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Q8TF74

- WIPF2_HUMAN

UniProt

Q8TF74 - WIPF2_HUMAN

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Protein

WAS/WASL-interacting protein family member 2

Gene
WIPF2, WICH, WIRE, PP10631
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  2. innate immune response Source: Reactome
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Names & Taxonomyi

Protein namesi
Recommended name:
WAS/WASL-interacting protein family member 2
Alternative name(s):
WASP-interacting protein-related protein
WIP- and CR16-homologous protein
WIP-related protein
Gene namesi
Name:WIPF2
Synonyms:WICH, WIRE
ORF Names:PP10631
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:30923. WIPF2.

Subcellular locationi

Cytoplasmcytoskeleton
Note: Localized to stress fibers and bundles of actin filaments.2 Publications

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-SubCell
  2. cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162409210.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440WAS/WASL-interacting protein family member 2PRO_0000065975Add
BLAST

Proteomic databases

MaxQBiQ8TF74.
PaxDbiQ8TF74.
PRIDEiQ8TF74.

PTM databases

PhosphoSiteiQ8TF74.

Expressioni

Tissue specificityi

Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta.2 Publications

Gene expression databases

ArrayExpressiQ8TF74.
BgeeiQ8TF74.
CleanExiHS_WIPF2.
GenevestigatoriQ8TF74.

Organism-specific databases

HPAiHPA024000.
HPA024001.
HPA024467.

Interactioni

Subunit structurei

Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GRB2P629933EBI-2850112,EBI-401755

Protein-protein interaction databases

BioGridi127043. 16 interactions.
IntActiQ8TF74. 4 interactions.
MINTiMINT-1768510.
STRINGi9606.ENSP00000320924.

Structurei

3D structure databases

ProteinModelPortaliQ8TF74.
SMRiQ8TF74. Positions 33-65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 5318WH2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 524Binds actin Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 1716Poly-ProAdd
BLAST
Compositional biasi177 – 1826Poly-Pro
Compositional biasi250 – 2545Poly-Pro
Compositional biasi296 – 2994Poly-Pro
Compositional biasi311 – 3144Poly-Pro
Compositional biasi325 – 3306Poly-Pro
Compositional biasi340 – 3456Poly-Pro
Compositional biasi359 – 3635Poly-Pro
Compositional biasi370 – 3778Poly-Pro

Sequence similaritiesi

Belongs to the verprolin family.
Contains 1 WH2 domain.

Phylogenomic databases

eggNOGiNOG149184.
HOGENOMiHOG000231914.
HOVERGENiHBG060210.
InParanoidiQ8TF74.
OMAiQRVYPSK.
PhylomeDBiQ8TF74.
TreeFamiTF332135.

Family and domain databases

InterProiIPR003124. WH2_dom.
IPR028294. WIPF2.
[Graphical view]
PANTHERiPTHR23202:SF3. PTHR23202:SF3. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8TF74-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPIPPPPPPP PGPPPPPTFH QANTEQPKLS RDEQRGRGAL LQDICKGTKL    50
KKVTNINDRS APILEKPKGS SGGYGSGGAA LQPKGGLFQG GVLKLRPVGA 100
KDGSENLAGK PALQIPSSRA AAPRPPVSAA SGRPQDDTDS SRASLPELPR 150
MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP PPGRRANAPP TPLPMHSSKA 200
PAYNREKPLP PTPGQRLHPG REGPPAPPPV KPPPSPVNIR TGPSGQSLAP 250
PPPPYRQPPG VPNGPSSPTN ESAPELPQRH NSLHRKTPGP VRGLAPPPPT 300
SASPSLLSNR PPPPARDPPS RGAAPPPPPP VIRNGARDAP PPPPPYRMHG 350
SEPPSRGKPP PPPSRTPAGP PPPPPPPLRN GHRDSITTVR SFLDDFESKY 400
SFHPVEDFPA PEEYKHFQRI YPSKTNRAAR GAPPLPPILR 440
Length:440
Mass (Da):46,289
Last modified:June 1, 2002 - v1
Checksum:i7386B44F995AD798
GO
Isoform 2 (identifier: Q8TF74-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.

Note: No experimental confirmation available.

Show »
Length:290
Mass (Da):30,840
Checksum:iB671793BBDD9B787
GO

Sequence cautioni

The sequence AAQ15232.1 differs from that shown. Reason: Frameshift at position 373.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 150150Missing in isoform 2. VSP_012967Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821Q → H in CAD24007. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB043786 mRNA. Translation: BAB85113.1.
AJ431177 mRNA. Translation: CAD24007.1.
AF370396 mRNA. Translation: AAQ15232.1. Frameshift.
AK289578 mRNA. Translation: BAF82267.1.
AK289779 mRNA. Translation: BAF82468.1.
AK290301 mRNA. Translation: BAF82990.1.
AK315836 mRNA. Translation: BAF98727.1.
AL834239 mRNA. Translation: CAH56360.1.
BC065551 mRNA. Translation: AAH65551.1.
CCDSiCCDS11364.1. [Q8TF74-1]
PIRiJC7807.
RefSeqiNP_573571.1. NM_133264.4. [Q8TF74-1]
XP_005257140.1. XM_005257083.1. [Q8TF74-1]
XP_005257141.1. XM_005257084.1. [Q8TF74-1]
UniGeneiHs.421622.
Hs.705989.
Hs.731598.

