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Q8TF74

- WIPF2_HUMAN

UniProt

Q8TF74 - WIPF2_HUMAN

Protein

WAS/WASL-interacting protein family member 2

Gene

WIPF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
    2. innate immune response Source: Reactome

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_160086. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WAS/WASL-interacting protein family member 2
    Alternative name(s):
    WASP-interacting protein-related protein
    WIP- and CR16-homologous protein
    WIP-related protein
    Gene namesi
    Name:WIPF2
    Synonyms:WICH, WIRE
    ORF Names:PP10631
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:30923. WIPF2.

    Subcellular locationi

    Cytoplasmcytoskeleton 2 Publications
    Note: Localized to stress fibers and bundles of actin filaments.

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. cytosol Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162409210.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440WAS/WASL-interacting protein family member 2PRO_0000065975Add
    BLAST

    Proteomic databases

    MaxQBiQ8TF74.
    PaxDbiQ8TF74.
    PRIDEiQ8TF74.

    PTM databases

    PhosphoSiteiQ8TF74.

    Expressioni

    Tissue specificityi

    Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta.2 Publications

    Gene expression databases

    ArrayExpressiQ8TF74.
    BgeeiQ8TF74.
    CleanExiHS_WIPF2.
    GenevestigatoriQ8TF74.

    Organism-specific databases

    HPAiHPA024000.
    HPA024001.
    HPA024467.

    Interactioni

    Subunit structurei

    Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GRB2P629933EBI-2850112,EBI-401755

    Protein-protein interaction databases

    BioGridi127043. 16 interactions.
    IntActiQ8TF74. 4 interactions.
    MINTiMINT-1768510.
    STRINGi9606.ENSP00000320924.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TF74.
    SMRiQ8TF74. Positions 33-65.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 5318WH2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 524Binds actinSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 1716Poly-ProAdd
    BLAST
    Compositional biasi177 – 1826Poly-Pro
    Compositional biasi250 – 2545Poly-Pro
    Compositional biasi296 – 2994Poly-Pro
    Compositional biasi311 – 3144Poly-Pro
    Compositional biasi325 – 3306Poly-Pro
    Compositional biasi340 – 3456Poly-Pro
    Compositional biasi359 – 3635Poly-Pro
    Compositional biasi370 – 3778Poly-Pro

    Sequence similaritiesi

    Belongs to the verprolin family.Curated
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149184.
    HOGENOMiHOG000231914.
    HOVERGENiHBG060210.
    InParanoidiQ8TF74.
    OMAiQRVYPSK.
    PhylomeDBiQ8TF74.
    TreeFamiTF332135.

    Family and domain databases

    InterProiIPR003124. WH2_dom.
    IPR028294. WIPF2.
    [Graphical view]
    PANTHERiPTHR23202:SF3. PTHR23202:SF3. 1 hit.
    PfamiPF02205. WH2. 1 hit.
    [Graphical view]
    SMARTiSM00246. WH2. 1 hit.
    [Graphical view]
    PROSITEiPS51082. WH2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8TF74-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPIPPPPPPP PGPPPPPTFH QANTEQPKLS RDEQRGRGAL LQDICKGTKL    50
    KKVTNINDRS APILEKPKGS SGGYGSGGAA LQPKGGLFQG GVLKLRPVGA 100
    KDGSENLAGK PALQIPSSRA AAPRPPVSAA SGRPQDDTDS SRASLPELPR 150
    MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP PPGRRANAPP TPLPMHSSKA 200
    PAYNREKPLP PTPGQRLHPG REGPPAPPPV KPPPSPVNIR TGPSGQSLAP 250
    PPPPYRQPPG VPNGPSSPTN ESAPELPQRH NSLHRKTPGP VRGLAPPPPT 300
    SASPSLLSNR PPPPARDPPS RGAAPPPPPP VIRNGARDAP PPPPPYRMHG 350
    SEPPSRGKPP PPPSRTPAGP PPPPPPPLRN GHRDSITTVR SFLDDFESKY 400
    SFHPVEDFPA PEEYKHFQRI YPSKTNRAAR GAPPLPPILR 440
    Length:440
    Mass (Da):46,289
    Last modified:June 1, 2002 - v1
    Checksum:i7386B44F995AD798
    GO
    Isoform 2 (identifier: Q8TF74-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-150: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:290
    Mass (Da):30,840
    Checksum:iB671793BBDD9B787
    GO

    Sequence cautioni

    The sequence AAQ15232.1 differs from that shown. Reason: Frameshift at position 373.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821Q → H in CAD24007. (PubMed:12213210)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 150150Missing in isoform 2. 1 PublicationVSP_012967Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB043786 mRNA. Translation: BAB85113.1.
    AJ431177 mRNA. Translation: CAD24007.1.
    AF370396 mRNA. Translation: AAQ15232.1. Frameshift.
    AK289578 mRNA. Translation: BAF82267.1.
    AK289779 mRNA. Translation: BAF82468.1.
    AK290301 mRNA. Translation: BAF82990.1.
    AK315836 mRNA. Translation: BAF98727.1.
    AL834239 mRNA. Translation: CAH56360.1.
    BC065551 mRNA. Translation: AAH65551.1.
    CCDSiCCDS11364.1. [Q8TF74-1]
    PIRiJC7807.
    RefSeqiNP_573571.1. NM_133264.4. [Q8TF74-1]
    XP_005257140.1. XM_005257083.1. [Q8TF74-1]
    XP_005257141.1. XM_005257084.1. [Q8TF74-1]
    UniGeneiHs.421622.
    Hs.705989.
    Hs.731598.

