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Protein

Serine/threonine-protein phosphatase 4 regulatory subunit 1

Gene

PPP4R1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3.1 Publication

GO - Molecular functioni

  • protein phosphatase type 4 regulator activity Source: UniProtKB

GO - Biological processi

  • protein dephosphorylation Source: GOC
  • protein phosphorylation Source: UniProtKB
  • signal transduction Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 regulatory subunit 1
Gene namesi
Name:PPP4R1
Synonyms:MEG1, PP4R1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:9320. PPP4R1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33684.

Polymorphism and mutation databases

BioMutaiPPP4R1.
DMDMi44888283.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 950950Serine/threonine-protein phosphatase 4 regulatory subunit 1PRO_0000071527Add
BLAST

Proteomic databases

EPDiQ8TF05.
MaxQBiQ8TF05.
PaxDbiQ8TF05.
PRIDEiQ8TF05.

PTM databases

iPTMnetiQ8TF05.
PhosphoSiteiQ8TF05.

Expressioni

Tissue specificityi

Widely expressed with high expression in cultured mesangial cells. Isoform 1 and isoform 2 are expressed in renal tissues.1 Publication

Gene expression databases

BgeeiQ8TF05.
CleanExiHS_PPP4R1.
ExpressionAtlasiQ8TF05. baseline and differential.
GenevisibleiQ8TF05. HS.

Organism-specific databases

HPAiHPA040905.
HPA041089.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R1. Interacts with HDAC3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP4CP605106EBI-1056262,EBI-1046072

Protein-protein interaction databases

BioGridi115310. 35 interactions.
IntActiQ8TF05. 20 interactions.
MINTiMINT-8215714.
STRINGi9606.ENSP00000383402.

Structurei

3D structure databases

ProteinModelPortaliQ8TF05.
SMRiQ8TF05. Positions 169-323, 497-929.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 2525HEAT 1Add
BLAST
Repeati26 – 6338HEAT 2Add
BLAST
Repeati65 – 8117HEAT 3Add
BLAST
Repeati82 – 11938HEAT 4Add
BLAST
Repeati127 – 16438HEAT 5Add
BLAST
Repeati168 – 20639HEAT 6Add
BLAST
Repeati208 – 24639HEAT 7Add
BLAST
Repeati248 – 28538HEAT 8Add
BLAST
Repeati287 – 32438HEAT 9Add
BLAST
Repeati505 – 54238HEAT 10Add
BLAST
Repeati568 – 60639HEAT 11Add
BLAST
Repeati698 – 73437HEAT 12Add
BLAST
Repeati799 – 83739HEAT 13Add
BLAST
Repeati861 – 89838HEAT 14Add
BLAST

Sequence similaritiesi

Contains 14 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IUDJ. Eukaryota.
ENOG410XQII. LUCA.
GeneTreeiENSGT00730000110899.
HOGENOMiHOG000115650.
HOVERGENiHBG053649.
InParanoidiQ8TF05.
KOiK15424.
OMAiQFAVHLM.
OrthoDBiEOG7RRF6D.
PhylomeDBiQ8TF05.
TreeFamiTF105560.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR031087. PP4R1.
IPR033461. WRNPLPNID.
[Graphical view]
PANTHERiPTHR10648:SF8. PTHR10648:SF8. 2 hits.
PfamiPF02985. HEAT. 2 hits.
PF15017. WRNPLPNID. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TF05-1) [UniParc]FASTAAdd to basket