Genome annotation databases

EnsembliENST00000323571; ENSP00000320924; ENSG00000171475. [Q8TF74-1]
ENST00000583130; ENSP00000462350; ENSG00000171475. [Q8TF74-1]
ENST00000585043; ENSP00000462826; ENSG00000171475. [Q8TF74-1]
GeneIDi147179.
KEGGihsa:147179.
UCSCiuc002hug.1. human. [Q8TF74-1]

Polymorphism databases

DMDMi60390855.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB043786 mRNA. Translation: BAB85113.1 .
AJ431177 mRNA. Translation: CAD24007.1 .
AF370396 mRNA. Translation: AAQ15232.1 . Frameshift.
AK289578 mRNA. Translation: BAF82267.1 .
AK289779 mRNA. Translation: BAF82468.1 .
AK290301 mRNA. Translation: BAF82990.1 .
AK315836 mRNA. Translation: BAF98727.1 .
AL834239 mRNA. Translation: CAH56360.1 .
BC065551 mRNA. Translation: AAH65551.1 .
CCDSi CCDS11364.1. [Q8TF74-1 ]
PIRi JC7807.
RefSeqi NP_573571.1. NM_133264.4. [Q8TF74-1 ]
XP_005257140.1. XM_005257083.1. [Q8TF74-1 ]
XP_005257141.1. XM_005257084.1. [Q8TF74-1 ]
UniGenei Hs.421622.
Hs.705989.
Hs.731598.

3D structure databases

ProteinModelPortali Q8TF74.
SMRi Q8TF74. Positions 33-65.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 127043. 16 interactions.
IntActi Q8TF74. 4 interactions.
MINTi MINT-1768510.
STRINGi 9606.ENSP00000320924.

PTM databases

PhosphoSitei Q8TF74.

Polymorphism databases

DMDMi 60390855.

Proteomic databases

MaxQBi Q8TF74.
PaxDbi Q8TF74.
PRIDEi Q8TF74.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000323571 ; ENSP00000320924 ; ENSG00000171475 . [Q8TF74-1 ]
ENST00000583130 ; ENSP00000462350 ; ENSG00000171475 . [Q8TF74-1 ]
ENST00000585043 ; ENSP00000462826 ; ENSG00000171475 . [Q8TF74-1 ]
GeneIDi 147179.
KEGGi hsa:147179.
UCSCi uc002hug.1. human. [Q8TF74-1 ]

Organism-specific databases

CTDi 147179.
GeneCardsi GC17P038375.
H-InvDB HIX0018966.
HGNCi HGNC:30923. WIPF2.
HPAi HPA024000.
HPA024001.
HPA024467.
MIMi 609692. gene.
neXtProti NX_Q8TF74.
PharmGKBi PA162409210.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG149184.
HOGENOMi HOG000231914.
HOVERGENi HBG060210.
InParanoidi Q8TF74.
OMAi QRVYPSK.
PhylomeDBi Q8TF74.
TreeFami TF332135.

Enzyme and pathway databases

Reactomei REACT_160086. Regulation of actin dynamics for phagocytic cup formation.

Miscellaneous databases

GeneWikii WIPF2.
GenomeRNAii 147179.
NextBioi 85562.
PROi Q8TF74.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8TF74.
Bgeei Q8TF74.
CleanExi HS_WIPF2.
Genevestigatori Q8TF74.

Family and domain databases

InterProi IPR003124. WH2_dom.
IPR028294. WIPF2.
[Graphical view ]
PANTHERi PTHR23202:SF3. PTHR23202:SF3. 1 hit.
Pfami PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00246. WH2. 1 hit.
[Graphical view ]
PROSITEi PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation."
    Kato M., Miki H., Kurita S., Endo T., Nakagawa H., Miyamoto S., Takenawa T.
    Biochem. Biophys. Res. Commun. 291:41-47(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASL.
  2. "The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor."
    Aspenstroem P.
    Exp. Cell Res. 279:21-33(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASP; WASL; NCK2 AND PFN1.
  3. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain, Cerebellum, Placenta and Tongue.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiWIPF2_HUMAN
AccessioniPrimary (citable) accession number: Q8TF74
Secondary accession number(s): A8K0L3
, Q658J8, Q71RE1, Q8TE44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: June 1, 2002
Last modified: September 3, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Access to the profilin-binding site is masked in the full-length protein.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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