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Protein

Ubiquitin-associated and SH3 domain-containing protein B

Gene

UBASH3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei390 – 3901By similarity
Active sitei391 – 3911Tele-phosphohistidine intermediateBy similarity
Active sitei576 – 5761By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of platelet aggregation Source: Ensembl
  2. negative regulation of protein kinase activity Source: Ensembl
  3. regulation of release of sequestered calcium ion into cytosol Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

SignaLinkiQ8TF42.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)
Alternative name(s):
Cbl-interacting protein p70
Suppressor of T-cell receptor signaling 1
Short name:
STS-1
T-cell ubiquitin ligand 2
Short name:
TULA-2
Tyrosine-protein phosphatase STS1/TULA2
Gene namesi
Name:UBASH3B
Synonyms:KIAA1959, STS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:29884. UBASH3B.

Subcellular locationi

Cytoplasm By similarity. Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162407840.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Ubiquitin-associated and SH3 domain-containing protein BPRO_0000245508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Phosphoserine1 Publication
Modified residuei23 – 231Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8TF42.
PaxDbiQ8TF42.
PRIDEiQ8TF42.

PTM databases

DEPODiQ8TF42.
PhosphoSiteiQ8TF42.

Expressioni

Gene expression databases

BgeeiQ8TF42.
CleanExiHS_UBASH3B.
GenevestigatoriQ8TF42.

Organism-specific databases

HPAiHPA038605.
HPA038607.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SYKP434052EBI-1380492,EBI-78302

Protein-protein interaction databases

BioGridi124390. 85 interactions.
IntActiQ8TF42. 25 interactions.
MINTiMINT-4829673.
STRINGi9606.ENSP00000284273.

Structurei

Secondary structure

1
649
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 363Combined sources
Helixi40 – 478Combined sources
Helixi51 – 6010Combined sources
Turni61 – 633Combined sources
Helixi66 – 749Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi257 – 2615Combined sources
Beta strandi268 – 2725Combined sources
Beta strandi280 – 2834Combined sources
Helixi285 – 2873Combined sources
Turni291 – 2944Combined sources
Beta strandi295 – 3039Combined sources
Beta strandi306 – 3105Combined sources
Helixi311 – 3133Combined sources
Beta strandi314 – 3163Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CPWNMR-A26-76[»]
2E5KNMR-A248-328[»]
ProteinModelPortaliQ8TF42.
SMRiQ8TF42. Positions 26-78, 249-328, 384-647.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TF42.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 7650UBAPROSITE-ProRule annotationAdd
BLAST
Domaini254 – 31966SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni380 – 649270Protein tyrosine phosphataseBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG276661.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8TF42.
OMAiDCPITVF.
OrthoDBiEOG7P5T0D.
PhylomeDBiQ8TF42.
TreeFamiTF313334.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001452. SH3_domain.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TF42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQYGHPSPL GMAAREELYS KVTPRRNRQQ RPGTIKHGSA LDVLLSMGFP
60 70 80 90 100
RARAQKALAS TGGRSVQAAC DWLFSHVGDP FLDDPLPREY VLYLRPTGPL
110 120 130 140 150
AQKLSDFWQQ SKQICGKNKA HNIFPHITLC QFFMCEDSKV DALGEALQTT
160 170 180 190 200
VSRWKCKFSA PLPLELYTSS NFIGLFVKED SAEVLKKFAA DFAAEAASKT
210 220 230 240 250
EVHVEPHKKQ LHVTLAYHFQ ASHLPTLEKL AQNIDVKLGC DWVATIFSRD
260 270 280 290 300
IRFANHETLQ VIYPYTPQND DELELVPGDF IFMSPMEQTS TSEGWIYGTS
310 320 330 340 350
LTTGCSGLLP ENYITKADEC STWIFHGSYS ILNTSSSNSL TFGDGVLERR
360 370 380 390 400
PYEDQGLGET TPLTIICQPM QPLRVNSQPG PQKRCLFVCR HGERMDVVFG
410 420 430 440 450
KYWLSQCFDA KGRYIRTNLN MPHSLPQRSG GFRDYEKDAP ITVFGCMQAR
460 470 480 490 500
LVGEALLESN TIIDHVYCSP SLRCVQTAHN ILKGLQQENH LKIRVEPGLF
510 520 530 540 550
EWTKWVAGST LPAWIPPSEL AAANLSVDTT YRPHIPISKL VVSESYDTYI
560 570 580 590 600
SRSFQVTKEI ISECKSKGNN ILIVAHASSL EACTCQLQGL SPQNSKDFVQ
610 620 630 640
MVRKIPYLGF CSCEELGETG IWQLTDPPIL PLTHGPTGGF NWRETLLQE
Length:649
Mass (Da):72,696
Last modified:July 11, 2006 - v2
Checksum:i70ED9F50EA20BF43
GO

