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Protein

Importin-4

Gene

IPO4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-4
Short name:
Imp4
Alternative name(s):
Importin-4b
Short name:
Imp4b
Ran-binding protein 4
Short name:
RanBP4
Gene namesi
Name:IPO4
Synonyms:IMP4B, RANBP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:19426. IPO4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • membrane Source: UniProtKB
  • nuclear chromatin Source: UniProtKB
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000196497.
PharmGKBiPA134968932.

Polymorphism and mutation databases

BioMutaiIPO4.
DMDMi126302558.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207481 – 1081Importin-4Add BLAST1081

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8TEX9.
MaxQBiQ8TEX9.
PaxDbiQ8TEX9.
PeptideAtlasiQ8TEX9.
PRIDEiQ8TEX9.

PTM databases

iPTMnetiQ8TEX9.
PhosphoSitePlusiQ8TEX9.

Expressioni

Gene expression databases

BgeeiENSG00000196497.
CleanExiHS_IPO4.
ExpressionAtlasiQ8TEX9. baseline and differential.
GenevisibleiQ8TEX9. HS.

Organism-specific databases

HPAiHPA039043.
HPA039044.

Interactioni

Subunit structurei

Binds with high affinity to RPS3A. The binding is coupled to RanGTP cycles. Binds to human cytomegalovirus UL84. Found in a cytosolic complex with ASF1A, ASF1B, CDAN1 and histones H3.1 and H4.1 Publication

Protein-protein interaction databases

BioGridi122828. 70 interactors.
DIPiDIP-32944N.
IntActiQ8TEX9. 31 interactors.
MINTiMINT-3045897.
STRINGi9606.ENSP00000346453.

Structurei

3D structure databases

ProteinModelPortaliQ8TEX9.
SMRiQ8TEX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 90Importin N-terminalPROSITE-ProRule annotationAdd BLAST67
Repeati348 – 385HEAT 1Add BLAST38
Repeati390 – 427HEAT 2Add BLAST38
Repeati431 – 471HEAT 3Add BLAST41
Repeati475 – 513HEAT 4Add BLAST39
Repeati895 – 932HEAT 5Add BLAST38
Repeati936 – 974HEAT 6Add BLAST39

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 6 HEAT repeats.PROSITE-ProRule annotation
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQKY. Eukaryota.
ENOG410XP2Z. LUCA.
GeneTreeiENSGT00550000075074.
HOVERGENiHBG049052.
InParanoidiQ8TEX9.
KOiK20221.
OMAiRPAQEYF.
OrthoDBiEOG091G012W.
PhylomeDBiQ8TEX9.
TreeFamiTF323157.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8TEX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESAGLEQLL RELLLPDTER IRRATEQLQI VLRAPAALPA LCDLLASAAD
60 70 80 90 100
PQIRQFAAVL TRRRLNTRWR RLAAEQRESL KSLILTALQR ETEHCVSLSL
110 120 130 140 150
AQLSATIFRK EGLEAWPQLL QLLQHSTHSP HSPEREMGLL LLSVVVTSRP
160 170 180 190 200
EAFQPHHREL LRLLNETLGE VGSPGLLFYS LRTLTTMAPY LSTEDVPLAR
210 220 230 240 250
MLVPKLIMAM QTLIPIDEAK ACEALEALDE LLESEVPVIT PYLSEVLTFC
260 270 280 290 300
LEVARNVALG NAIRIRILCC LTFLVKVKSK ALLKNRLLPP LLHTLFPIVA
310 320 330 340 350
AEPPPGQLDP EDQDSEEEEL EIELMGETPK HFAVQVVDML ALHLPPEKLC
360 370 380 390 400
PQLMPMLEEA LRSESPYQRK AGLLVLAVLS DGAGDHIRQR LLPPLLQIVC
410 420 430 440 450
KGLEDPSQVV RNAALFALGQ FSENLQPHIS SYSREVMPLL LAYLKSVPLG
460 470 480 490 500
HTHHLAKACY ALENFVENLG PKVQPYLPEL MECMLQLLRN PSSPRAKELA
510 520 530 540 550
VSALGAIATA AQASLLPYFP AIMEHLREFL LTGREDLQPV QIQSLETLGV
560 570 580 590 600
LARAVGEPMR PLAEECCQLG LGLCDQVDDP DLRRCTYSLF AALSGLMGEG
610 620 630 640 650
LAPHLEQITT LMLLSLRSTE GIVPQYDGSS SFLLFDDESD GEEEEELMDE
660 670 680 690 700
DVEEEDDSEI SGYSVENAFF DEKEDTCAAV GEISVNTSVA FLPYMESVFE
710 720 730 740 750
EVFKLLECPH LNVRKAAHEA LGQFCCALHK ACQSCPSEPN TAALQAALAR
760 770 780 790 800
VVPSYMQAVN RERERQVVMA VLEALTGVLR SCGTLTLKPP GRLAELCGVL
810 820 830 840 850
KAVLQRKTAC QDTDEEEEEE DDDQAEYDAM LLEHAGEAIP ALAAAAGGDS
860 870 880 890 900
FAPFFAGFLP LLVCKTKQGC TVAEKSFAVG TLAETIQGLG AASAQFVSRL
910 920 930 940 950
LPVLLSTAQE ADPEVRSNAI FGMGVLAEHG GHPAQEHFPK LLGLLFPLLA
960 970 980 990 1000
RERHDRVRDN ICGALARLLM ASPTRKPEPQ VLAALLHALP LKEDLEEWVT
1010 1020 1030 1040 1050
IGRLFSFLYQ SSPDQVIDVA PELLRICSLI LADNKIPPDT KAALLLLLTF
1060 1070 1080
LAKQHTDSFQ AALGSLPVDK AQELQAVLGL S
Length:1,081
Mass (Da):118,715
Last modified:February 20, 2007 - v2
Checksum:i36D11AF0D6DA8B1E
GO
Isoform 2 (identifier: Q8TEX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1038-1038: P → PGE

