Reviewed, UniProtKB/Swiss-Prot Q8TF05 (PP4R1_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 4 regulatory subunit 1

Gene names

Name:	PPP4R1
Synonyms:	MEG1, PP4R1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. Ref.7
Subunit structure	Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R1. Interacts with HDAC3. Ref.7 Ref.1 Ref.2
Tissue specificity	Widely expressed with high expression in cultured mesangial cells. Isoform 1 and isoform 2 are expressed in renal tissues. Ref.2
Sequence similarities	Contains 14 HEAT repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Gene Ontology (GO)
Biological process	signal transduction Ref.1 Non-traceable author statement. Source: UniProtKB
Cellular component	soluble fraction Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 4 regulator activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP4C	P60510	1	EBI-1056262,EBI-1046072

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8TF05-1)

Also known as: PP4Rmeg;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8TF05-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-35: FGVDDYSSESDVIIIPSA → S

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 950	950	Serine/threonine-protein phosphatase 4 regulatory subunit 1		PRO_0000071527
Regions
	Repeat	1 – 25	25	HEAT 1
	Repeat	26 – 63	38	HEAT 2
	Repeat	65 – 81	17	HEAT 3
	Repeat	82 – 119	38	HEAT 4
	Repeat	127 – 164	38	HEAT 5
	Repeat	168 – 206	39	HEAT 6
	Repeat	208 – 246	39	HEAT 7
	Repeat	248 – 285	38	HEAT 8
	Repeat	287 – 324	38	HEAT 9
	Repeat	505 – 542	38	HEAT 10
	Repeat	568 – 606	39	HEAT 11
	Repeat	698 – 734	37	HEAT 12
	Repeat	799 – 837	39	HEAT 13
	Repeat	861 – 898	38	HEAT 14
Natural variations
	Alternative sequence	18 – 35	18	FGVDD…IIPSA → S in isoform 2.		VSP_009469
	Natural variant	43	1	E → K: dbSNP rs1056191.		VAR_017807
	Natural variant	470	1	I → V: dbSNP rs329003.		VAR_017808
	Natural variant	593	1	S → N: dbSNP rs2306134.		VAR_051748
	Natural variant	595	1	S → N: dbSNP rs2306134.		VAR_017809

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PP4Rmeg) [UniParc]. Last modified June 1, 2002. Version 1. Checksum: E1392E6BAD3B58CB	FASTA	950	107,004
	10 20 30 40 50 60 MADLSLLQED LQEDADGFGV DDYSSESDVI IIPSALDFVS QDEMLTPLGR LDKYAASENI 70 80 90 100 110 120 FNRQMVARSL LDTLREVCDD ERDCIAVLER ISRLADDSEP TVRAELMEQV PHIALFCQEN 130 140 150 160 170 180 RPSIPYAFSK FLLPIVVRYL ADQNNQVRKT SQAALLALLE QELIERFDVE TKVCPVLIEL 190 200 210 220 230 240 TAPDSNDDVK TEAVAIMCKM APMVGKDITE RLILPRFCEM CCDCRMFHVR KVCAANFGDI 250 260 270 280 290 300 CSVVGQQATE EMLLPRFFQL CSDNVWGVRK ACAECFMAVS CATCQEIRRT KLSALFINLI 310 320 330 340 350 360 SDPSRWVRQA AFQSLGPFIS TFANPSSSGQ YFKEESKSSE EMSVENKNRT RDQEAPEDVQ 370 380 390 400 410 420 VRPEDTPSDL SVSNSSVILE NTMEDHAAEA SGKPLGEISV PLDSSLLCTL SSESHQEAAS 430 440 450 460 470 480 NENDKKPGNY KSMLRPEVGT TSQDSALLDQ ELYNSFHFWR TPLPEIDLDI ELEQNSGGKP 490 500 510 520 530 540 SPEGPEEESE GPVPSSPNIT MATRKELEEM IENLEPHIDD PDVKAQVEVL SAALRASSLD 550 560 570 580 590 600 AHEETISIEK RSDLQDELDI NELPNCKINQ EDSVPLISDA VENMDSTLHY IHSDSDLSNN 610 620 630 640 650 660 SSFSPDEERR TKVQDVVPQA LLDQYLSMTD PSRAQTVDTE IAKHCAYSLP GVALTLGRQN 670 680 690 700 710 720 WHCLRETYET LASDMQWKVR RTLAFSIHEL AVILGDQLTA ADLVPIFNGF LKDLDEVRIG 730 740 750 760 770 780 VLKHLHDFLK LLHIDKRREY LYQLQEFLVT DNSRNWRFRA ELAEQLILLL ELYSPRDVYD 790 800 810 820 830 840 YLRPIALNLC ADKVSSVRWI SYKLVSEMVK KLHAATPPTF GVDLINELVE NFGRCPKWSG 850 860 870 880 890 900 RQAFVFVCQT VIEDDCLPMD QFAVHLMPHL LTLANDRVPN VRVLLAKTLR QTLLEKDYFL 910 920 930 940 950 ASASCHQEAV EQTIMALQMD RDSDVKYFAS IHPASTKISE DAMSTASSTY
	Isoform 2. Checksum: 3AD23605BB2A371A	FASTA	933	105,195
	10 20 30 40 50 60 MADLSLLQED LQEDADGSLD FVSQDEMLTP LGRLDKYAAS ENIFNRQMVA RSLLDTLREV 70 80 90 100 110 120 CDDERDCIAV LERISRLADD SEPTVRAELM EQVPHIALFC QENRPSIPYA FSKFLLPIVV 130 140 150 160 170 180 RYLADQNNQV RKTSQAALLA LLEQELIERF DVETKVCPVL IELTAPDSND DVKTEAVAIM 190 200 210 220 230 240 CKMAPMVGKD ITERLILPRF CEMCCDCRMF HVRKVCAANF GDICSVVGQQ ATEEMLLPRF 250 260 270 280 290 300 FQLCSDNVWG VRKACAECFM AVSCATCQEI RRTKLSALFI NLISDPSRWV RQAAFQSLGP 310 320 330 340 350 360 FISTFANPSS SGQYFKEESK SSEEMSVENK NRTRDQEAPE DVQVRPEDTP SDLSVSNSSV 370 380 390 400 410 420 ILENTMEDHA AEASGKPLGE ISVPLDSSLL CTLSSESHQE AASNENDKKP GNYKSMLRPE 430 440 450 460 470 480 VGTTSQDSAL LDQELYNSFH FWRTPLPEID LDIELEQNSG GKPSPEGPEE ESEGPVPSSP 490 500 510 520 530 540 NITMATRKEL EEMIENLEPH IDDPDVKAQV EVLSAALRAS SLDAHEETIS IEKRSDLQDE 550 560 570 580 590 600 LDINELPNCK INQEDSVPLI SDAVENMDST LHYIHSDSDL SNNSSFSPDE ERRTKVQDVV 610 620 630 640 650 660 PQALLDQYLS MTDPSRAQTV DTEIAKHCAY SLPGVALTLG RQNWHCLRET YETLASDMQW 670 680 690 700 710 720 KVRRTLAFSI HELAVILGDQ LTAADLVPIF NGFLKDLDEV RIGVLKHLHD FLKLLHIDKR 730 740 750 760 770 780 REYLYQLQEF LVTDNSRNWR FRAELAEQLI LLLELYSPRD VYDYLRPIAL NLCADKVSSV 790 800 810 820 830 840 RWISYKLVSE MVKKLHAATP PTFGVDLINE LVENFGRCPK WSGRQAFVFV CQTVIEDDCL 850 860 870 880 890 900 PMDQFAVHLM PHLLTLANDR VPNVRVLLAK TLRQTLLEKD YFLASASCHQ EAVEQTIMAL 910 920 930 QMDRDSDVKY FASIHPASTK ISEDAMSTAS STY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and identification of a novel subunit of protein serine/threonine phosphatase 4." Kloeker S., Wadzinski B.E. J. Biol. Chem. 274:5339-5347(1999) [PubMed: 10026142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBUNIT. Tissue: Neuroepithelium.
[2]	"Cloning and characterization of a novel subunit of protein serine/threonine phosphatase 4 from mesangial cells." Wada T., Miyata T., Inagi R., Nangaku M., Wagatsuma M., Suzuki D., Wadzinski B.E., Okubo K., Kurokawa K. J. Am. Soc. Nephrol. 12:2601-2608(2001) [PubMed: 11729228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, TISSUE SPECIFICITY.
[3]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[5]	"Human hypothetical 19.5 kDa protein gene." Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 571-950 (ISOFORMS 1/2). Tissue: Pituitary.
[6]	"Large-scale concatenation cDNA sequencing." Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A. Genome Res. 7:353-358(1997) [PubMed: 9110174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 778-950 (ISOFORMS 1/2). Tissue: Brain.
[7]	"Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4." Zhang X., Ozawa Y., Lee H., Wen Y.D., Tan T.H., Wadzinski B.E., Seto E. Genes Dev. 19:827-839(2005) [PubMed: 15805470] [Abstract] Cited for: INTERACTION WITH HDAC3, FUNCTION OF THE PPP4C-PPP4R1 COMPLEX.
[8]	"A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication." Chowdhury D., Xu X., Zhong X., Ahmed F., Zhong J., Liao J., Dykxhoorn D.M., Weinstock D.M., Pfeifer G.P., Lieberman J. Mol. Cell 31:33-46(2008) [PubMed: 18614045] [Abstract] Cited for: IDENTIFICATION IN THE PPP4C-PPP4R1 COMPLEX.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF111106 mRNA. Translation: AAD09818.1. AF200478 mRNA. Translation: AAL78298.1. AP000902 Genomic DNA. No translation available. AP001381 Genomic DNA. No translation available. BC060829 mRNA. Translation: AAH60829.1. AF100744 mRNA. Translation: AAD43008.1. Different initiation. U79267 mRNA. Translation: AAB50211.1.
IPI	IPI00411730. IPI00478255.
RefSeq	NP_001035847.1. NP_005125.1.
UniGene	Hs.464595
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8TF05. 3 interactions.
Proteomic databases
PRIDE	Q8TF05.
Genome annotation databases
Ensembl	ENSG00000154845. Homo sapiens. [Contig view]
GeneID	9989.
KEGG	hsa:9989.
NMPDR	fig|9606.3.peg.14775.
Organism-specific databases
GeneCards	GC18M009536.
H-InvDB	HIX0014326.
HGNC	HGNC:9320. PPP4R1.
MIM	604908. gene.
PharmGKB	PA33684.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8TF05.
HOVERGEN	Q8TF05.
Gene expression databases
ArrayExpress	Q8TF05.
Bgee	Q8TF05.
CleanEx	HS_PPP4R1.
GermOnline	ENSG00000154845. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR000357. HEAT. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF02985. HEAT. 5 hits. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37723.
SOURCE	Search...

Entry information

Entry name

PP4R1_HUMAN

Accession

Primary (citable) accession number: Q8TF05
Secondary accession number(s): Q99774, Q9UNQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2002
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)