Sequence cautioni

The sequence AAL16953.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB85545.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti465 – 4651H → R in BAD96563 (Ref. 3) Curated
Sequence conflicti465 – 4651H → R in BAD96566 (Ref. 3) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681A → T.1 Publication
Corresponds to variant rs12790613 [ dbSNP | Ensembl ].
VAR_052676
Natural varianti569 – 5691N → S.
Corresponds to variant rs35343548 [ dbSNP | Ensembl ].
VAR_061923

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075839 mRNA. Translation: BAB85545.1. Different initiation.
AK075203 mRNA. Translation: BAC11468.1.
AK222843 mRNA. Translation: BAD96563.1.
AK222846 mRNA. Translation: BAD96566.1.
BC007541 mRNA. Translation: AAH07541.2.
AF425252 mRNA. Translation: AAL16953.1. Different initiation.
CCDSiCCDS31694.1.
RefSeqiNP_116262.2. NM_032873.4.
UniGeneiHs.444075.

Genome annotation databases

EnsembliENST00000284273; ENSP00000284273; ENSG00000154127.
GeneIDi84959.
KEGGihsa:84959.
UCSCiuc001pyi.4. human.

Polymorphism databases

DMDMi110287974.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075839 mRNA. Translation: BAB85545.1. Different initiation.
AK075203 mRNA. Translation: BAC11468.1.
AK222843 mRNA. Translation: BAD96563.1.
AK222846 mRNA. Translation: BAD96566.1.
BC007541 mRNA. Translation: AAH07541.2.
AF425252 mRNA. Translation: AAL16953.1. Different initiation.
CCDSiCCDS31694.1.
RefSeqiNP_116262.2. NM_032873.4.
UniGeneiHs.444075.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CPWNMR-A26-76[»]
2E5KNMR-A248-328[»]
ProteinModelPortaliQ8TF42.
SMRiQ8TF42. Positions 26-78, 249-328, 384-647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124390. 85 interactions.
IntActiQ8TF42. 25 interactions.
MINTiMINT-4829673.
STRINGi9606.ENSP00000284273.

PTM databases

DEPODiQ8TF42.
PhosphoSiteiQ8TF42.

Polymorphism databases

DMDMi110287974.

Proteomic databases

MaxQBiQ8TF42.
PaxDbiQ8TF42.
PRIDEiQ8TF42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284273; ENSP00000284273; ENSG00000154127.
GeneIDi84959.
KEGGihsa:84959.
UCSCiuc001pyi.4. human.

Organism-specific databases

CTDi84959.
GeneCardsiGC11P122526.
HGNCiHGNC:29884. UBASH3B.
HPAiHPA038605.
HPA038607.
MIMi609201. gene.
neXtProtiNX_Q8TF42.
PharmGKBiPA162407840.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG276661.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ8TF42.
OMAiDCPITVF.
OrthoDBiEOG7P5T0D.
PhylomeDBiQ8TF42.
TreeFamiTF313334.

Enzyme and pathway databases

SignaLinkiQ8TF42.

Miscellaneous databases

ChiTaRSiUBASH3B. human.
EvolutionaryTraceiQ8TF42.
GeneWikiiSTS-1_(gene).
GenomeRNAii84959.
NextBioi75468.
PROiQ8TF42.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TF42.
CleanExiHS_UBASH3B.
GenevestigatoriQ8TF42.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR001452. SH3_domain.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins."
    Nagase T., Kikuno R., Ohara O.
    DNA Res. 8:319-327(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  3. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-68.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. Bienvenut W.V., Claeys D.
    Submitted (NOV-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 89-112; 188-199; 230-237 AND 417-428, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Platelet.
  6. "A novel SH3 containing protein is phosphorylated by the nm23-H1 protein kinase activity in vitro and shows reduced mRNA expression in human tumors."
    Engel M., Seifert M., Maschlanka M., Welter C.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 116-649.
  7. "Suppressors of T-cell receptor signaling Sts-1 and Sts-2 bind to Cbl and inhibit endocytosis of receptor tyrosine kinases."
    Kowanetz K., Crosetto N., Haglund K., Schmidt M.H., Heldin C.-H., Dikic I.
    J. Biol. Chem. 279:32786-32795(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CBL AND UBIQUITIN, IDENTIFICATION IN A COMPLEX WITH EGFR.
  8. "Suppressor of T-cell receptor signalling 1 and 2 differentially regulate endocytosis and signalling of receptor tyrosine kinases."
    Raguz J., Wagner S., Dikic I., Hoeller D.
    FEBS Lett. 581:4767-4772(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20 AND THR-23, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Solution structure of RSGI RUH-031, a UBA domain from human cDNA."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 26-76.
  11. "Solution structure of SH3 domain in suppressor of T-cell receptor signaling 1."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 248-328.

Entry informationi

Entry nameiUBS3B_HUMAN
AccessioniPrimary (citable) accession number: Q8TF42
Secondary accession number(s): Q53GT5
, Q53GT8, Q8NBV7, Q96IG9, Q96NZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: February 4, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.