Q8TF74 (WIPF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WAS/WASL-interacting protein family member 2

Alternative name(s):
WASP-interacting protein-related protein
WIP- and CR16-homologous protein
WIP-related protein

Gene names

Name:	WIPF2
Synonyms:	WICH, WIRE
ORF Names:	PP10631

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. Ref.1 Ref.2
Subunit structure	Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3. Ref.1 Ref.2
Subcellular location	Cytoplasm › cytoskeleton. Note: Localized to stress fibers and bundles of actin filaments. Ref.1 Ref.2
Tissue specificity	Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta. Ref.1 Ref.2
Miscellaneous	Access to the profilin-binding site is masked in the full-length protein.
Sequence similarities	Belongs to the verprolin family. Contains 1 WH2 domain.
Sequence caution	The sequence AAQ15232.1 differs from that shown. Reason: Frameshift at position 373.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Fc-gamma receptor signaling pathway involved in phagocytosis Traceable author statement. Source: Reactome innate immune response Traceable author statement. Source: Reactome
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRB2	P62993	3	EBI-2850112,EBI-401755

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TF74-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TF74-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	WAS/WASL-interacting protein family member 2		PRO_0000065975
Regions
	Domain	36 – 53	18	WH2
	Region	49 – 52	4	Binds actin Potential
	Compositional bias	2 – 17	16	Poly-Pro
	Compositional bias	177 – 182	6	Poly-Pro
	Compositional bias	250 – 254	5	Poly-Pro
	Compositional bias	296 – 299	4	Poly-Pro
	Compositional bias	311 – 314	4	Poly-Pro
	Compositional bias	325 – 330	6	Poly-Pro
	Compositional bias	340 – 345	6	Poly-Pro
	Compositional bias	359 – 363	5	Poly-Pro
	Compositional bias	370 – 377	8	Poly-Pro
Natural variations
	Alternative sequence	1 – 150	150	Missing in isoform 2.		VSP_012967
Experimental info
	Sequence conflict	82	1	Q → H in CAD24007. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 7386B44F995AD798	FASTA	440	46,289
	10 20 30 40 50 60 MPIPPPPPPP PGPPPPPTFH QANTEQPKLS RDEQRGRGAL LQDICKGTKL KKVTNINDRS 70 80 90 100 110 120 APILEKPKGS SGGYGSGGAA LQPKGGLFQG GVLKLRPVGA KDGSENLAGK PALQIPSSRA 130 140 150 160 170 180 AAPRPPVSAA SGRPQDDTDS SRASLPELPR MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP 190 200 210 220 230 240 PPGRRANAPP TPLPMHSSKA PAYNREKPLP PTPGQRLHPG REGPPAPPPV KPPPSPVNIR 250 260 270 280 290 300 TGPSGQSLAP PPPPYRQPPG VPNGPSSPTN ESAPELPQRH NSLHRKTPGP VRGLAPPPPT 310 320 330 340 350 360 SASPSLLSNR PPPPARDPPS RGAAPPPPPP VIRNGARDAP PPPPPYRMHG SEPPSRGKPP 370 380 390 400 410 420 PPPSRTPAGP PPPPPPPLRN GHRDSITTVR SFLDDFESKY SFHPVEDFPA PEEYKHFQRI 430 440 YPSKTNRAAR GAPPLPPILR
	Isoform 2 [UniParc]. Checksum: B671793BBDD9B787	FASTA	290	30,840
	10 20 30 40 50 60 MQRPSLPDLS RPNTTSSTGM KHSSSAPPPP PPGRRANAPP TPLPMHSSKA PAYNREKPLP 70 80 90 100 110 120 PTPGQRLHPG REGPPAPPPV KPPPSPVNIR TGPSGQSLAP PPPPYRQPPG VPNGPSSPTN 130 140 150 160 170 180 ESAPELPQRH NSLHRKTPGP VRGLAPPPPT SASPSLLSNR PPPPARDPPS RGAAPPPPPP 190 200 210 220 230 240 VIRNGARDAP PPPPPYRMHG SEPPSRGKPP PPPSRTPAGP PPPPPPPLRN GHRDSITTVR 250 260 270 280 290 SFLDDFESKY SFHPVEDFPA PEEYKHFQRI YPSKTNRAAR GAPPLPPILR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation." Kato M., Miki H., Kurita S., Endo T., Nakagawa H., Miyamoto S., Takenawa T. Biochem. Biophys. Res. Commun. 291:41-47(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASL.
[2]	"The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor." Aspenstroem P. Exp. Cell Res. 279:21-33(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH WASP; WASL; NCK2 AND PFN1.
[3]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain, Cerebellum, Placenta and Tongue.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB043786 mRNA. Translation: BAB85113.1. AJ431177 mRNA. Translation: CAD24007.1. AF370396 mRNA. Translation: AAQ15232.1. Frameshift. AK289578 mRNA. Translation: BAF82267.1. AK289779 mRNA. Translation: BAF82468.1. AK290301 mRNA. Translation: BAF82990.1. AK315836 mRNA. Translation: BAF98727.1. AL834239 mRNA. Translation: CAH56360.1. BC065551 mRNA. Translation: AAH65551.1.
IPI	IPI00435947. IPI00552647.
PIR	JC7807.
RefSeq	NP_573571.1. NM_133264.4.
UniGene	Hs.421622. Hs.705989. Hs.731598.
3D structure databases
ProteinModelPortal	Q8TF74.
SMR	Q8TF74. Positions 33-65.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TF74. 4 interactions.
MINT	MINT-1768510.
STRING	9606.ENSP00000320924.
PTM databases
PhosphoSite	Q8TF74.
Polymorphism databases
DMDM	60390855.
Proteomic databases
PaxDb	Q8TF74.
PRIDE	Q8TF74.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000323571; ENSP00000320924; ENSG00000171475. ENST00000494757; ENSP00000463383; ENSG00000171475. ENST00000583130; ENSP00000462350; ENSG00000171475. ENST00000585043; ENSP00000462826; ENSG00000171475.
GeneID	147179.
KEGG	hsa:147179.
UCSC	uc002hug.1. human.
Organism-specific databases
CTD	147179.
GeneCards	GC17P038375.
H-InvDB	HIX0018966.
HGNC	HGNC:30923. WIPF2.
HPA	HPA024000. HPA024001. HPA024467.
MIM	609692. gene.
neXtProt	NX_Q8TF74.
PharmGKB	PA162409210.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG149184.
HOGENOM	HOG000231914.
HOVERGEN	HBG060210.
InParanoid	Q8TF74.
OMA	AQAYNRE.
OrthoDB	EOG40P482.
PhylomeDB	Q8TF74.
Gene expression databases
ArrayExpress	Q8TF74.
Bgee	Q8TF74.
CleanEx	HS_WIPF2.
Genevestigator	Q8TF74.
Family and domain databases
InterPro	IPR003124. WH2_dom. [Graphical view]
Pfam	PF02205. WH2. 1 hit. [Graphical view]
SMART	SM00246. WH2. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	147179.
NextBio	85562.
SOURCE	Search...

Entry information

Entry name

WIPF2_HUMAN

Accession

Primary (citable) accession number: Q8TF74
Secondary accession number(s): A8K0L3 , Q658J8, Q71RE1, Q8TE44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.