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Protein

DIS3-like exonuclease 1

Gene

DIS3L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative cytoplasm-specific catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA.2 Publications

Cofactori

Mg2+2 Publications

GO - Molecular functioni

  • 3'-5'-exoribonuclease activity Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA catabolic process Source: GO_Central
  • RNA phosphodiester bond hydrolysis, exonucleolytic Source: GOC
  • rRNA catabolic process Source: UniProtKB
  • rRNA processing Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DIS3-like exonuclease 1 (EC:3.1.13.-)
Gene namesi
Name:DIS3L
Synonyms:DIS3L1, KIAA1955
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:28698. DIS3L.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Exosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 621D → N: No change of exonuclease activity. 1 Publication
Mutagenesisi166 – 1661D → N: No change of exonuclease activity. 1 Publication
Mutagenesisi486 – 4861D → N: Complete loss of exonuclease activity. 2 Publications

Organism-specific databases

PharmGKBiPA162383675.

Polymorphism and mutation databases

BioMutaiDIS3L.
DMDMi166201903.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054DIS3-like exonuclease 1PRO_0000314810Add
BLAST

Proteomic databases

MaxQBiQ8TF46.
PaxDbiQ8TF46.
PeptideAtlasiQ8TF46.
PRIDEiQ8TF46.

PTM databases

PhosphoSiteiQ8TF46.

Expressioni

Gene expression databases

BgeeiQ8TF46.
CleanExiHS_DIS3L.
ExpressionAtlasiQ8TF46. baseline and differential.
GenevestigatoriQ8TF46.

Organism-specific databases

HPAiHPA041805.

Interactioni

Subunit structurei

Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms.

Binary interactionsi

WithEntry#Exp.IntActNotes
EXOSC2Q138682EBI-3895807,EBI-301735
EXOSC3Q9NQT53EBI-3672244,EBI-371866

Protein-protein interaction databases

BioGridi125453. 21 interactions.
IntActiQ8TF46. 15 interactions.
STRINGi9606.ENSP00000321711.

Structurei

3D structure databases

ProteinModelPortaliQ8TF46.
SMRiQ8TF46. Positions 9-955.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNR ribonuclease family.Curated

Phylogenomic databases

eggNOGiCOG0557.
GeneTreeiENSGT00530000063106.
HOGENOMiHOG000191945.
HOVERGENiHBG059397.
InParanoidiQ8TF46.
KOiK18681.
OMAiIPRFGIK.
OrthoDBiEOG77T13R.
PhylomeDBiQ8TF46.
TreeFamiTF105755.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR022966. RNase_II/R_CS.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
PROSITEiPS01175. RIBONUCLEASE_II. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TF46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQKREKVLL LRTFQGRTLR IVREHYLRPC VPCHSPLCPQ PAACSHDGKL
60 70 80 90 100
LSSDVTHYVI PDWKVVQDYL EILEFPELKG IIFMQTACQA VQHQRGRRQY
110 120 130 140 150
NKLRNLLKDA RHDCILFANE FQQCCYLPRE RGESMEKWQT RSIYNAAVWY
160 170 180 190 200
YHHCQDRMPI VMVTEDEEAI QQYGSETEGV FVITFKNYLD NFWPDLKAAH
210 220 230 240 250
ELCDSILQSR RERENESQES HGKEYPEHLP LEVLEAGIKS GRYIQGILNV
260 270 280 290 300
NKHRAQIEAF VRLQGASSKD SDLVSDILIH GMKARNRSIH GDVVVVELLP
310 320 330 340 350
KNEWKGRTVA LCENDCDDKA SGESPSEPMP TGRVVGILQK NWRDYVVTFP
360 370 380 390 400
SKEEVQSQGK NAQKILVTPW DYRIPKIRIS TQQAETLQDF RVVVRIDSWE
410 420 430 440 450
STSVYPNGHF VRVLGRIGDL EGEIATILVE NSISVIPFSE AQMCEMPVNT
460 470 480 490 500
PESPWKVSPE EEQKRKDLRK SHLVFSIDPK GCEDVDDTLS VRTLNNGNLE
510 520 530 540 550
LGVHIADVTH FVAPNSYIDI EARTRATTYY LADRRYDMLP SVLSADLCSL
560 570 580 590 600
LGGVDRYAVS IMWELDKASY EIKKVWYGRT IIRSAYKLFY EAAQELLDGN
610 620 630 640 650
LSVVDDIPEF KDLDEKSRQA KLEELVWAIG KLTDIARHVR AKRDGCGALE
660 670 680 690 700
LEGVEVCVQL DDKKNIHDLI PKQPLEVHET VAECMILANH WVAKKIWESF
710 720 730 740 750
PHQALLRQHP PPHQEFFSEL RECAKAKGFF IDTRSNKTLA DSLDNANDPH
760 770 780 790 800
DPIVNRLLRS MATQAMSNAL YFSTGSCAEE EFHHYGLALD KYTHFTSPIR
810 820 830 840 850
RYSDIVVHRL LMAAISKDKK MEIKGNLFSN KDLEELCRHI NNRNQAAQHS
860 870 880 890 900
QKQSTELFQC MYFKDKDPAT EERCISDGVI YSIRTNGVLL FIPRFGIKGA
910 920 930 940 950
AYLKNKDGLV ISCGPDSCSE WKPGSLQRFQ NKITSTTTDG ESVTFHLFDH
960 970 980 990 1000
VTVRISIQAS RCHSDTIRLE IISNKPYKIP NTELIHQSSP LLKSELVKEV
1010 1020 1030 1040 1050
TKSVEEAQLA QEVKVNIIQE EYQEYRQTKG RSLYTLLEEI RDLALLDVSN

