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Q8TF42

- UBS3B_HUMAN

UniProt

Q8TF42 - UBS3B_HUMAN

Protein

Ubiquitin-associated and SH3 domain-containing protein B

Gene

UBASH3B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.2 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei390 – 3901By similarity
    Active sitei391 – 3911Tele-phosphohistidine intermediateBy similarity
    Active sitei576 – 5761By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. negative regulation of platelet aggregation Source: Ensembl
    2. negative regulation of protein kinase activity Source: Ensembl
    3. regulation of release of sequestered calcium ion into cytosol Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    SignaLinkiQ8TF42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)
    Alternative name(s):
    Cbl-interacting protein p70
    Suppressor of T-cell receptor signaling 1
    Short name:
    STS-1
    T-cell ubiquitin ligand 2
    Short name:
    TULA-2
    Tyrosine-protein phosphatase STS1/TULA2
    Gene namesi
    Name:UBASH3B
    Synonyms:KIAA1959, STS1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:29884. UBASH3B.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162407840.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 649649Ubiquitin-associated and SH3 domain-containing protein BPRO_0000245508Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201Phosphoserine1 Publication
    Modified residuei23 – 231Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8TF42.
    PaxDbiQ8TF42.
    PRIDEiQ8TF42.

    PTM databases

    PhosphoSiteiQ8TF42.

    Expressioni

    Gene expression databases

    BgeeiQ8TF42.
    CleanExiHS_UBASH3B.
    GenevestigatoriQ8TF42.

    Organism-specific databases

    HPAiHPA038605.
    HPA038607.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SYKP434052EBI-1380492,EBI-78302

    Protein-protein interaction databases

    BioGridi124390. 74 interactions.
    IntActiQ8TF42. 24 interactions.
    MINTiMINT-4829673.
    STRINGi9606.ENSP00000284273.

    Structurei

    Secondary structure

    1
    649
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Helixi40 – 478
    Helixi51 – 6010
    Turni61 – 633
    Helixi66 – 749
    Beta strandi252 – 2543
    Beta strandi257 – 2615
    Beta strandi268 – 2725
    Beta strandi280 – 2834
    Helixi285 – 2873
    Turni291 – 2944
    Beta strandi295 – 3039
    Beta strandi306 – 3105
    Helixi311 – 3133
    Beta strandi314 – 3163

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CPWNMR-A26-76[»]
    2E5KNMR-A248-328[»]
    ProteinModelPortaliQ8TF42.
    SMRiQ8TF42. Positions 26-78, 249-328, 384-647.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8TF42.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 7650UBAPROSITE-ProRule annotationAdd
    BLAST
    Domaini254 – 31966SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni380 – 649270Protein tyrosine phosphataseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SH3 domain.PROSITE-ProRule annotation
    Contains 1 UBA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG276661.
    HOGENOMiHOG000012936.
    HOVERGENiHBG018025.
    InParanoidiQ8TF42.
    OMAiADECGTW.
    OrthoDBiEOG7P5T0D.
    PhylomeDBiQ8TF42.
    TreeFamiTF313334.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR001452. SH3_domain.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    [Graphical view]
    PfamiPF00300. His_Phos_1. 1 hit.
    PF14604. SH3_9. 1 hit.
    PF00627. UBA. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF53254. SSF53254. 2 hits.
    PROSITEiPS50002. SH3. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8TF42-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQYGHPSPL GMAAREELYS KVTPRRNRQQ RPGTIKHGSA LDVLLSMGFP    50
    RARAQKALAS TGGRSVQAAC DWLFSHVGDP FLDDPLPREY VLYLRPTGPL 100
    AQKLSDFWQQ SKQICGKNKA HNIFPHITLC QFFMCEDSKV DALGEALQTT 150
    VSRWKCKFSA PLPLELYTSS NFIGLFVKED SAEVLKKFAA DFAAEAASKT 200
    EVHVEPHKKQ LHVTLAYHFQ ASHLPTLEKL AQNIDVKLGC DWVATIFSRD 250
    IRFANHETLQ VIYPYTPQND DELELVPGDF IFMSPMEQTS TSEGWIYGTS 300
    LTTGCSGLLP ENYITKADEC STWIFHGSYS ILNTSSSNSL TFGDGVLERR 350
    PYEDQGLGET TPLTIICQPM QPLRVNSQPG PQKRCLFVCR HGERMDVVFG 400
    KYWLSQCFDA KGRYIRTNLN MPHSLPQRSG GFRDYEKDAP ITVFGCMQAR 450
    LVGEALLESN TIIDHVYCSP SLRCVQTAHN ILKGLQQENH LKIRVEPGLF 500
    EWTKWVAGST LPAWIPPSEL AAANLSVDTT YRPHIPISKL VVSESYDTYI 550
    SRSFQVTKEI ISECKSKGNN ILIVAHASSL EACTCQLQGL SPQNSKDFVQ 600
    MVRKIPYLGF CSCEELGETG IWQLTDPPIL PLTHGPTGGF NWRETLLQE 649
    Length:649
    Mass (Da):72,696
    Last modified:July 11, 2006 - v2
    Checksum:i70ED9F50EA20BF43
    GO

    Sequence cautioni

    The sequence AAL16953.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB85545.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti465 – 4651H → R in BAD96563. 1 PublicationCurated
    Sequence conflicti465 – 4651H → R in BAD96566. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti68 – 681A → T.1 Publication
    Corresponds to variant rs12790613 [ dbSNP | Ensembl ].
    VAR_052676
    Natural varianti569 – 5691N → S.
    Corresponds to variant rs35343548 [ dbSNP | Ensembl ].
    VAR_061923

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB075839 mRNA. Translation: BAB85545.1. Different initiation.
    AK075203 mRNA. Translation: BAC11468.1.
    AK222843 mRNA. Translation: BAD96563.1.
    AK222846 mRNA. Translation: BAD96566.1.
    BC007541 mRNA. Translation: AAH07541.2.
    AF425252 mRNA. Translation: AAL16953.1. Different initiation.
    CCDSiCCDS31694.1.
    RefSeqiNP_116262.2. NM_032873.4.
    UniGeneiHs.444075.

