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Protein

WASP homolog-associated protein with actin, membranes and microtubules

Gene

WHAMM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a nucleation-promoting factor (NPF) that stimulates Arp2/3-mediated actin polymerization both at the Golgi apparatus and along tubular membranes. Its activity in membrane tubulation requires F-actin and interaction with microtubules. Proposed to use coordinated actin-nucleating and microtubule-binding activities of distinct WHAMM molecules to drive membrane tubule elongation; when MT-bound can recruit and remodel membrane vesicles but is prevented to activate the Arp2/3 complex. Involved as a regulator of Golgi positioning and morphology. Participates in vesicle transport between the reticulum endoplasmic and the Golgi complex. Required for RhoD-dependent actin reorganization such as in cell adhesion and cell migration.3 Publications

GO - Molecular functioni

  • Arp2/3 complex binding Source: UniProtKB
  • GTP-Rho binding Source: UniProtKB
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • actin filament organization Source: UniProtKB
  • actin filament reorganization Source: UniProtKB
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • focal adhesion assembly Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • membrane tubulation Source: UniProtKB
  • positive regulation of actin nucleation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
WASP homolog-associated protein with actin, membranes and microtubules
Alternative name(s):
WAS protein homology region 2 domain-containing protein 1
Short name:
WH2 domain-containing protein 1
Gene namesi
Name:WHAMM
Synonyms:KIAA1971, WHDC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:30493. WHAMM.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB
  • Golgi membrane Source: UniProtKB
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi807W → A: Decreases nucleation-promoting factor activity and Arp2/3 complex activation. 1 Publication1

Organism-specific databases

DisGeNETi123720.
OpenTargetsiENSG00000156232.
PharmGKBiPA164727567.

Polymorphism and mutation databases

BioMutaiWHAMM.
DMDMi152112352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951001 – 809WASP homolog-associated protein with actin, membranes and microtubulesAdd BLAST809

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei606PhosphoserineCombined sources1
Modified residuei795PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TF30.
MaxQBiQ8TF30.
PaxDbiQ8TF30.
PeptideAtlasiQ8TF30.
PRIDEiQ8TF30.

PTM databases

iPTMnetiQ8TF30.
PhosphoSitePlusiQ8TF30.

Expressioni

Tissue specificityi

Expressed in brain, lung, heart, colon and kidney (at protein level).

Gene expression databases

BgeeiENSG00000156232.
CleanExiHS_WHDC1.
GenevisibleiQ8TF30. HS.

Organism-specific databases

HPAiHPA039690.
HPA040231.

Interactioni

Subunit structurei

Interacts with ACTR3; indicative for an association with the ARP2/3 complex. Associates with microtubules; in vitro binds to tubulin heterodimer in a 1:1 stoichiometry; decorates microtubules with a repeat of 80 A along protofilaments. Interacts with RHOD (in GTP-bound form).3 Publications

GO - Molecular functioni

  • Arp2/3 complex binding Source: UniProtKB
  • GTP-Rho binding Source: UniProtKB
  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi125830. 1 interactor.
STRINGi9606.ENSP00000286760.

Structurei

3D structure databases

ProteinModelPortaliQ8TF30.
SMRiQ8TF30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini709 – 727WH2 1PROSITE-ProRule annotationAdd BLAST19
Domaini739 – 756WH2 2PROSITE-ProRule annotationAdd BLAST18

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 260Mediates association with membranes1 PublicationAdd BLAST260
Regioni261 – 630Mediates interaction with microtubules1 PublicationAdd BLAST370
Regioni631 – 809Mediates actin nucleation1 PublicationAdd BLAST179

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili271 – 298Sequence analysisAdd BLAST28
Coiled coili384 – 479Sequence analysisAdd BLAST96
Coiled coili512 – 542Sequence analysisAdd BLAST31
Coiled coili770 – 797Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi639 – 658Pro-richAdd BLAST20

Domaini

The N-terminal region associates with membranes, the coiled-coil region binds to microtubules and the WH2 domains promotes actin nucleation.

Sequence similaritiesi

Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IF4S. Eukaryota.
ENOG4111J4B. LUCA.
GeneTreeiENSGT00510000046704.
HOGENOMiHOG000074181.
HOVERGENiHBG067146.
InParanoidiQ8TF30.
KOiK20479.
OMAiCGQLERY.
OrthoDBiEOG091G0217.
PhylomeDBiQ8TF30.
TreeFamiTF331023.