NYGI
Length:1,054
Mass (Da):120,787
Last modified:January 15, 2008 - v2
Checksum:iB57B691CC25F65B8
GO
Isoform 2 (identifier: Q8TF46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: Missing.

Note: No experimental confirmation available.

Show »
Length:920
Mass (Da):105,012
Checksum:i2F7FB46F906C79A0
GO
Isoform 3 (identifier: Q8TF46-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     735-750: SNKTLADSLDNANDPH → YSSFEGAEEWSGMLYI
     751-1054: Missing.

Note: No experimental confirmation available.

Show »
Length:750
Mass (Da):86,260
Checksum:iDBACF862A569703A
GO
Isoform 4 (identifier: Q8TF46-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-83: Missing.

Note: No experimental confirmation available.

Show »
Length:971
Mass (Da):111,119
Checksum:iA3145DE249DF5279
GO

Sequence cautioni

The sequence BAB85541.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti518 – 5181I → V.
Corresponds to variant rs34668776 [ dbSNP | Ensembl ].
VAR_038056
Natural varianti614 – 6141D → G.2 Publications
Corresponds to variant rs3803412 [ dbSNP | Ensembl ].
VAR_038057
Natural varianti747 – 7471N → S.
Corresponds to variant rs17258507 [ dbSNP | Ensembl ].
VAR_038058

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 134134Missing in isoform 2. 1 PublicationVSP_030366Add
BLAST
Alternative sequencei1 – 8383Missing in isoform 4. 1 PublicationVSP_030367Add
BLAST
Alternative sequencei735 – 75016SNKTL…ANDPH → YSSFEGAEEWSGMLYI in isoform 3. 1 PublicationVSP_030368Add
BLAST
Alternative sequencei751 – 1054304Missing in isoform 3. 1 PublicationVSP_030369Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075835 mRNA. Translation: BAB85541.1. Different initiation.
AK095407 mRNA. Translation: BAC04542.1.
AC055855 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77759.1.
BC014124 mRNA. Translation: AAH14124.2.
BC022089 mRNA. Translation: AAH22089.1.
CCDSiCCDS10214.1. [Q8TF46-4]
CCDS45286.1. [Q8TF46-1]
RefSeqiNP_001137160.1. NM_001143688.1. [Q8TF46-1]
NP_588616.1. NM_133375.3. [Q8TF46-4]
XP_005254202.1. XM_005254145.2. [Q8TF46-4]
XP_005254204.1. XM_005254147.3. [Q8TF46-4]
UniGeneiHs.446251.
Hs.658492.