Also known as: PP4Rmeg

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADLSLLQED LQEDADGFGV DDYSSESDVI IIPSALDFVS QDEMLTPLGR
60 70 80 90 100
LDKYAASENI FNRQMVARSL LDTLREVCDD ERDCIAVLER ISRLADDSEP
110 120 130 140 150
TVRAELMEQV PHIALFCQEN RPSIPYAFSK FLLPIVVRYL ADQNNQVRKT
160 170 180 190 200
SQAALLALLE QELIERFDVE TKVCPVLIEL TAPDSNDDVK TEAVAIMCKM
210 220 230 240 250
APMVGKDITE RLILPRFCEM CCDCRMFHVR KVCAANFGDI CSVVGQQATE
260 270 280 290 300
EMLLPRFFQL CSDNVWGVRK ACAECFMAVS CATCQEIRRT KLSALFINLI
310 320 330 340 350
SDPSRWVRQA AFQSLGPFIS TFANPSSSGQ YFKEESKSSE EMSVENKNRT
360 370 380 390 400
RDQEAPEDVQ VRPEDTPSDL SVSNSSVILE NTMEDHAAEA SGKPLGEISV
410 420 430 440 450
PLDSSLLCTL SSESHQEAAS NENDKKPGNY KSMLRPEVGT TSQDSALLDQ
460 470 480 490 500
ELYNSFHFWR TPLPEIDLDI ELEQNSGGKP SPEGPEEESE GPVPSSPNIT
510 520 530 540 550
MATRKELEEM IENLEPHIDD PDVKAQVEVL SAALRASSLD AHEETISIEK
560 570 580 590 600
RSDLQDELDI NELPNCKINQ EDSVPLISDA VENMDSTLHY IHSDSDLSNN
610 620 630 640 650
SSFSPDEERR TKVQDVVPQA LLDQYLSMTD PSRAQTVDTE IAKHCAYSLP
660 670 680 690 700
GVALTLGRQN WHCLRETYET LASDMQWKVR RTLAFSIHEL AVILGDQLTA
710 720 730 740 750
ADLVPIFNGF LKDLDEVRIG VLKHLHDFLK LLHIDKRREY LYQLQEFLVT
760 770 780 790 800
DNSRNWRFRA ELAEQLILLL ELYSPRDVYD YLRPIALNLC ADKVSSVRWI
810 820 830 840 850
SYKLVSEMVK KLHAATPPTF GVDLINELVE NFGRCPKWSG RQAFVFVCQT
860 870 880 890 900
VIEDDCLPMD QFAVHLMPHL LTLANDRVPN VRVLLAKTLR QTLLEKDYFL
910 920 930 940 950
ASASCHQEAV EQTIMALQMD RDSDVKYFAS IHPASTKISE DAMSTASSTY
Length:950
Mass (Da):107,004
Last modified:June 1, 2002 - v1
Checksum:iE1392E6BAD3B58CB
GO
Isoform 2 (identifier: Q8TF05-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-35: FGVDDYSSESDVIIIPSA → S

Show »
Length:933
Mass (Da):105,195
Checksum:i3AD23605BB2A371A
GO

Sequence cautioni

The sequence AAD43008.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti43 – 431E → K.
Corresponds to variant rs1056191 [ dbSNP | Ensembl ].
VAR_017807
Natural varianti470 – 4701I → V.
Corresponds to variant rs329003 [ dbSNP | Ensembl ].
VAR_017808
Natural varianti593 – 5931S → N.
Corresponds to variant rs2306134 [ dbSNP | Ensembl ].
VAR_051748
Natural varianti595 – 5951S → N.
Corresponds to variant rs2306134 [ dbSNP | Ensembl ].
VAR_017809

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 3518FGVDD…IIPSA → S in isoform 2. 2 PublicationsVSP_009469Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111106 mRNA. Translation: AAD09818.1.
AF200478 mRNA. Translation: AAL78298.1.
AP000902 Genomic DNA. No translation available.
AP001381 Genomic DNA. No translation available.
BC060829 mRNA. Translation: AAH60829.1.
AF100744 mRNA. Translation: AAD43008.1. Different initiation.
U79267 mRNA. Translation: AAB50211.1.
CCDSiCCDS42412.1. [Q8TF05-1]
CCDS42413.1. [Q8TF05-2]
RefSeqiNP_001035847.1. NM_001042388.2. [Q8TF05-1]
NP_005125.1. NM_005134.3. [Q8TF05-2]
UniGeneiHs.743236.

