aspenstroem P.
Exp. Cell Res. 279:21-33(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASP; WASL; NCK2 AND PFN1.
[3]"Large-scale cDNA transfection screening for genes related to cancer development and progression."
Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. expand/collapse author list , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Cerebellum, Placenta and Tongue.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB043786 mRNA. Translation: BAB85113.1.
AJ431177 mRNA. Translation: CAD24007.1.
AF370396 mRNA. Translation: AAQ15232.1. Frameshift.
AK289578 mRNA. Translation: BAF82267.1.
AK289779 mRNA. Translation: BAF82468.1.
AK290301 mRNA. Translation: BAF82990.1.
AK315836 mRNA. Translation: BAF98727.1.
AL834239 mRNA. Translation: CAH56360.1.
BC065551 mRNA. Translation: AAH65551.1.
PIRJC7807.
RefSeqNP_573571.1. NM_133264.4.
XP_005257140.1. XM_005257083.1.
XP_005257141.1. XM_005257084.1.
UniGeneHs.421622.
Hs.705989.
Hs.731598.

3D structure databases

ProteinModelPortalQ8TF74.
SMRQ8TF74. Positions 33-65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127043. 16 interactions.
IntActQ8TF74. 4 interactions.
MINTMINT-1768510.
STRING9606.ENSP00000320924.

PTM databases

PhosphoSiteQ8TF74.

Polymorphism databases

DMDM60390855.

Proteomic databases

PaxDbQ8TF74.
PRIDEQ8TF74.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000323571; ENSP00000320924; ENSG00000171475. [Q8TF74-1]
ENST00000583130; ENSP00000462350; ENSG00000171475. [Q8TF74-1]
ENST00000585043; ENSP00000462826; ENSG00000171475. [Q8TF74-1]
GeneID147179.
KEGGhsa:147179.
UCSCuc002hug.1. human. [Q8TF74-1]

Organism-specific databases

CTD147179.
GeneCardsGC17P038375.
H-InvDBHIX0018966.
HGNCHGNC:30923. WIPF2.
HPAHPA024000.
HPA024001.
HPA024467.
MIM609692. gene.
neXtProtNX_Q8TF74.
PharmGKBPA162409210.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149184.
HOGENOMHOG000231914.
HOVERGENHBG060210.
InParanoidQ8TF74.
OMAQRVYPSK.
PhylomeDBQ8TF74.
TreeFamTF332135.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ8TF74.
BgeeQ8TF74.
CleanExHS_WIPF2.
GenevestigatorQ8TF74.

Family and domain databases

InterProIPR003124. WH2_dom.
[Graphical view]
PfamPF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiWIPF2.
GenomeRNAi147179.
NextBio85562.
PROQ8TF74.
SOURCESearch...

Entry information

Entry nameWIPF2_HUMAN
AccessionPrimary (citable) accession number: Q8TF74
Secondary accession number(s): A8K0L3 expand/collapse secondary AC list , Q658J8, Q71RE1, Q8TE44
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2002
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM