Q8TF42 (UBS3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-associated and SH3 domain-containing protein B
EC=3.1.3.48

Alternative name(s):
Cbl-interacting protein p70
Suppressor of T-cell receptor signaling 1
Short name=STS-1
T-cell ubiquitin ligand 2
Short name=TULA-2
Tyrosine-protein phosphatase STS1/TULA2

Gene names

Name:	UBASH3B
Synonyms:	KIAA1959, STS1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination. Ref.7 Ref.8
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Ref.8
Subunit structure	Homodimer By similarity. Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Ref.7
Subcellular location	Cytoplasm By similarity. Nucleus Potential.
Sequence similarities	Contains 1 SH3 domain. Contains 1 UBA domain.
Sequence caution	The sequence AAL16953.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB85545.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	SH3 domain
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 649

649

Ubiquitin-associated and SH3 domain-containing protein B

PRO_0000245508

Regions

Domain

27 – 76

50

UBA

Domain

254 – 319

66

SH3

Region

380 – 649

270

Protein tyrosine phosphatase By similarity

Sites

Active site

390

1

By similarity

Active site

391

1

Tele-phosphohistidine intermediate By similarity

Active site

576

1

By similarity

Amino acid modifications

Modified residue

19

1

Phosphotyrosine Ref.10

Modified residue

20

1

Phosphoserine Ref.9

Modified residue

23

1

Phosphothreonine Ref.9

Natural variations

Natural variant

68

1

A → T. Ref.3
Corresponds to variant rs12790613 [ dbSNP | Ensembl ].

VAR_052676

Natural variant

569

1

N → S.
Corresponds to variant rs35343548 [ dbSNP | Ensembl ].

VAR_061923

Experimental info

Sequence conflict

465

1

H → R in BAD96563. Ref.3

Sequence conflict

465

1

H → R in BAD96566. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

649

Helix Strand Turn

Details...