Genome annotation databases

EnsembliENST00000400555; ENSP00000383401; ENSG00000154845. [Q8TF05-2]
ENST00000400556; ENSP00000383402; ENSG00000154845. [Q8TF05-1]
GeneIDi9989.
KEGGihsa:9989.
UCSCiuc002kod.3. human. [Q8TF05-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111106 mRNA. Translation: AAD09818.1.
AF200478 mRNA. Translation: AAL78298.1.
AP000902 Genomic DNA. No translation available.
AP001381 Genomic DNA. No translation available.
BC060829 mRNA. Translation: AAH60829.1.
AF100744 mRNA. Translation: AAD43008.1. Different initiation.
U79267 mRNA. Translation: AAB50211.1.
CCDSiCCDS42412.1. [Q8TF05-1]
CCDS42413.1. [Q8TF05-2]
RefSeqiNP_001035847.1. NM_001042388.2. [Q8TF05-1]
NP_005125.1. NM_005134.3. [Q8TF05-2]
UniGeneiHs.743236.

3D structure databases

ProteinModelPortaliQ8TF05.
SMRiQ8TF05. Positions 169-323, 497-929.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115310. 35 interactions.
IntActiQ8TF05. 20 interactions.
MINTiMINT-8215714.
STRINGi9606.ENSP00000383402.

PTM databases

iPTMnetiQ8TF05.
PhosphoSiteiQ8TF05.

Polymorphism and mutation databases

BioMutaiPPP4R1.
DMDMi44888283.

Proteomic databases

EPDiQ8TF05.
MaxQBiQ8TF05.
PaxDbiQ8TF05.
PRIDEiQ8TF05.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000400555; ENSP00000383401; ENSG00000154845. [Q8TF05-2]
ENST00000400556; ENSP00000383402; ENSG00000154845. [Q8TF05-1]
GeneIDi9989.
KEGGihsa:9989.
UCSCiuc002kod.3. human. [Q8TF05-1]

Organism-specific databases

CTDi9989.
GeneCardsiPPP4R1.
HGNCiHGNC:9320. PPP4R1.
HPAiHPA040905.
HPA041089.
MIMi604908. gene.
neXtProtiNX_Q8TF05.
PharmGKBiPA33684.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUDJ. Eukaryota.
ENOG410XQII. LUCA.
GeneTreeiENSGT00730000110899.
HOGENOMiHOG000115650.
HOVERGENiHBG053649.
InParanoidiQ8TF05.
KOiK15424.
OMAiQFAVHLM.
OrthoDBiEOG7RRF6D.
PhylomeDBiQ8TF05.
TreeFamiTF105560.

Miscellaneous databases

ChiTaRSiPPP4R1. human.
GeneWikiiPPP4R1.
GenomeRNAii9989.
PROiQ8TF05.
SOURCEiSearch...

Gene expression databases

BgeeiQ8TF05.
CleanExiHS_PPP4R1.
ExpressionAtlasiQ8TF05. baseline and differential.
GenevisibleiQ8TF05. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR031087. PP4R1.
IPR033461. WRNPLPNID.
[Graphical view]
PANTHERiPTHR10648:SF8. PTHR10648:SF8. 2 hits.
PfamiPF02985. HEAT. 2 hits.
PF15017. WRNPLPNID. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and identification of a novel subunit of protein serine/threonine phosphatase 4."
    Kloeker S., Wadzinski B.E.
    J. Biol. Chem. 274:5339-5347(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBUNIT.
    Tissue: Neuroepithelium.
  2. "Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells."
    Wada T., Miyata T., Inagi R., Nangaku M., Wagatsuma M., Suzuki D., Wadzinski B.E., Okubo K., Kurokawa K.
    J. Am. Soc. Nephrol. 12:2601-2608(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.
  5. "Human hypothetical 19.5 kDa protein gene."
    Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 571-950 (ISOFORMS 1/2).
    Tissue: Pituitary.
  6. "Large-scale concatenation cDNA sequencing."
    Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.
    Genome Res. 7:353-358(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 778-950 (ISOFORMS 1/2).
    Tissue: Brain.
  7. "Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4."
    Zhang X., Ozawa Y., Lee H., Wen Y.D., Tan T.H., Wadzinski B.E., Seto E.
    Genes Dev. 19:827-839(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HDAC3, FUNCTION OF THE PPP4C-PPP4R1 COMPLEX.
  8. "A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication."
    Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn D.M., Weinstock D.M., Pfeifer G.P., Lieberman J.
    Mol. Cell 31:33-46(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PPP4C-PPP4R1 COMPLEX.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP4R1_HUMAN
AccessioniPrimary (citable) accession number: Q8TF05
Secondary accession number(s): Q99774, Q9UNQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.