Genome annotation databases

EnsembliENST00000319194; ENSP00000321583; ENSG00000166938. [Q8TF46-4]
ENST00000319212; ENSP00000321711; ENSG00000166938. [Q8TF46-1]
GeneIDi115752.
KEGGihsa:115752.
UCSCiuc002app.3. human. [Q8TF46-1]
uc002apq.2. human. [Q8TF46-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075835 mRNA. Translation: BAB85541.1. Different initiation.
AK095407 mRNA. Translation: BAC04542.1.
AC055855 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77759.1.
BC014124 mRNA. Translation: AAH14124.2.
BC022089 mRNA. Translation: AAH22089.1.
CCDSiCCDS10214.1. [Q8TF46-4]
CCDS45286.1. [Q8TF46-1]
RefSeqiNP_001137160.1. NM_001143688.1. [Q8TF46-1]
NP_588616.1. NM_133375.3. [Q8TF46-4]
XP_005254202.1. XM_005254145.2. [Q8TF46-4]
XP_005254204.1. XM_005254147.3. [Q8TF46-4]
UniGeneiHs.446251.
Hs.658492.

3D structure databases

ProteinModelPortaliQ8TF46.
SMRiQ8TF46. Positions 9-955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125453. 21 interactions.
IntActiQ8TF46. 15 interactions.
STRINGi9606.ENSP00000321711.

PTM databases

PhosphoSiteiQ8TF46.

Polymorphism and mutation databases

BioMutaiDIS3L.
DMDMi166201903.

Proteomic databases

MaxQBiQ8TF46.
PaxDbiQ8TF46.
PeptideAtlasiQ8TF46.
PRIDEiQ8TF46.

Protocols and materials databases

DNASUi115752.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000319194; ENSP00000321583; ENSG00000166938. [Q8TF46-4]
ENST00000319212; ENSP00000321711; ENSG00000166938. [Q8TF46-1]
GeneIDi115752.
KEGGihsa:115752.
UCSCiuc002app.3. human. [Q8TF46-1]
uc002apq.2. human. [Q8TF46-3]

Organism-specific databases

CTDi115752.
GeneCardsiGC15P066585.
HGNCiHGNC:28698. DIS3L.
HPAiHPA041805.
MIMi614183. gene.
neXtProtiNX_Q8TF46.
PharmGKBiPA162383675.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0557.
GeneTreeiENSGT00530000063106.
HOGENOMiHOG000191945.
HOVERGENiHBG059397.
InParanoidiQ8TF46.
KOiK18681.
OMAiIPRFGIK.
OrthoDBiEOG77T13R.
PhylomeDBiQ8TF46.
TreeFamiTF105755.

Miscellaneous databases

ChiTaRSiDIS3L. human.
GeneWikiiDIS3L.
GenomeRNAii115752.
NextBioi79674.
PROiQ8TF46.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TF46.
CleanExiHS_DIS3L.
ExpressionAtlasiQ8TF46. baseline and differential.
GenevestigatoriQ8TF46.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR022966. RNase_II/R_CS.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 4 hits.
PROSITEiPS01175. RIBONUCLEASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT GLY-614.
    Tissue: Tongue.
  3. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), VARIANT GLY-614.
    Tissue: Kidney and Muscle.
  6. Cited for: ASSOCIATION WITH THE RNA EXOSOME COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, COFACTOR, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-486.
  7. "Dis3-like 1: a novel exoribonuclease associated with the human exosome."
    Staals R.H., Bronkhorst A.W., Schilders G., Slomovic S., Schuster G., Heck A.J., Raijmakers R., Pruijn G.J.
    EMBO J. 29:2358-2367(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-62; ASP-166 AND ASP-486.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDI3L1_HUMAN
AccessioniPrimary (citable) accession number: Q8TF46
Secondary accession number(s): Q8N1N8, Q8WTU9, Q96CM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: April 29, 2015
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.