Beta strand

252 – 254

3

Beta strand

257 – 261

5

Beta strand

268 – 272

5

Beta strand

280 – 283

4

Helix

285 – 287

3

Turn

291 – 294

4

Beta strand

295 – 303

9

Beta strand

306 – 310

5

Helix

311 – 313

3

Beta strand

314 – 316

3

Sequences

	Sequence		Length	Mass (Da)
	Q8TF42 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: 70ED9F50EA20BF43	FASTA	649	72,696
	10 20 30 40 50 60 MAQYGHPSPL GMAAREELYS KVTPRRNRQQ RPGTIKHGSA LDVLLSMGFP RARAQKALAS 70 80 90 100 110 120 TGGRSVQAAC DWLFSHVGDP FLDDPLPREY VLYLRPTGPL AQKLSDFWQQ SKQICGKNKA 130 140 150 160 170 180 HNIFPHITLC QFFMCEDSKV DALGEALQTT VSRWKCKFSA PLPLELYTSS NFIGLFVKED 190 200 210 220 230 240 SAEVLKKFAA DFAAEAASKT EVHVEPHKKQ LHVTLAYHFQ ASHLPTLEKL AQNIDVKLGC 250 260 270 280 290 300 DWVATIFSRD IRFANHETLQ VIYPYTPQND DELELVPGDF IFMSPMEQTS TSEGWIYGTS 310 320 330 340 350 360 LTTGCSGLLP ENYITKADEC STWIFHGSYS ILNTSSSNSL TFGDGVLERR PYEDQGLGET 370 380 390 400 410 420 TPLTIICQPM QPLRVNSQPG PQKRCLFVCR HGERMDVVFG KYWLSQCFDA KGRYIRTNLN 430 440 450 460 470 480 MPHSLPQRSG GFRDYEKDAP ITVFGCMQAR LVGEALLESN TIIDHVYCSP SLRCVQTAHN 490 500 510 520 530 540 ILKGLQQENH LKIRVEPGLF EWTKWVAGST LPAWIPPSEL AAANLSVDTT YRPHIPISKL 550 560 570 580 590 600 VVSESYDTYI SRSFQVTKEI ISECKSKGNN ILIVAHASSL EACTCQLQGL SPQNSKDFVQ 610 620 630 640 MVRKIPYLGF CSCEELGETG IWQLTDPPIL PLTHGPTGGF NWRETLLQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:319-327(2001) [PubMed: 11853319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-68. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 89-112; 188-199; 230-237 AND 417-428, MASS SPECTROMETRY. Tissue: Platelet.
[6]	"A novel SH3 containing protein is phosphorylated by the nm23-H1 protein kinase activity in vitro and shows reduced mRNA expression in human tumors." Engel M., Seifert M., Maschlanka M., Welter C. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 116-649.
[7]	"Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases." Kowanetz K., Crosetto N., Haglund K., Schmidt M.H., Heldin C.-H., Dikic I. J. Biol. Chem. 279:32786-32795(2004) [PubMed: 15159412] [Abstract] Cited for: FUNCTION, INTERACTION WITH CBL AND UBIQUITIN, IDENTIFICATION IN A COMPLEX WITH EGFR.
[8]	"Suppressor of T-cell receptor signalling 1 and 2 differentially regulate endocytosis and signalling of receptor tyrosine kinases." Raguz J., Wagner S., Dikic I., Hoeller D. FEBS Lett. 581:4767-4772(2007) [PubMed: 17880946] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND THR-23, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-19, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[11]	"Solution structure of RSGI RUH-031, a UBA domain from human cDNA." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 26-76.
[12]	"Solution structure of SH3 domain in suppressor of T-cell receptor signaling 1." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 248-328.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB075839 mRNA. Translation: BAB85545.1. Different initiation.
AK075203 mRNA. Translation: BAC11468.1.
AK222843 mRNA. Translation: BAD96563.1.
AK222846 mRNA. Translation: BAD96566.1.
BC007541 mRNA. Translation: AAH07541.2.
AF425252 mRNA. Translation: AAL16953.1. Different initiation.

IPI

IPI00154910.

RefSeq

NP_116262.2. NM_032873.4.

UniGene

Hs.444075.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CPW	NMR	-	A	26-76	[»]
2E5K	NMR	-	A	248-328	[»]

ProteinModelPortal

Q8TF42.

SMR

Q8TF42. Positions 26-78, 249-328, 384-647.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8TF42. 11 interactions.

MINT

MINT-4829673.

STRING

Q8TF42.

PTM databases

PhosphoSite

Q8TF42.

Polymorphism databases

DMDM

110287974.

Proteomic databases

PRIDE

Q8TF42.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000284273; ENSP00000284273; ENSG00000154127.

GeneID

84959.

KEGG

hsa:84959.

UCSC

uc001pyi.2. human.

Organism-specific databases

CTD

84959.

GeneCards

GC11P122526.

H-InvDB

HIX0010211.

HGNC

HGNC:29884. UBASH3B.

HPA

HPA038605.

MIM

609201. gene.

neXtProt

NX_Q8TF42.

PharmGKB

PA162407840.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG04022.

GeneTree

ENSGT00390000018249.

HOGENOM

HBG402867.

HOVERGEN

HBG018025.

InParanoid

Q8TF42.

OMA

LERRPYE.

OrthoDB

EOG4NKBV1.

PhylomeDB

Q8TF42.

Gene expression databases

ArrayExpress

Q8TF42.

Bgee

Q8TF42.

CleanEx

HS_UBASH3B.

Genevestigator

Q8TF42.

Family and domain databases

InterPro

IPR013078. His_Pase_superF_clade-1.
IPR001452. SH3_domain.
IPR009060. UBA-like.
IPR000449. UBA/transl_elong_EF1B_N.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]

Pfam

PF00300. His_Phos_1. 1 hit.
PF00018. SH3_1. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]

SMART

SM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.
SSF46934. UBA_like. 1 hit.

PROSITE

PS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

75468.

SOURCE

Search...

Entry information

Entry name

UBS3B_HUMAN

Accession

Primary (citable) accession number: Q8TF42
Secondary accession number(s): Q53GT5 , Q53GT8, Q8NBV7, Q96IG9, Q96NZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 11, 2006
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.