    Genome annotation databases

    EnsembliENST00000323571; ENSP00000320924; ENSG00000171475. [Q8TF74-1]
    ENST00000583130; ENSP00000462350; ENSG00000171475. [Q8TF74-1]
    ENST00000585043; ENSP00000462826; ENSG00000171475. [Q8TF74-1]
    GeneIDi147179.
    KEGGihsa:147179.
    UCSCiuc002hug.1. human. [Q8TF74-1]

    Polymorphism databases

    DMDMi60390855.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB043786 mRNA. Translation: BAB85113.1 .
    AJ431177 mRNA. Translation: CAD24007.1 .
    AF370396 mRNA. Translation: AAQ15232.1 . Frameshift.
    AK289578 mRNA. Translation: BAF82267.1 .
    AK289779 mRNA. Translation: BAF82468.1 .
    AK290301 mRNA. Translation: BAF82990.1 .
    AK315836 mRNA. Translation: BAF98727.1 .
    AL834239 mRNA. Translation: CAH56360.1 .
    BC065551 mRNA. Translation: AAH65551.1 .
    CCDSi CCDS11364.1. [Q8TF74-1 ]
    PIRi JC7807.
    RefSeqi NP_573571.1. NM_133264.4. [Q8TF74-1 ]
    XP_005257140.1. XM_005257083.1. [Q8TF74-1 ]
    XP_005257141.1. XM_005257084.1. [Q8TF74-1 ]
    UniGenei Hs.421622.
    Hs.705989.
    Hs.731598.

    3D structure databases

    ProteinModelPortali Q8TF74.
    SMRi Q8TF74. Positions 33-65.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 127043. 16 interactions.
    IntActi Q8TF74. 4 interactions.
    MINTi MINT-1768510.
    STRINGi 9606.ENSP00000320924.

    PTM databases

    PhosphoSitei Q8TF74.

    Polymorphism databases

    DMDMi 60390855.

    Proteomic databases

    MaxQBi Q8TF74.
    PaxDbi Q8TF74.
    PRIDEi Q8TF74.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000323571 ; ENSP00000320924 ; ENSG00000171475 . [Q8TF74-1 ]
    ENST00000583130 ; ENSP00000462350 ; ENSG00000171475 . [Q8TF74-1 ]
    ENST00000585043 ; ENSP00000462826 ; ENSG00000171475 . [Q8TF74-1 ]
    GeneIDi 147179.
    KEGGi hsa:147179.
    UCSCi uc002hug.1. human. [Q8TF74-1 ]

    Organism-specific databases

    CTDi 147179.
    GeneCardsi GC17P038375.
    H-InvDB HIX0018966.
    HGNCi HGNC:30923. WIPF2.
    HPAi HPA024000.
    HPA024001.
    HPA024467.
    MIMi 609692. gene.
    neXtProti NX_Q8TF74.
    PharmGKBi PA162409210.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG149184.
    HOGENOMi HOG000231914.
    HOVERGENi HBG060210.
    InParanoidi Q8TF74.
    OMAi QRVYPSK.
    PhylomeDBi Q8TF74.
    TreeFami TF332135.

    Enzyme and pathway databases

    Reactomei REACT_160086. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    GeneWikii WIPF2.
    GenomeRNAii 147179.
    NextBioi 85562.
    PROi Q8TF74.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8TF74.
    Bgeei Q8TF74.
    CleanExi HS_WIPF2.
    Genevestigatori Q8TF74.

    Family and domain databases

    InterProi IPR003124. WH2_dom.
    IPR028294. WIPF2.
    [Graphical view ]
    PANTHERi PTHR23202:SF3. PTHR23202:SF3. 1 hit.
    Pfami PF02205. WH2. 1 hit.
    [Graphical view ]
    SMARTi SM00246. WH2. 1 hit.
    [Graphical view ]
    PROSITEi PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation."
      Kato M., Miki H., Kurita S., Endo T., Nakagawa H., Miyamoto S., Takenawa T.
      Biochem. Biophys. Res. Commun. 291:41-47(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASL.
    2. "The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor."
      Aspenstroem P.
      Exp. Cell Res. 279:21-33(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASP; WASL; NCK2 AND PFN1.
    3. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
      Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
      , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
      Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain, Cerebellum, Placenta and Tongue.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiWIPF2_HUMAN
    AccessioniPrimary (citable) accession number: Q8TF74
    Secondary accession number(s): A8K0L3
    , Q658J8, Q71RE1, Q8TE44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Access to the profilin-binding site is masked in the full-length protein.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3