    Genome annotation databases

    EnsembliENST00000284273; ENSP00000284273; ENSG00000154127.
    GeneIDi84959.
    KEGGihsa:84959.
    UCSCiuc001pyi.4. human.

    Polymorphism databases

    DMDMi110287974.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB075839 mRNA. Translation: BAB85545.1 . Different initiation.
    AK075203 mRNA. Translation: BAC11468.1 .
    AK222843 mRNA. Translation: BAD96563.1 .
    AK222846 mRNA. Translation: BAD96566.1 .
    BC007541 mRNA. Translation: AAH07541.2 .
    AF425252 mRNA. Translation: AAL16953.1 . Different initiation.
    CCDSi CCDS31694.1.
    RefSeqi NP_116262.2. NM_032873.4.
    UniGenei Hs.444075.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CPW NMR - A 26-76 [» ]
    2E5K NMR - A 248-328 [» ]
    ProteinModelPortali Q8TF42.
    SMRi Q8TF42. Positions 26-78, 249-328, 384-647.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124390. 74 interactions.
    IntActi Q8TF42. 24 interactions.
    MINTi MINT-4829673.
    STRINGi 9606.ENSP00000284273.

    PTM databases

    PhosphoSitei Q8TF42.

    Polymorphism databases

    DMDMi 110287974.

    Proteomic databases

    MaxQBi Q8TF42.
    PaxDbi Q8TF42.
    PRIDEi Q8TF42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000284273 ; ENSP00000284273 ; ENSG00000154127 .
    GeneIDi 84959.
    KEGGi hsa:84959.
    UCSCi uc001pyi.4. human.

    Organism-specific databases

    CTDi 84959.
    GeneCardsi GC11P122526.
    HGNCi HGNC:29884. UBASH3B.
    HPAi HPA038605.
    HPA038607.
    MIMi 609201. gene.
    neXtProti NX_Q8TF42.
    PharmGKBi PA162407840.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG276661.
    HOGENOMi HOG000012936.
    HOVERGENi HBG018025.
    InParanoidi Q8TF42.
    OMAi ADECGTW.
    OrthoDBi EOG7P5T0D.
    PhylomeDBi Q8TF42.
    TreeFami TF313334.

    Enzyme and pathway databases

    SignaLinki Q8TF42.

    Miscellaneous databases

    EvolutionaryTracei Q8TF42.
    GeneWikii STS-1_(gene).
    GenomeRNAii 84959.
    NextBioi 75468.
    PROi Q8TF42.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8TF42.
    CleanExi HS_UBASH3B.
    Genevestigatori Q8TF42.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    IPR001452. SH3_domain.
    IPR009060. UBA-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    IPR000449. UBA/Ts_N.
    [Graphical view ]
    Pfami PF00300. His_Phos_1. 1 hit.
    PF14604. SH3_9. 1 hit.
    PF00627. UBA. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    SM00165. UBA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF53254. SSF53254. 2 hits.
    PROSITEi PS50002. SH3. 1 hit.
    PS50030. UBA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
      Nagase T., Kikuno R., Ohara O.
      DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
      Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
      , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
      DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    3. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-68.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. Bienvenut W.V., Claeys D.
      Submitted (NOV-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 89-112; 188-199; 230-237 AND 417-428, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Platelet.
    6. "A novel SH3 containing protein is phosphorylated by the nm23-H1 protein kinase activity in vitro and shows reduced mRNA expression in human tumors."
      Engel M., Seifert M., Maschlanka M., Welter C.
      Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 116-649.
    7. "Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases."
      Kowanetz K., Crosetto N., Haglund K., Schmidt M.H., Heldin C.-H., Dikic I.
      J. Biol. Chem. 279:32786-32795(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CBL AND UBIQUITIN, IDENTIFICATION IN A COMPLEX WITH EGFR.
    8. "Suppressor of T-cell receptor signalling 1 and 2 differentially regulate endocytosis and signalling of receptor tyrosine kinases."
      Raguz J., Wagner S., Dikic I., Hoeller D.
      FEBS Lett. 581:4767-4772(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND THR-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "Solution structure of RSGI RUH-031, a UBA domain from human cDNA."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2005) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 26-76.
    11. "Solution structure of SH3 domain in suppressor of T-cell receptor signaling 1."
      RIKEN structural genomics initiative (RSGI)
      Submitted (JUN-2007) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 248-328.

    Entry informationi

    Entry nameiUBS3B_HUMAN
    AccessioniPrimary (citable) accession number: Q8TF42
    Secondary accession number(s): Q53GT5
    , Q53GT8, Q8NBV7, Q96IG9, Q96NZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3