Q8TF71 (MOT10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monocarboxylate transporter 10
Short name=MCT 10

Alternative name(s):
Aromatic amino acid transporter 1
Solute carrier family 16 member 10
T-type amino acid transporter 1

Gene names

Name:	SLC16A10
Synonyms:	MCT10, TAT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent transporter that mediates the update of aromatic acid. Can function as a net efflux pathway for aromatic amino acids in the basosolateral epithelial cells By similarity. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane By similarity.
Tissue specificity	Strongly expressed in kidney and skeletal muscle and at lower level in placenta and heart. Ref.1
Post-translational modification	Not N-glycosylated By similarity.
Sequence similarities	Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]
Sequence caution	The sequence AAH17968.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid transport Traceable author statement PubMed 15918515. Source: HGNC transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	basolateral plasma membrane Traceable author statement PubMed 15918515. Source: HGNC integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	Monocarboxylate transporter 10		PRO_0000314253
Regions
	Topological domain	1 – 66	66	Extracellular Potential
	Transmembrane	67 – 87	21	Helical; Potential
	Topological domain	88 – 114	27	Cytoplasmic Potential
	Transmembrane	115 – 135	21	Helical; Potential
	Topological domain	136 – 144	9	Extracellular Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Topological domain	166 – 171	6	Cytoplasmic Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Topological domain	193 – 200	8	Extracellular Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Topological domain	222 – 228	7	Cytoplasmic Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Topological domain	250 – 291	42	Extracellular Potential
	Transmembrane	292 – 312	21	Helical; Potential
	Topological domain	313 – 329	17	Cytoplasmic Potential
	Transmembrane	330 – 350	21	Helical; Potential
	Topological domain	351	1	Extracellular Potential
	Transmembrane	352 – 372	21	Helical; Potential
	Topological domain	373 – 396	24	Cytoplasmic Potential
	Transmembrane	397 – 417	21	Helical; Potential
	Topological domain	418 – 419	2	Extracellular Potential
	Transmembrane	420 – 440	21	Helical; Potential
	Topological domain	441 – 451	11	Cytoplasmic Potential
	Transmembrane	452 – 472	21	Helical; Potential
	Topological domain	473 – 515	43	Extracellular Potential
Amino acid modifications
	Modified residue	269	1	Phosphoserine By similarity
	Modified residue	498	1	Phosphoserine Ref.6
	Modified residue	504	1	Phosphoserine By similarity
Natural variations
	Natural variant	508	1	K → Q. Corresponds to variant rs17072442 [ dbSNP | Ensembl ].		VAR_037874
Experimental info
	Sequence conflict	422	1	F → L in AAH17968. Ref.5
	Sequence conflict	431	1	M → T in BAD18768. Ref.2
	Sequence conflict	443	1	D → G in BAD18768. Ref.2
	Sequence conflict	508	1	K → E in AAH17968. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q8TF71 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A870E4DDC26B918E	FASTA	515	55,493
	10 20 30 40 50 60 MVLSQEEPDS ARGTSEAQPL GPAPTGAAPP PGPGPSDSPE AAVEKVEVEL AGPATAEPHE 70 80 90 100 110 120 PPEPPEGGWG WLVMLAAMWC NGSVFGIQNA CGVLFVSMLE TFGSKDDDKM VFKTAWVGSL 130 140 150 160 170 180 SMGMIFFCCP IVSVFTDLFG CRKTAVVGAA VGFVGLMSSS FVSSIEPLYL TYGIIFACGC 190 200 210 220 230 240 SFAYQPSLVI LGHYFKKRLG LVNGIVTAGS SVFTILLPLL LRVLIDSVGL FYTLRVLCIF 250 260 270 280 290 300 MFVLFLAGFT YRPLATSTKD KESGGSGSSL FSRKKFSPPK KIFNFAIFKV TAYAVWAVGI 310 320 330 340 350 360 PLALFGYFVP YVHLMKHVNE RFQDEKNKEV VLMCIGVTSG VGRLLFGRIA DYVPGVKKVY 370 380 390 400 410 420 LQVLSFFFIG LMSMMIPLCS IFGALIAVCL IMGLFDGCFI SIMAPIAFEL VGAQDVSQAI 430 440 450 460 470 480 GFLLGFMSIP MTVGPPIAGL LRDKLGSYDV AFYLAGVPPL IGGAVLCFIP WIHSKKQREI 490 500 510 SKTTGKEKME KMLENQNSLL SSSSGMFKKE SDSII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human T-type amino acid transporter-1: characterization, gene organization, and chromosomal location." Kim D.K., Kanai Y., Matsuo H., Kim J.Y., Chairoungdua A., Kobayashi Y., Enomoto A., Cha S.H., Goya T., Endou H. Genomics 79:95-103(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION. Tissue: Thymus.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta, Prostate and Testis.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-498, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB057445 mRNA. Translation: BAB84670.1. AK126183 mRNA. Translation: BAG54292.1. AK172789 mRNA. Translation: BAD18768.1. AL360227 Genomic DNA. Translation: CAH73732.1. CH471051 Genomic DNA. Translation: EAW48298.1. BC017968 mRNA. Translation: AAH17968.1. Different initiation. BC066985 mRNA. Translation: AAH66985.1.
IPI	IPI00152879.
RefSeq	NP_061063.2. NM_018593.4.
UniGene	Hs.591327.
3D structure databases
ProteinModelPortal	Q8TF71.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000357844.
Protein family/group databases
TCDB	2.A.1.13.8. major facilitator superfamily (MFS).
PTM databases
PhosphoSite	Q8TF71.
Polymorphism databases
DMDM	74751472.
Proteomic databases
PaxDb	Q8TF71.
PRIDE	Q8TF71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368851; ENSP00000357844; ENSG00000112394.
GeneID	117247.
KEGG	hsa:117247.
UCSC	uc003pur.4. human.
Organism-specific databases
CTD	117247.
GeneCards	GC06P111454.
HGNC	HGNC:17027. SLC16A10.
HPA	HPA016860.
MIM	607550. gene.
neXtProt	NX_Q8TF71.
PharmGKB	PA38197.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000046644.
HOVERGEN	HBG006387.
InParanoid	Q8TF71.
KO	K08187.
OMA	LMKHVNE.
OrthoDB	EOG4SBDZ5.
PhylomeDB	Q8TF71.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	Q8TF71.
Bgee	Q8TF71.
CleanEx	HS_SLC16A10.
Genevestigator	Q8TF71.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	117247.
NextBio	80178.
SOURCE	Search...

Entry information

Entry name

MOT10_HUMAN

Accession

Primary (citable) accession number: Q8TF71
Secondary accession number(s): B3KWY0, Q6ZMG0, Q8WVI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2002
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.