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Protein

Ubiquitin-associated and SH3 domain-containing protein B

Gene

UBASH3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei390By similarity1
Active sitei391Tele-phosphohistidine intermediateBy similarity1
Active sitei576By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154127-MONOMER.
SignaLinkiQ8TF42.
SIGNORiQ8TF42.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)
Alternative name(s):
Cbl-interacting protein p70
Suppressor of T-cell receptor signaling 1
Short name:
STS-1
T-cell ubiquitin ligand 2
Short name:
TULA-2
Tyrosine-protein phosphatase STS1/TULA2
Gene namesi
Name:UBASH3B
Synonyms:KIAA1959, STS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:29884. UBASH3B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi84959.
OpenTargetsiENSG00000154127.
PharmGKBiPA162407840.

Polymorphism and mutation databases

BioMutaiUBASH3B.
DMDMi110287974.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455081 – 649Ubiquitin-associated and SH3 domain-containing protein BAdd BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineCombined sources1
Modified residuei23PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TF42.
MaxQBiQ8TF42.
PaxDbiQ8TF42.
PeptideAtlasiQ8TF42.
PRIDEiQ8TF42.

PTM databases

DEPODiQ8TF42.
iPTMnetiQ8TF42.
PhosphoSitePlusiQ8TF42.

Expressioni

Gene expression databases

BgeeiENSG00000154127.
CleanExiHS_UBASH3B.
GenevisibleiQ8TF42. HS.

Organism-specific databases

HPAiHPA038605.
HPA038607.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-1380492,EBI-1380492
CBLBQ131915EBI-1380492,EBI-744027
DAZAP2Q150385EBI-1380492,EBI-724310
DMRT3Q9NQL93EBI-1380492,EBI-9679045
FAM168AQ925673EBI-1380492,EBI-7957930
RCHY1Q96PM53EBI-1380492,EBI-947779
SLC25A48Q6ZT893EBI-1380492,EBI-10255185
SPRY2O435973EBI-1380492,EBI-742487
SYKP434052EBI-1380492,EBI-78302
VPS37BQ9H9H45EBI-1380492,EBI-4400866

Protein-protein interaction databases

BioGridi124390. 110 interactors.
IntActiQ8TF42. 47 interactors.
MINTiMINT-4829673.
STRINGi9606.ENSP00000284273.

Structurei

Secondary structure

1649
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Helixi40 – 47Combined sources8
Helixi51 – 60Combined sources10
Turni61 – 63Combined sources3
Helixi66 – 74Combined sources9
Beta strandi252 – 254Combined sources3
Beta strandi257 – 261Combined sources5
Beta strandi268 – 272Combined sources5
Beta strandi280 – 283Combined sources4
Helixi285 – 287Combined sources3
Turni291 – 294Combined sources4
Beta strandi295 – 303Combined sources9
Beta strandi306 – 310Combined sources5
Helixi311 – 313Combined sources3
Beta strandi314 – 316Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CPWNMR-A26-76[»]
2E5KNMR-A248-328[»]
ProteinModelPortaliQ8TF42.
SMRiQ8TF42.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TF42.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 76UBAPROSITE-ProRule annotationAdd BLAST50
Domaini254 – 319SH3PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 649Protein tyrosine phosphataseBy similarityAdd BLAST270

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8TF42.
KOiK18993.
OMAiFKAPDEC.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ8TF42.
TreeFamiTF313334.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TF42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQYGHPSPL GMAAREELYS KVTPRRNRQQ RPGTIKHGSA LDVLLSMGFP
60 70 80 90 100
RARAQKALAS TGGRSVQAAC DWLFSHVGDP FLDDPLPREY VLYLRPTGPL
110 120 130 140 150
AQKLSDFWQQ SKQICGKNKA HNIFPHITLC QFFMCEDSKV DALGEALQTT
160 170 180 190 200
VSRWKCKFSA PLPLELYTSS NFIGLFVKED SAEVLKKFAA DFAAEAASKT
210 220 230 240 250
EVHVEPHKKQ LHVTLAYHFQ ASHLPTLEKL AQNIDVKLGC DWVATIFSRD
260 270 280 290 300
IRFANHETLQ VIYPYTPQND DELELVPGDF IFMSPMEQTS TSEGWIYGTS
310 320 330 340 350
LTTGCSGLLP ENYITKADEC STWIFHGSYS ILNTSSSNSL TFGDGVLERR
360 370 380 390 400
PYEDQGLGET TPLTIICQPM QPLRVNSQPG PQKRCLFVCR HGERMDVVFG
410 420 430 440 450
KYWLSQCFDA KGRYIRTNLN MPHSLPQRSG GFRDYEKDAP ITVFGCMQAR
460 470 480 490 500
LVGEALLESN TIIDHVYCSP SLRCVQTAHN ILKGLQQENH LKIRVEPGLF
510 520 530 540 550
EWTKWVAGST LPAWIPPSEL AAANLSVDTT YRPHIPISKL VVSESYDTYI
560 570 580 590 600
SRSFQVTKEI ISECKSKGNN ILIVAHASSL EACTCQLQGL SPQNSKDFVQ
610 620 630 640
MVRKIPYLGF CSCEELGETG IWQLTDPPIL PLTHGPTGGF NWRETLLQE
Length:649
Mass (Da):72,696
Last modified:July 11, 2006 - v2
Checksum:i70ED9F50EA20BF43
GO