Family and domain databases

InterProiIPR031738. JMY/WHAMM.
IPR031808. JMY/WHAMM_N.
IPR003124. WH2_dom.
IPR026688. WHAMM.
[Graphical view]
PANTHERiPTHR23330:SF6. PTHR23330:SF6. 1 hit.
PfamiPF15871. JMY. 1 hit.
PF15920. WHAMM-JMY_N. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8TF30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDEQPDSLE GWVPVREGLF AEPERHRLRF LVAWNGAEGK FAVTCHDRTA
60 70 80 90 100
QQRRLREGAR LGPEPEPKPE AAVSPSSWAG LLSAAGLRGA HRQLAALWPP
110 120 130 140 150
LERCFPRLPP ELDVGGGGAW GLGLGLWALL WPTRAGPGEA ALQELCGQLE
160 170 180 190 200
RYLGAAADGC GGATVRDALF PAEGGAADCE SPREFRERAL RARWVEADAR
210 220 230 240 250
LRQVIQGHGK ANTMVALMNV YQEEDEAYQE LVTVATMFFQ YLLQPFRAMR
260 270 280 290 300
EVATLCKLDI LKSLDEDDLG PRRVVALEKE AEEWTRRAEE AVVSIQDITV
310 320 330 340 350
NYFKETVKAL AGMQKEMEQD AKRFGQAAWA TAIPRLEKLQ LMLARETLQL
360 370 380 390 400
MRAKELCLNH KRAEIQGKME DLPEQEKNTN VVDELEIQFY EIQLELYEVK
410 420 430 440 450
FEILKNEEIL LTTQLDSLKR LIKEKQDEVV YYDPCENPEE LKVIDCVVGL
460 470 480 490 500
QDDKNLEVKE LRRQCQQLES KRGRICAKRA SLRSRKDQCK ENHRFRLQQA
510 520 530 540 550
EESIRYSRQH HSIQMKRDKI KEEEQKKKEW INQERQKTLQ RLRSFKDKRL
560 570 580 590 600
AQSVRNTSGS EPVAPNLPSD LSQQMCLPAS HAVSVIHPSS RKTRGVPLSE
610 620 630 640 650
AGNVKSPKCQ NCHGNIPVQV FVPVGDQTHS KSSEELSLPP PPPPPPPPPP
660 670 680 690 700
PPPPPPPPLR ALSSSSQAAT HQNLGFRAPV KDDQPRPLVC ESPAERPRDS
710 720 730 740 750
LESFSCPGSM DEVLASLRHG RAPLRKVEVP AVRPPHASIN EHILAAIRQG
760 770 780 790 800
VKLKKVHPDL GPNPSSKPTS NRRTSDLERS IKAALQRIKR VSADSEEDSD

EQDPGQWDG
Length:809
Mass (Da):90,924
Last modified:July 10, 2007 - v2
Checksum:i29EA39E81A2E309C
GO

Sequence cautioni

The sequence BAB85557 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB85557 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC05201 differs from that shown. Unlikely isoform. Aberrant splice sites.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061721212N → S.Corresponds to variant rs35270670dbSNPEnsembl.1
Natural variantiVAR_033209340Q → K.Corresponds to variant rs1055666dbSNPEnsembl.1
Natural variantiVAR_033210345R → Q.Corresponds to variant rs1055667dbSNPEnsembl.1
Natural variantiVAR_033211686R → H.Corresponds to variant rs3814281dbSNPEnsembl.1
Natural variantiVAR_051489736H → P.Corresponds to variant rs11259953dbSNPEnsembl.1
Natural variantiVAR_051490736H → Q.Corresponds to variant rs11259954dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075851 mRNA. Translation: BAB85557.1. Different initiation.
AC044907 Genomic DNA. No translation available.
AK097947 mRNA. Translation: BAC05201.1. Sequence problems.
CCDSiCCDS45333.1.
RefSeqiNP_001073904.1. NM_001080435.2.
UniGeneiHs.377360.

Genome annotation databases

EnsembliENST00000286760; ENSP00000286760; ENSG00000156232.
GeneIDi123720.
KEGGihsa:123720.
UCSCiuc002bje.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB075851 mRNA. Translation: BAB85557.1. Different initiation.
AC044907 Genomic DNA. No translation available.
AK097947 mRNA. Translation: BAC05201.1. Sequence problems.
CCDSiCCDS45333.1.
RefSeqiNP_001073904.1. NM_001080435.2.
UniGeneiHs.377360.

3D structure databases

ProteinModelPortaliQ8TF30.
SMRiQ8TF30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125830. 1 interactor.
STRINGi9606.ENSP00000286760.

PTM databases

iPTMnetiQ8TF30.
PhosphoSitePlusiQ8TF30.

Polymorphism and mutation databases

BioMutaiWHAMM.
DMDMi152112352.

Proteomic databases

EPDiQ8TF30.
MaxQBiQ8TF30.
PaxDbiQ8TF30.
PeptideAtlasiQ8TF30.
PRIDEiQ8TF30.

Protocols and materials databases

DNASUi123720.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286760; ENSP00000286760; ENSG00000156232.
GeneIDi123720.
KEGGihsa:123720.
UCSCiuc002bje.5. human.

Organism-specific databases

CTDi123720.
DisGeNETi123720.
GeneCardsiWHAMM.
H-InvDBHIX0012510.
HIX0022942.
HIX0038351.
HGNCiHGNC:30493. WHAMM.
HPAiHPA039690.
HPA040231.
MIMi612393. gene.
neXtProtiNX_Q8TF30.
OpenTargetsiENSG00000156232.
PharmGKBiPA164727567.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF4S. Eukaryota.
ENOG4111J4B. LUCA.
GeneTreeiENSGT00510000046704.
HOGENOMiHOG000074181.
HOVERGENiHBG067146.
InParanoidiQ8TF30.
KOiK20479.
OMAiCGQLERY.
OrthoDBiEOG091G0217.
PhylomeDBiQ8TF30.
TreeFamiTF331023.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156232-MONOMER.

Miscellaneous databases

ChiTaRSiWHAMM. human.
GenomeRNAii123720.
PROiQ8TF30.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156232.
CleanExiHS_WHDC1.
GenevisibleiQ8TF30. HS.

Family and domain databases

InterProiIPR031738. JMY/WHAMM.
IPR031808. JMY/WHAMM_N.
IPR003124. WH2_dom.
IPR026688. WHAMM.
[Graphical view]
PANTHERiPTHR23330:SF6. PTHR23330:SF6. 1 hit.
PfamiPF15871. JMY. 1 hit.
PF15920. WHAMM-JMY_N. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWHAMM_HUMAN
AccessioniPrimary (citable) accession number: Q8TF30
Secondary accession number(s): Q8N1J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.