Note: No experimental confirmation available.
Show »
Length:1,083
Mass (Da):118,901
Checksum:iBBB539F050812D96
GO

Sequence cautioni

The sequence BAB15616 differs from that shown. Reason: Frameshift at position 234.Curated
The sequence BAB55421 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC11174 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAD62595 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti865K → R in BAB15616 (PubMed:14702039).Curated1
Sequence conflicti943G → E in BAB55421 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030758513A → V.2 PublicationsCorresponds to variant rs7146310dbSNPEnsembl.1
Natural variantiVAR_030759580P → A.2 PublicationsCorresponds to variant rs11550452dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0093391038P → PGE in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411122 mRNA. Translation: AAL78660.1.
BX248267 mRNA. Translation: CAD62595.1. Different initiation.
AL136295 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66078.1.
BC003690 mRNA. Translation: AAH03690.2.
BC110804 mRNA. Translation: AAI10805.1.
BC136759 mRNA. Translation: AAI36760.1.
AK026991 mRNA. Translation: BAB15616.1. Frameshift.
AK027871 mRNA. Translation: BAB55421.1. Different initiation.
AK074743 mRNA. Translation: BAC11174.1. Different initiation.
CCDSiCCDS9616.1. [Q8TEX9-1]
RefSeqiNP_078934.3. NM_024658.3. [Q8TEX9-1]
UniGeneiHs.411865.

Genome annotation databases

EnsembliENST00000354464; ENSP00000346453; ENSG00000196497. [Q8TEX9-1]
GeneIDi79711.
KEGGihsa:79711.
UCSCiuc001wmv.2. human. [Q8TEX9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411122 mRNA. Translation: AAL78660.1.
BX248267 mRNA. Translation: CAD62595.1. Different initiation.
AL136295 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66078.1.
BC003690 mRNA. Translation: AAH03690.2.
BC110804 mRNA. Translation: AAI10805.1.
BC136759 mRNA. Translation: AAI36760.1.
AK026991 mRNA. Translation: BAB15616.1. Frameshift.
AK027871 mRNA. Translation: BAB55421.1. Different initiation.
AK074743 mRNA. Translation: BAC11174.1. Different initiation.
CCDSiCCDS9616.1. [Q8TEX9-1]
RefSeqiNP_078934.3. NM_024658.3. [Q8TEX9-1]
UniGeneiHs.411865.

3D structure databases

ProteinModelPortaliQ8TEX9.
SMRiQ8TEX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122828. 70 interactors.
DIPiDIP-32944N.
IntActiQ8TEX9. 31 interactors.
MINTiMINT-3045897.
STRINGi9606.ENSP00000346453.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiQ8TEX9.
PhosphoSitePlusiQ8TEX9.

Polymorphism and mutation databases

BioMutaiIPO4.
DMDMi126302558.

Proteomic databases

EPDiQ8TEX9.
MaxQBiQ8TEX9.
PaxDbiQ8TEX9.
PeptideAtlasiQ8TEX9.
PRIDEiQ8TEX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354464; ENSP00000346453; ENSG00000196497. [Q8TEX9-1]
GeneIDi79711.
KEGGihsa:79711.
UCSCiuc001wmv.2. human. [Q8TEX9-1]

Organism-specific databases

CTDi79711.
GeneCardsiIPO4.
HGNCiHGNC:19426. IPO4.
HPAiHPA039043.
HPA039044.
neXtProtiNX_Q8TEX9.
OpenTargetsiENSG00000196497.
PharmGKBiPA134968932.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IQKY. Eukaryota.
ENOG410XP2Z. LUCA.
GeneTreeiENSGT00550000075074.
HOVERGENiHBG049052.
InParanoidiQ8TEX9.
KOiK20221.
OMAiRPAQEYF.
OrthoDBiEOG091G012W.
PhylomeDBiQ8TEX9.
TreeFamiTF323157.

Miscellaneous databases

ChiTaRSiIPO4. human.
GeneWikiiIPO4.
GenomeRNAii79711.
PROiQ8TEX9.

Gene expression databases

BgeeiENSG00000196497.
CleanExiHS_IPO4.
ExpressionAtlasiQ8TEX9. baseline and differential.
GenevisibleiQ8TEX9. HS.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
PF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50077. HEAT_REPEAT. 2 hits.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPO4_HUMAN
AccessioniPrimary (citable) accession number: Q8TEX9
Secondary accession number(s): B2RN95
, Q2NL96, Q86TZ9, Q8NCG8, Q96SJ3, Q9BTI4, Q9H5L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.