Sequence cautioni

The sequence AAL16953 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB85545 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti465H → R in BAD96563 (Ref. 3) Curated1
Sequence conflicti465H → R in BAD96566 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05267668A → T.1 PublicationCorresponds to variant rs12790613dbSNPEnsembl.1
Natural variantiVAR_061923569N → S.Corresponds to variant rs35343548dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075839 mRNA. Translation: BAB85545.1. Different initiation.
AK075203 mRNA. Translation: BAC11468.1.
AK222843 mRNA. Translation: BAD96563.1.
AK222846 mRNA. Translation: BAD96566.1.
BC007541 mRNA. Translation: AAH07541.2.
AF425252 mRNA. Translation: AAL16953.1. Different initiation.
CCDSiCCDS31694.1.
RefSeqiNP_116262.2. NM_032873.4.
UniGeneiHs.444075.

Genome annotation databases

EnsembliENST00000284273; ENSP00000284273; ENSG00000154127.
GeneIDi84959.
KEGGihsa:84959.
UCSCiuc001pyi.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075839 mRNA. Translation: BAB85545.1. Different initiation.
AK075203 mRNA. Translation: BAC11468.1.
AK222843 mRNA. Translation: BAD96563.1.
AK222846 mRNA. Translation: BAD96566.1.
BC007541 mRNA. Translation: AAH07541.2.
AF425252 mRNA. Translation: AAL16953.1. Different initiation.
CCDSiCCDS31694.1.
RefSeqiNP_116262.2. NM_032873.4.
UniGeneiHs.444075.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CPWNMR-A26-76[»]
2E5KNMR-A248-328[»]
ProteinModelPortaliQ8TF42.
SMRiQ8TF42.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124390. 110 interactors.
IntActiQ8TF42. 47 interactors.
MINTiMINT-4829673.
STRINGi9606.ENSP00000284273.

PTM databases

DEPODiQ8TF42.
iPTMnetiQ8TF42.
PhosphoSitePlusiQ8TF42.

Polymorphism and mutation databases

BioMutaiUBASH3B.
DMDMi110287974.

Proteomic databases

EPDiQ8TF42.
MaxQBiQ8TF42.
PaxDbiQ8TF42.
PeptideAtlasiQ8TF42.
PRIDEiQ8TF42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284273; ENSP00000284273; ENSG00000154127.
GeneIDi84959.
KEGGihsa:84959.
UCSCiuc001pyi.5. human.

Organism-specific databases

CTDi84959.
DisGeNETi84959.
GeneCardsiUBASH3B.
HGNCiHGNC:29884. UBASH3B.
HPAiHPA038605.
HPA038607.
MIMi609201. gene.
neXtProtiNX_Q8TF42.
OpenTargetsiENSG00000154127.
PharmGKBiPA162407840.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8TF42.
KOiK18993.
OMAiFKAPDEC.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ8TF42.
TreeFamiTF313334.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000154127-MONOMER.
SignaLinkiQ8TF42.
SIGNORiQ8TF42.

Miscellaneous databases

ChiTaRSiUBASH3B. human.
EvolutionaryTraceiQ8TF42.
GeneWikiiSTS-1_(gene).
GenomeRNAii84959.
PROiQ8TF42.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154127.
CleanExiHS_UBASH3B.
GenevisibleiQ8TF42. HS.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBS3B_HUMAN
AccessioniPrimary (citable) accession number: Q8TF42
Secondary accession number(s): Q53GT5
, Q53GT8, Q8NBV7, Q96IG9, Q96NZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.